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Flow Cytometric Analysis of CD369 (Clec7a) Expression Top plots - CD369 (Clec7a) expression on Mouse peripheral blood leucocytes (PBL): BALB/c (Panel 1) and C57BL/6 (Panel 2) mouse peripheral blood leucocytes (PBL) were stained with APC Rat Anti-Mouse Ly-6G antibody (Cat. No. 560599) and with either PE mouse IgG1, κ Isotype Control (Cat. No. 550083; Plots A&C) or PE Mouse Anti-Mouse CD369 (Clec7a) antibody (Cat. No. 568714; Plots B&D) at 1.0 ug/test. The bivariate pseudocolor density plots showing the correlated expression of CD369 (Clec7a) [or Ig isotype control staining] versus Ly-6G were derived from gated events with the forward and side-light scatter characteristics of viable leucocyte populations. Bottom plots - CD369 (Clec 7a) expression on Mouse peritoneal exudate cells (PEC): BALB/c (Panel 1) and C57BL/6 (Panel 2) mouse thioglycolate-elicited PEC cells. were harvested and pre-incubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553141/553142]. The cells were then stained with Alexa Fluor™ Rat Anti-Mouse F4/80 antibody (Cat. No. 565854) and with either PE mouse IgG1, κ Isotype Control (Cat. No. 550083; Plots E&G) or PE Mouse Anti-Mouse CD369 (Clec7a) antibody (Cat. No. 568714; Plots F&H) at 1.0 ug/test. The bivariate pseudocolor density plots showing the correlated expression of CD369 (Clec7a) [or Ig isotype control staining] versus F4/80 were derived from gated events with the forward and side-light scatter characteristics of viable PEC cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
BD Pharmingen™ PE Mouse Anti-Mouse CD369 (Clec7a)
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Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation and Storage
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BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
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- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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- An isotype control should be used at the same concentration as the antibody of interest.
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関連製品
The 2F3 monoclonal antibody specifically recognizes CD369 which is also known as Dectin-1 (Dendritic cell-associated C-type lectin 1) or BGR (Beta-glucan receptor). CD369 is a ~ 43 kDa type II transmembrane C-type lectin that is encoded by Clec7a (C-type lectin domain family 7, member a). The extracellular portion of CD369 (Clec7a) contains a C-terminal stalk with a carbohydrate recognition domain (CRD) that is followed by a transmembrane segment, and an ITAM-containing cytoplasmic tail. This protein is predominantly expressed on monocytes/macrophages, neutrophils and some dendritic cell populations and at a lower level on a sub-population of T cells. CD369 (Clec7a) binds to beta-glucan polymers and functions as a pattern recognition receptor (PRR) in innate immune response to fungal and bacterial pathogens. CD369 mediated signaling may play a role in leucocyte responses, including phagocytosis or enhanced cytokine production. In mice, two functionally different full-length and stalkless isoforms have been characterized. Both isoforms vary in their ability to recognize zymosan and mediate cellular responses upon zymosan recognition.
Development References (4)
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Brown GD, Taylor PR, Reid DM, et al. Dectin-1 is a major beta-glucan receptor on macrophages.. J Exp Med. 2002; 196(3):407-12. (Biology). View Reference
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Herre J, Gordon S, Brown GD. Dectin-1 and its role in the recognition of beta-glucans by macrophages.. Mol Immunol. 2004; 40(12):869-76. (Biology). View Reference
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Taylor PR, Brown GD, Reid DM, et al. The beta-glucan receptor, dectin-1, is predominantly expressed on the surface of cells of the monocyte/macrophage and neutrophil lineages.. J Immunol. 2002; 169(7):3876-82. (Biology). View Reference
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Underhill DM, Rossnagle E, Lowell CA, Simmons RM. Dectin-1 activates Syk tyrosine kinase in a dynamic subset of macrophages for reactive oxygen production.. Blood. 2005; 106(7):2543-50. (Biology). View Reference
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