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Purified Rat Anti-Mouse Ig, λ1, λ2 & λ3 Light Chain R26-46 RUO

Purified Rat Anti-Mouse Ig, λ1, λ2 & λ3 Light Chain 

Clone  R26-46   (RUO)

  • Brand BD Pharmingen™
  • Alternative Name Igl; Ig λ; Immunoglobulin lambda chain complex; Ig λ1/λ2/λ3
  • Concentration 0.5 mg/ml
  • Isotype Rat IgG2a, κ
  • Reactivity Mouse (QC Testing) 
  • Application ELISA (Routinely Tested) 
  • Immunogen Pooled Mouse Ig
  • Entrez Gene ID 111519 
  • Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
  • Regulatory Status RUO
Product Details Recommended Assay References

Description

The R26-46 antibody reacts specifically with mouse Igs bearing λ1, λ2, or λ3 light chains. It does not react with κ light chain or heavy chain. Detection of surface immunoglobulin on Ig λ chain-secreting hybridoma cells has been demonstrated with R26-46 mAb.

Format
  • Format Purified

Other Formats →

Suggested Companion Products

Biotin Rat Anti-Mouse Ig, κ Light Chain 187.1 RUO
0.5 mg Cat No: 559750

Biotin Rat Anti-Mouse Ig, λ1, λ2, & λ3 Light Chain R26-46 RUO
0.5 mg Cat No: 553433

Purified Rat Anti-Mouse IgG1 A85-1 RUO
0.5 mg Cat No: 553440

Purified Rat Anti-Mouse IgG2a R19-15 RUO
0.5 mg Cat No: 553387

Purified Rat Anti-Mouse IgG2b R12-3 RUO
0.5 mg Cat No: 553392

Purified Rat Anti-Mouse IgM II/41 RUO
0.5 mg Cat No: 553435

Purified Rat Anti-Mouse IgA C10-3 RUO
0.5 mg Cat No: 556969

Purified Rat Anti-Mouse IgE R35-72 RUO
0.5 mg Cat No: 553413

Resources & Tools
 Spectrum Viewer  Download TDS  Regulatory Document Website
Preparation and Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  4. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.


For the sandwich mouse IgG1, G2a, G2b, G3, IgM, IgA, and IgE ELISA, Biotin Rat Anti-Mouse Ig, λ1, λ2, & λ3 Light Chain (Cat. No. 553433) cocktailed with Biotin Rat Anti-Mouse Ig, κ Light Chain (Cat. No. 559750) is optimal for detection with any of the following anti-mouse Ig isotype-specific mAbs: Purified Rat Anti-Mouse IgG1 (Cat. No. 553440); Purified Rat Anti-Mouse IgG2a (Cat. No. 553387); Purified Rat Anti-Mouse IgG2b (Cat. No. 553392); R40-82 [available by custom order]); Purified Rat Anti-Mouse IgM (Cat. No. 553435); Purified Rat Anti-Mouse IgA (Cat. No. 556969); Purified Rat Anti-Mouse IgE (Cat. No. 553413) for capture, respectively.


  1. Li Y, Li H, Weigert M. Autoreactive B cells in the marginal zone that express dual receptors.. J Exp Med. 2002; 195(2):181-8. View reference

  2. Rolink AG, Winkler T, Melchers F, Andersson J. Precursor B cell receptor-dependent B cell proliferation and differentiation does not require the bone marrow or fetal liver environment.. J Exp Med. 2000; 191(1):23-32. View reference

553432 Rev. 12
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