Dear Customer, If you experience any text readability or display issues on our website, please update your web browser to the latest version

BD Transduction Laboratories™ Purified Mouse Anti-Rap1

Name: Purified Mouse Anti-Rap1
Brand: Purified Mouse Anti-Rap1
SKU: 610195

クローン 3/Rap1 (RUO)

Western blot analysis of Rap1 on a Jurkat cell lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the anti- Rap1 antibody.

Immunofluorescence staining of A431 cells.

Western blot analysis of Rap1 on a Jurkat cell lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the anti- Rap1 antibody.

Immunofluorescence staining of A431 cells.

Spectrum Viewer

Protocol Library

Scientific Resources

製品詳細

Brand: BD Transduction Laboratories™

Reactivity: Human (QC Testing), Mouse, Rat, Chicken, Frog (Tested in Development)

Isotype: Mouse IgG1

Immunogen: Human Rap1 aa. 1-184

Application: Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry-formalin (antigen retrieval required), Immunoprecipitation (Tested During Development)

Target Molecular Weight: 21 kDa

Concentration: 250 µg/ml

RRID: AB_397595

Storage Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.

関連製品

HRP Goat Anti-Mouse Ig RUO

サイズ 1 ML カタログ番号 554002

Jurkat Cell Lysate RUO

サイズ 500 µg カタログ番号 611451

FITC Goat Anti-Mouse Ig RUO

サイズ 0.5 mg カタログ番号 554001

610195 Rev. 1

抗体の詳細

3/Rap1

Rap1 is a member of the large Ras superfamily of low molecular weight GTP/GDP binding proteins. Like Ras, the Rap proteins cycle between a GDP-bound inactive form and a GTP-bound active form. Since Ras and Rap have the same amino acid sequence in their putative effector domain (aa. 32-40), it seems likely that they perform either similar or antagonistic functions. Rap1A and Rap1B are highly homologous proteins, differing in only 9 of their 184 amino acids. Overexpression of Rap1A (also known as Krev-1) causes reversion of the phenotype of a Ki-Ras-transformed cell line. In vitro, Rap1 can compete efficiently with p21ras for interaction with Ras-GAP. Though they appear to have similar activities, Rap1 and Ras differ in their cellular localization. Ras is found on the inner surface of the plasma membrane while Rap1 is associated with the Golgi.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610195 Rev. 1

フォーマットの詳細

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

フォーマット: Purified

610195 Rev.1

引用＆参考文献

View product citations for antibody "610195" on CiteAb

Development References (5)

Larson MK, Chen H, Kahn ML, et al. Identification of P2Y12-dependent and -independent mechanisms of glycoprotein VI-mediated Rap1 activation in platelets. Blood. 2003; 101(4):1409-1415. (Biology: Western blot). View Reference
Okada S, Pessin JE. Insulin and epidermal growth factor stimulate a conformational change in Rap1 and dissociation of the CrkII-C3G complex. J Biol Chem. 1997; 272(45):28179-28182. (Biology: Immunoprecipitation, Western blot). View Reference
Wu C, Lai CF, Mobley WC. Nerve growth factor activates persistent Rap1 signaling in endosomes. J Neurosci. 2001; 21(15):5406-5416. (Biology: Immunofluorescence, Western blot). View Reference
Xing L, Ge C, Zeltser R, Maskevitch G, Mayer BJ, Alexandropoulos K. c-Src signaling induced by the adapters Sin and Cas is mediated by Rap1 GTPase. Mol Cell Biol. 2000; 20(19):7363-7377. (Biology: Western blot). View Reference
Yamamoto T, Kaibuchi K, Mizuno T, Hiroyoshi M, Shirataki H, Takai Y. Purification and characterization from bovine brain cytosol of proteins that regulate the GDP/GTP exchange reaction of smg p21s, ras p21-like GTP-binding proteins. J Biol Chem. 1990; 265(27):16626-16634. (Biology). View Reference

すべて表示する (5)

610195 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.