BD Pharmingen™ Purified Rat Anti-Mouse Ig, λ1, λ2 & λ3 Light Chain

For the sandwich mouse IgG1, G2a, G2b, G3, IgM, IgA, and IgE ELISA, Biotin Rat Anti-Mouse Ig, λ1, λ2, & λ3 Light Chain (Cat. No. 553433) cocktailed with Biotin Rat Anti-Mouse Ig, κ Light Chain (Cat. No. 559750) is optimal for detection with any of the following anti-mouse Ig isotype-specific mAbs: Purified Rat Anti-Mouse IgG1 (Cat. No. 553440); Purified Rat Anti-Mouse IgG2a (Cat. No. 553387); Purified Rat Anti-Mouse IgG2b (Cat. No. 553392); R40-82 [available by custom order]); Purified Rat Anti-Mouse IgM (Cat. No. 553435); Purified Rat Anti-Mouse IgA (Cat. No. 556969); Purified Rat Anti-Mouse IgE (Cat. No. 553413) for capture, respectively.

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.