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FITC Goat Anti-Mouse IgG/IgM

FITC Goat Anti-Mouse IgG/IgM

クローン Polyclonal

(RUO)
製品詳細
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BD Pharmingen™
human adsorbed
Mouse (QC Testing)
Goat Ig
Flow cytometry (Routinely Tested)
0.5 mg/ml
AB_396275
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation and Storage

The polyclonal antibody was purified from antiserum by negative adsorption and affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

推奨アッセイ手順

The polyclonal goat anti-mouse IgG + IgM antiserum was purified by immunoaffinity chromatography using antigen coupled to agarose beads. It contains minimal cross-reactivity to human, cow, and horse serum proteins. This reagent is effective as a secondary step using murine monoclonal antibodies having IgG1, IgG2a, IgG2b, IgG3 and IgM isotypes.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
555988 Rev. 1
抗体の詳細
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Gt/Ms

This antibody reacts with the heavy chains IgG and IgM and with light chains common to most mouse immunoglobulins.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

555988 Rev. 1
フォーマットの詳細
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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FITC
Blue 488 nm
494 nm
518 nm
555988 Rev.1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.