PE Mouse anti-Human IL-17F

BD Pharmingen™ PE Mouse anti-Human IL-17F

Name: PE Mouse anti-Human IL-17F
Brand: BD Pharmingen™
SKU: 561198

クローン O33-782

(RUO)

Flow cytometric analysis of IL-17F expression by resting and activated human peripheral blood CD4+ T cells and Th17 polarized cells. Human peripheral blood mononuclear cells were either unstimulated (Left Panels) or stimulated with Phorbol 12-Myristate 13-Acetate (PMA; Sigma P-8139) plus Ionomycin (Sigma; I-0634) in the presence of BD GolgiStop™ Protein Transport Inhibitor (Cat. No. 554724) for 5 hours (Middle Panels) or were cultured in Th17 polarization conditions and restimulated with PMA and Ionomycin in the presence of BD GolgiStop™ for 5 hours (Right Panels). Cells were then fixed and permeabilized using BD Cytofix/Cytoperm™ reagents (Cat. No. 554714) followed by staining with PE Mouse anti-Human IL-17F (Cat. No. 561197/561198), PerCP-Cy5.5 Mouse anti-Human CD4 (Cat. No. 341654), and FITC Mouse Anti-Human IFN-γ (Cat. No. 554700) or Alexa Fluor® 647 Mouse anti-Human IL-17A (Cat. No. 560490). Two-color flow cytometric dot plots showing the correlated expression patterns of IL-17F versus IL-17A or IFN-γ were derived from CD4+ gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System. Other compatible fixation and permeabilization treatments are listed in the \"Recommended Assay Procedure.\"

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製品詳細

Brand: BD Pharmingen™

Alternative Name: Interleukin-17F; ML-1; cytokine ML-1

Reactivity: Human (QC Testing)

Isotype: Mouse IgG1, κ

Immunogen: Human IL-17F

Application: Intracellular staining (flow cytometry) (Routinely Tested)

Vol. Per Test: 5 µl

RRID: AB_10611873

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

推奨アッセイ手順

This antibody conjugate is suitable for intracellular staining of human peripheral blood mononuclear cells using BD Cytofix/Cytoperm™ reagents, BD Pharmingen™ Human FoxP3 Buffer Set or BD™ Phosflow fixation and permeabilization buffers (Fix buffer I with Perm/Wash Buffer I, Perm Buffer II, or Perm Buffer III).

Product Notices

This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
An isotype control should be used at the same concentration as the antibody of interest.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
Cy is a trademark of GE Healthcare.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

関連製品

Fixation/Permeabilization Kit RUO

サイズ 250 Tests カタログ番号 554714

Fix Buffer I RUO

サイズ 250 mL カタログ番号 557870

Perm/Wash Buffer I RUO

サイズ 125 mL カタログ番号 557885

Perm Buffer II RUO

サイズ 125 mL カタログ番号 558052

Perm Buffer III RUO

サイズ 125 mL カタログ番号 558050

Protein Transport Inhibitor (Containing Monensin) RUO

サイズ 0.7 mL カタログ番号 554724

詳細を表示

561198 Rev. 1

抗体の詳細

O33-782

The O33-782 monoclonal antibody specifically binds to Interleukin-17F (IL-17F). IL-17F is a member of the IL-17 family of cytokines. IL-17F is encoded by the IL17F gene located in chromosome 6 (location: 6p12). IL-17F is a proinflammatory cytokine that is produced by activated T cells including differentiated CD4+ T helper 17 (Th17) cells. Activated Th17 cells can express disulfide-linked IL-17F and IL-17A homodimers as well as IL-17A/IL-17F heterodimers. These IL-17 dimers act by binding to and signaling through IL-17 receptor complexes (IL-17R). IL-17R are comprised of transmembrane IL-17RA and IL-17-RC protein subunits that are expressed by a variety of target cells including epithelial and endothelial cells, keratinocytes, fibroblasts, and granulocytes. IL-17F can induce target cells to produce proinflammatory cytokines such as IL-1β, IL-6, G-CSF, GM-CSF, and TNF and chemokines including CXCL1/Gro-α, CXCL2/Gro-β, and CXCL8/IL-8 that attract and activate leukocytes, eg, neutrophils. Th17 and other IL-17F-producing cells play protective roles in the clearance of extracellular pathogens, including bacteria and fungi. IL-17F can also play adverse roles in inflammation associated with asthma and autoimmune diseases.

561198 Rev. 1

フォーマットの詳細

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

フォーマット: PE

励起源: Yellow-Green 488 nm, 532 nm, 561 nm

最大励起波長: 496 nm, 566 nm

最大蛍光波長: 576 nm

561198 Rev.1

引用＆参考文献

Development References (6)

Fouser LA, Wright JF, Dunussi-Joannopoulos K, Collins M. Th17 cytokines and their emerging roles in inflammation and autoimmunity. Immunol Rev. 2008; 226:87-102. (Biology). View Reference
Shen F, Gaffen SL. Structure-function relationships in the IL-17 receptor: implications for signal transduction and therapy. Cytokine. 2008; 41(2):92-104. (Biology). View Reference
Starnes T, Robertson MJ, Sledge G, et al.. Cutting edge: IL-17F, a novel cytokine selectively expressed in activated T cells and monocytes, regulates angiogenesis and endothelial cell cytokine production. J Immunol. 2001; 167(8):4137-4140. (Biology). View Reference
Wang YH, Liu YJ. The IL-17 cytokine family and their role in allergic inflammation. Curr Opin Immunol. 2008; 20(6):697-702. (Biology). View Reference
Wright JF, Bennett F, Li B, et al. The human IL-17F/IL-17A heterodimeric cytokine signals through the IL-17RA/IL-17RC receptor complex. J Immunol. 2008; 181(4):2799-2805. (Biology). View Reference
Wright JF, Guo Y, Quazi A, et al. Identification of an interleukin 17F/17A heterodimer in activated human CD4+ T cells. J Biol Chem. 2007; 282(18):13447-13455. (Biology). View Reference

すべて表示する (6)

561198 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.