Custom BD^® AbSeq Reagents

Please contact BDB_Custom_Orders@BD.com for all inquiries and requests for BD^® AbSeq Custom Conjugations. Below, is an order form to be completed with the custom conjugation request.

BD^® AbSeq Custom Reagent Request Form

BD Biosciences offers the small-scale custom conjugation of an AbSeq-qualified oligonucleotide barcode to either antibodies provided by customers or BD antibody clones not currently commercialized in the AbSeq portfolio.

There is no minimum or limit to the number of conjugates requested per order.

Yes, requests will be evaluated on a case-by-case basis assessing technical compatibility. BD^® AbSeq Custom Conjugation materials are offered at an optimal concentration, with 25 x 2 µL tests per vial. The optimal concentration is derived from titration analysis via flow cytometry using a fluorochrome labeled format of the antibody.

The cost of a BD^® AbSeq Custom Conjugate of an existing BD clone is US $585 per 25 test size.

We have not fully tested and validated the performance of intracellular markers in the AbSeq workflow, therefore do not currently support AbSeq antibodies against intracellular targets. However, if a customer still wishes to procure an intracellular antibody in AbSeq format, we are happy to support this request via our Customs Conjugation team on a case-by-case basis. Fulfillment of such requests requires acknowledgement of above risks from the requesting customer. We currently do not offer any technical support for intracellular AbSeq workflow. 

Lead times are typically 4 weeks. We will communicate approximate lead times when the quote is made as well as provide follow up communication regarding expected dates of delivery.

BD will provide the entire yield of the custom conjugation for customer provided material at a standard concentration of 0.5 mg/mL. If the material is from a BD clone it will be provided at a 25 test size. The suggested working concentration is 2.0 µL/test reaction. An alternative formulation can be provided upon request from the customer.

BD^® AbSeq Oligonucleotide barcodes are quality controlled and validated to ensure optimal performance and compatibility. At this time, we cannot accommodate specific AbSeq barcode pairing. For customer-provided materials, assigned oligonucleotide barcodes are unique to each customer incorporated into BD bioinformatics tools for seamless analysis. 

BD^® AbSeq Oligonucleotide barcodes are quality controlled and validated to ensure optimal performance and compatibility. All AbSeq barcodes are incorporated into BD bioinformatics tools for seamless analysis. Unfortunately, we do not currently offer custom conjugation service for alternative oligonucleotide sequences.

The sequence barcode belonging to each antibody will be noted in the product information sheet. In addition to being provided with a reference file used in downstream analysis.

Every batch of oligonucleotides used in antibody labeling undergo next-generation sequencing analysis to ensure the sequence, quality and purity. BD^® AbSeq Custom Antibodies are subjected to the same in-process testing for conjugation efficiency as the made-to-stock BD^® AbSeq Reagents. Our proprietary production process has undergone stringent validation procedures and has been used in our entire portfolio of AbSeq products. This gives us confidence in our ability to successfully conjugate a wide variety of antibodies to oligonucleotides with consistent performance characteristics.

If the customer wishes to perform their own QC experiment, we can provide the protocol document titled “Flow Cytometry Analysis of BD^® AbSeq Ab-Oligo and Sample Tag Expression Protocol” for testing of Ab-oligos on cells.

Flow Cytometry Analysis of BD^® AbSeq Ab-Oligo and Sample Tag Expression Protocol

Use of BD^® AbSeq Custom Antibodies on other single-cell platforms is not currently supported and BD makes no guarantees for the performance on other systems.

There is a reference file available to customers for BD^® AbSeq Custom Oligos, which can be found at:
https://s3.amazonaws.com/bd-rhapsody-public/AbSeq-references/BDAbSeq_allReference_latest.fasta

We recommend that customers extract the header names and barcodes from this file that they actually used in the experiment and create a new FASTA file for their particular experiment. Custom customers may also want to add an identifying name to the header line (>) to include the specificity they have had conjugated to the oligo so the identifier will generate in the output files of the pipeline. Please be advised that there should not be a blank space separating the identifier from the oligo ID.