R718 Rat Anti-Mouse CD140b (PDGFRβ)

BD Horizon™ R718 Rat Anti-Mouse CD140b (PDGFRβ)

Name: R718 Rat Anti-Mouse CD140b (PDGFRβ)
Brand: R718 Rat Anti-Mouse CD140b (PDGFRβ)
SKU: 570046

クローン APB5 (RUO)

Multicolor flow cytometric analysis of the correlated expression of CD140a and CD140b (PDGFRβ) on Mouse NIH/3T3 cells. Cells from the Mouse NIH/3T3 (Mouse embryonic fibroblast, ATCC® CRL-1658™) cell line were stained with BD Horizon™ R718 Rat IgG2a, κ Isotype Control (Cat. No. 566941) and PE Rat IgG2a, κ Isotype Control (Cat. No. 553930) [Left Plot] or with BD Horizon™ R718 Rat Anti-Mouse CD140b (PDGFRβ) antibody (Cat. No. 570046/570127) and PE Rat Anti-Mouse CD140a antibody (Cat. No. 562776) [Right Plot] at 0.25 µg/test. DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD140b (PDGFRβ) versus CD140a (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.

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Flow cytometric analysis of CD140b (PDGFRβ) expression on Mouse splenic leukocytes and Mouse NIH/3T3 cells. C57BL/6 Mouse splenocytes (Left Plot) were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553141/553142]. The splenic leukocytes and cells from the Mouse NIH/3T3 (Mouse embryonic fibroblast, ATCC® CRL-1658™) cell line (Right Plot) were then stained with either BD Horizon™ R718 Rat IgG2a, κ Isotype Control (Cat. No. 566941; dashed line histograms) or BD Horizon™ R718 Rat Anti-Mouse CD140b (PDGFRβ) antibody (Cat. No. 570046/570127; solid line histograms) at 0.25 µg/test. DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histograms showing CD140b (PDGFRβ) expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.

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カタログ番号:745296

希望小売価格:¥78,650

単位:EA (1 Each)

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製品詳細

Brand: BD Horizon™

Alternative Name: CD140b; PDGF-R-beta; PDGFR-beta; Pdgfrb; platelet-derived growth factor receptor beta

Reactivity: Mouse (QC Testing)

Isotype: Rat WI, also known as Wistar (outbred) IgG2a, κ

Immunogen: Recombinant Mouse PDGFRβ Extracellular Domain Protein

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

Entrez Gene ID: 18596

RRID: AB_3685482

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

推奨アッセイ手順

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
This product is provided under an Agreement between BIOTIUM and BD Biosciences. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
Alexa Fluor™ is a trademark of Life Technologies Corporation.
For U.S. patents that may apply, see bd.com/patents.

データシート

Technical Data Sheet (TDS)

関連製品

Stain Buffer (FBS) RUO

サイズ 500 mL カタログ番号 554656

Stain Buffer (BSA) RUO

サイズ 500 mL カタログ番号 554657

Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO

サイズ 0.5 mg カタログ番号 553142

R718 Rat IgG2a, κ Isotype Control RUO

サイズ 50 µg カタログ番号 566941

PE Rat IgG2a, κ Isotype Control RUO

サイズ 0.1 mg カタログ番号 553930

PE Rat Anti-Mouse CD140A RUO

サイズ 50 µg カタログ番号 562776

詳細を表示

570046 Rev. 1

抗体の詳細

APB5

The APB5 monoclonal antibody specifically recognizes CD140b, the Platelet-Derived Growth Factor Receptor beta chain (PDGFRβ). CD140b is a single-pass type I transmembrane glycoprotein that contains an intracellular tyrosine kinase domain. This receptor is encoded by Pdgfrb and is widely expressed on cells within embryonic tissues and cells of mesenchymal origin in the adult mouse including fibroblasts and vascular smooth muscle cells. CD140b forms homodimers or heterodimeric receptor complexes with CD140a (PDGFRα) upon binding various dimeric Platelet-Derived Growth Factors (PDGF) isoforms that lead to receptor activation. Upon activation, CD140b is autophosphorylated at multiple tyrosine sites and, in turn, initiates several signaling cascades by binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules, such as GRB2, Src, GAP, PI3 kinase, PLCγ, and NCK. These signaling pathways regulate cellular growth and proliferation, survival, differentiation, and migration involved in the development of embryonic tissues, blood vessel formation, wound healing, and in a range of diseases including fibrotic conditions, atherosclerosis, and malignancies.

570046 Rev. 1

フォーマットの詳細

R718

The BD Horizon™ Red 718 (R718) Dye is part of the BD red family of dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 695-nm and an emission maximum (Em Max) at 718-nm. Driven by BD innovation, R718 is designed to be excited by the red laser (627–640-nm) and detected using an optical filter centered near 720-nm (e.g., a 720/40-nm bandpass filter). R718 is a brighter alternative to Alexa Fluor™ 700. R718 is also a bright small molecule alternative to APC-R700 with lower spread into the APC detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

フォーマット: R718

励起源: Red 627-640 nm

最大励起波長: 695 nm

最大蛍光波長: 718 nm

570046 Rev.1

引用＆参考文献

Development References (7)

Andrae J, Gallini R, Betsholtz C. Role of platelet-derived growth factors in physiology and medicine.. Genes Dev. 2008; 22(10):1276-312. (Biology). View Reference
Cossarizza A, Chang HD, Radbruch A, et al. Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition).. Eur J Immunol. 2019; 49(10):1457-1973. (Clone-specific). View Reference
Cremasco V, Woodruff MC, Onder L, et al. B cell homeostasis and follicle confines are governed by fibroblastic reticular cells.. Nat Immunol. 2014; 15(10):973-81. (Clone-specific: Flow cytometry). View Reference
Ostman A, Heldin CH. Involvement of platelet-derived growth factor in disease: development of specific antagonists.. Adv Cancer Res. 2001; 80:1-38. (Biology). View Reference
Patenaude J, Perreault C. Thymic Mesenchymal Cells Have a Distinct Transcriptomic Profile.. J Immunol. 2016; 196(11):4760-70. (Clone-specific: Flow cytometry). View Reference
Sano H, Sudo T, Yokode M, et al. Functional blockade of platelet-derived growth factor receptor-beta but not of receptor-alpha prevents vascular smooth muscle cell accumulation in fibrous cap lesions in apolipoprotein E-deficient mice.. Circulation. 2001; 103(24):2955-60. (Immunogen: Functional assay, Immunohistochemistry, Western blot). View Reference
Sano H, Yokode M, Takakura N, et al. Study on PDGF receptor beta pathway in glomerular formation in neonate mice.. Ann N Y Acad Sci. 2001; 947:303-5. (Clone-specific: Immunohistochemistry, Western blot). View Reference

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570046 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.