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      Purified Mouse Anti-Human P-glycoprotein (CD243)
      Purified Mouse Anti-Human P-glycoprotein (CD243)
      Flow cytometric profile of CD243 expression on PMG-Y cell line. PMG-Y cells were internally transfected and developed to express CD243. They were first stained with either Purified Mouse IgG2b, ĸ Isotype Control (Cat. No. 555740; dashed line histogram) or Purified Mouse Anti-Human P-glycoprotein (CD243) (Cat. No. 557001; solid line histogram), then FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988). Fluorescent histograms were derived from gated events with the forward and side light-scattering characteristics of viable cells. Flow cytometric analysis was performed on a BD FACScan™.
      Purified Mouse Anti-Human P-glycoprotein (CD243)
      Flow cytometric profile of CD243 expression on PMG-Y cell line. PMG-Y cells were internally transfected and developed to express CD243. They were first stained with either Purified Mouse IgG2b, ĸ Isotype Control (Cat. No. 555740; dashed line histogram) or Purified Mouse Anti-Human P-glycoprotein (CD243) (Cat. No. 557001; solid line histogram), then FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988). Fluorescent histograms were derived from gated events with the forward and side light-scattering characteristics of viable cells. Flow cytometric analysis was performed on a BD FACScan™.
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      BD Pharmingen™
      CD243; ABCB1; ABC20; CLCS; GP170; MDR1; P-GP; PGY1
      Human (QC Testing)
      Mouse BALB/c IgG2b, κ
      Human MDR1 Transfected Cell Line
      Flow cytometry (Routinely Tested), Immunoprecipitation (Tested During Development), Western blot (Reported)
      0.5 mg/ml
      AB_396547
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      3. An isotype control should be used at the same concentration as the antibody of interest.
      4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
      5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      557001 Rev. 9
      抗体の詳細
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      17F9

      The 17F9 monoclonal antibody specifically binds to the 170-180 kDa transmembrane glycoprotein (P-glycoprotein), a product of the multidrug resistance-1 (MDR1) gene. This glycoprotein is expressed on MDR positive cells and has been reported to be expressed on many normal tissues, such as adrenal glands and endothelium, in the brain and skin. P-glycoprotein is known to impart drug resistance to cells by pumping many anti-cancer drugs out of the cytoplasm. 17F9 antibody is able to partially block the binding  of UIC2 antibody (another MDR-specific monoclonal antibody).

      557001 Rev. 9
      フォーマットの詳細
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      557001 Rev.9
      引用＆参考文献
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      Development References (3)

      1. Benard J, Bourhis J, Riou G. Clinical significance of multiple drug resistance in human cancers. Anticancer Res. 1990; 10(5A):1297-1302. (Biology). View Reference
      2. Goldstein LJ, Galski H, Fojo A, et al. Expression of a multidrug resistance gene in human cancers. J Natl Cancer Inst. 1989; 81(2):116-124. (Biology). View Reference
      3. Shi T, Wrin J, Reeder J, Liu D, Ring DB. High-affinity monoclonal antibodies against P-glycoprotein. Clin Immunol Immunopathol. 1995; 76(1):44-51. (Biology). View Reference
      557001 Rev. 9

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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