PE Mouse Anti-Mouse IL-27 p28 (IL-30)

BD Pharmingen™ PE Mouse Anti-Mouse IL-27 p28 (IL-30)

Name: PE Mouse Anti-Mouse IL-27 p28 (IL-30)
Brand: PE Mouse Anti-Mouse IL-27 p28 (IL-30)
SKU: 568409

クローン MM27.7B1 (also known as MM27-7B1) (RUO)

Flow cytometric analysis of IL-27 p28 (IL-30) expression by activated RAW264.7 cells. Mouse RAW264.7 cells were cultured with IFN-γ (Cat. No. 554587; 24 h; Left Plot) followed by lipopolysaccharide (LPS, Sigma L3137, 1 µg/ml; additional 24 h). BD GolgiStop™ (Cat. No. 554724) was added for the final 5 h of culture. RAW264.7 cells were cultured with tissue culture medium alone as a control (Right Plot). BD Horizon™ Fixable Viability Stain 510 (FVS510; Cat. No. 564406) was added for the last 7 minutes of culture. Cells were then fixed and permeabilized using BD Cytofix/Cytoperm™ (Cat. No. 554722) and stained with either PE Mouse IgG2a, κ Isotype Control (Cat. No. 553457; dashed line histogram) or PE Mouse Anti-Mouse IL-27 p28 (IL-30) antibody (Cat. No. 568408/568409; solid line histogram) at 0.5 µg/test according to the standard intracellular staining protocol. The fluorescence histograms showing IL-27 p28 (IL-30) expression (or Ig Isotype control staining) by RAW264.7 cells treated with (Left Plot) or without (Right Plot) IFN-γ and LPS were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

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カタログ番号:568409

希望小売価格:¥48,030

単位:EA (1 Each)

容量:

25 µg

数量:

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製品詳細

Brand: BD Pharmingen™

Alternative Name: Il27; IL-27 alpha; Il27a; IL27-A; IL-27-A; IL-27α; IL-27 p28; Il30; IL-30

Reactivity: Mouse (QC Testing)

Isotype: Mouse C57BL/6 IgG2a, κ

Immunogen: Mouse IL-27

Application: Intracellular staining (flow cytometry) (Routinely Tested)

Concentration: 0.2 mg/ml

Entrez Gene ID: 246779

RRID: AB_3684250

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.

推奨アッセイ手順

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
For U.S. patents that may apply, see bd.com/patents.

データシート

Technical Data Sheet (TDS)

関連製品

Stain Buffer (FBS) RUO

サイズ 500 mL カタログ番号 554656

Stain Buffer (BSA) RUO

サイズ 500 mL カタログ番号 554657

Recombinant Mouse IFN-γ RUO

サイズ 10 µg カタログ番号 554587

Protein Transport Inhibitor (Containing Monensin) RUO

サイズ 0.7 mL カタログ番号 554724

Fixation and Permeabilization Solution RUO

サイズ 125 mL カタログ番号 554722

PE Mouse IgG2a, κ Isotype Control RUO

サイズ 0.1 mg カタログ番号 553457

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568409 Rev. 1

抗体の詳細

MM27.7B1

The MM27.7B1 monoclonal antibody specifically binds to the p28 subunit of mouse Interleukin-27 (IL-27 p28) that is also known as IL-27 alpha (IL-27α) or Interleukin-30 (IL-30). IL-27 p28 is a member of the IL-6/IL-12 family of cytokines. The IL-27 p28 subunit binds to the EBI3 (Epstein-Barr virus induced gene 3) protein to form heterodimeric Interleukin-27. IL-27 p28 is produced by macrophages and dendritic cells stimulated by inflammatory cytokines and ligands for Toll-like receptors (TLR). IL-27 binds to and transduces signal through surface IL-27 receptors (IL-27R) that are expressed by B cells and naïve T cells and more highly expressed by NK cells and activated T cells. The IL-27R complex is comprised of gp130 (CD130) and WSX-1 (IL-27R alpha) subunits. IL-27 signals through IL-27R that activate the Jak/STAT signaling pathway resulting in the phosphorylation of Stat1 and Stat3. It acts in a proinflammatory manner to upregulate cellular T-bet transcription factor and surface IL-12R beta2 expression contributing to higher levels of IFN-γ and granzyme B expression. IL-27 exerts anti-inflammatory responses by suppressing the differentiation and proliferation of Th2 and Th17 cells. IL-27 p28 is reportedly produced and secreted independently of EBI3. It may inhibit the actions of IL-17 and other proinflammatory cytokines like IL-6. The MM27/7B1 antibody has been reported to neutralize IL-27 activity in vitro. Routine quality testing of mAb MM27.7B1 is performed via flow cytometry of intracellularly stained IL-27 p28-transfected cells.

568409 Rev. 1

フォーマットの詳細

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

フォーマット: PE

励起源: Yellow-Green 561 nm

最大励起波長: 496 nm, 566 nm

最大蛍光波長: 576 nm

568409 Rev.1

引用＆参考文献

View Development References

Development References (9)

Hall AO, Silver JS, Hunter CA. The immunobiology of IL-27. Adv Immunol. 2012; 115:1-44. (Biology). View Reference
Hibbert L, Pflanz S, De Waal Malefyt R, Kastelein RA. IL-27 and IFN-alpha signal via Stat1 and Stat3 and induce T-Bet and IL-12Rbeta2 in naive T cells. J Interferon Cytokine Res. 2003; 23(9):513-522. (Biology). View Reference
Morishima N, Owaki T, Asakawa M, Kamiya S, Mizuguchi J, Yoshimoto T. Augmentation of effector CD8+ T cell generation with enhanced granzyme B expression by IL-27. J Immunol. 2005; 175(3):1686-1693. (Biology). View Reference
Pflanz S, Timans JC, Cheung J, et al. IL-27, a heterodimeric cytokine composed of EBI3 and p28 protein, induces proliferation of naive CD4(+) T cells. Immunity. 2002; 16(6):779-790. (Biology). View Reference
Stumhofer JS, Hunter CA. Advances in understanding the anti-inflammatory properties of IL-27. Immunol Lett. 2008; 117(2):123-130. (Biology). View Reference
Stumhofer JS, Tait ED, Quinn WJ, 3rd, et al. A role for IL-27p28 as an antagonist of gp130-mediated signaling. Nat Immunol. 2010; 11(12):1119-1126. (Biology). View Reference
Uyttenhove C, Marillier RG, Tacchini-Cottier F, et al. Amine-reactive OVA multimers for auto-vaccination against cytokines and other mediators: perspectives illustrated for GCP-2 in L. major infection. J Leukoc Biol. 2011; 89(6):1001-1007. (Immunogen: Neutralization). View Reference
Yoshimoto T, Okada K, Morishima N, et al. Induction of IgG2a class switching in B cells by IL-27. J Immunol. 2004; 173(4):2479-2485. (Biology). View Reference
Yoshimura T, Takeda A, Hamano S, et al. Two-sided roles of IL-27: induction of Th1 differentiation on naive CD4+ T cells versus suppression of proinflammatory cytokine production including IL-23-induced IL-17 on activated CD4+ T cells partially through STAT3-dependent mechanism. J Immunol. 2006; 177(8):5377-5385. (Biology). View Reference

すべて表示する (9)

568409 Rev. 1

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