Spectral Flow Cytometry

Do you want to transition from conventional to spectral flow cytometry? Or maybe you’re already doing spectral flow cytometry and want to improve the quality of your data? Gain a deeper understanding of spectral flow cytometry by going back to the fundamentals. Join Peter Mage for an engineer’s perspective on the science of spectral flow cytometry as he explains how the concepts at the heart of spectral technology can inform your experiment design.

In this webinar, he’ll discuss:

• The relationship between conventional and spectral flow cytometry technology
• The difference between conventional compensation and spectral unmixing
• How to build an intuitive mental model for spectral flow cytometry that can help you understand your experiments and data in a new light

High-dimensional immunoprofiling is essential for studying host response to immunotherapy, infection and disease in murine model systems. However, the difficulty of multiparameter panel design combined with a lack of existing murine tools has prevented the comprehensive study of all major leukocyte phenotypes in a single assay.

To fill this niche, we present a 40-color flow cytometry panel for deep immunophenotyping in primary and secondary lymphoid tissues. Importantly, the panel solely relies on extracellular staining to preserve cells for downstream experiments and can identify functional lymphocyte and myeloid subsets. In this webinar, we will discuss how the panel was engineered, as well as special considerations in reagent optimization, spectral unmixing, autofluorescence extraction and multi-dimensional analysis.

Real-time image feature extraction enables novel avenues of research and sort possibilities as we can classify and characterize cell populations based on spatial distribution of fluorescence. The possibilities broaden further when these imaging capabilities are coupled with next-generation spectral immunophenotyping. Although spectral cytometry can seem intimidating or complex, our advanced spectral cytometry system, BD FACSDiscover^™ S8 Cell Sorter with BD CellView^™ Image Technology and BD SpectralFX^™ Technology, combines full-spectrum optics and optimized hardware design with a system-aware algorithm, next-generation quality control (QC) and guided software workflow so users can get started quickly.

In this webinar, Aaron Middlebrook, Sr. Staff Scientist, R&D, BD, and Peter Mage, Associate Principal Engineer, Advanced Technology Group, BD, will demonstrate the power of real-time imaging, spectral flow cytometry (RTI-SFC) and share best practices in experimental setup and examples of applications using this novel technology.