This is a suggested alternate SKU for 551109.

BD Pharmingen™ Purified Mouse Anti-Human Bcl-2 w/Control

Name: Purified Mouse Anti-Human Bcl-2 w/Control
Brand: Purified Mouse Anti-Human Bcl-2 w/Control
SKU: 551107

クローン Bcl-2/100 (also known as 100) (RUO)

Protocol Library

Scientific Resources

Profile of permeabilized lymphocytes analyzed on a FACScan (BDIS, San Jose, CA) (left panel). Cells were stained with anti-human Bcl-2 (clone Bcl-2/100) or a mouse IgG1 isotype (negative) control followed by FITC-conjugated second step.

Formalin-fixed, paraffin-embedded tissue sections stained for Bcl-2 expression using clone Bcl-2/100 and the SA-HRP method. (Left panel) Normal tonsil. (Right panel) Follicular lymphoma. In normal tonsil the mantle zone and intrafollicular region are stained; only occasional Bcl-2 positive cells are seen in the germinal center. In follicular lymphoma, tumor follicles are Bcl-2 positive.

Western blot analysis of Bcl-2 (right panel). Lysate from Jurkat cells was probed with clone Bcl-2/100 at concentrations of 1.0 (lane 1), 0.5 (lane 2), and 0.125 µg/ml (lane 3). Bcl-2 is identified as a band of ~26 kDa.

Protocol Library

Scientific Resources

製品詳細

Brand: BD Pharmingen™

Reactivity: Human (QC Testing)

Application: Western blot (Routinely Tested), Flow cytometry, Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen (Tested During Development)

Regulatory Status: RUO

説明

Bcl-2 is considered to be novel among proto-oncogenes because it blocks apoptosis (programmed cell death) in many cell types. Apoptosis is an active form of cellular suicide that typically requires new RNA and protein synthesis and is associated with distinct morphological changes including cell shrinkage, cytoplasm membrane blebbing, nuclear fragmentation and DNA degradation. Because Bcl-2 blocks apoptosis it may contribute to tumorigenisis by prolonging cell survival, rather than by accelerating the rate of cell proliferation. Human Bcl-2 protein migrates at a molecular weight of ~26 kDa by SDS-PAGE. Bcl-2/100 recognizes a 26 kDa band representing human Bcl-2. Additional minor bands at 27-31 kDa and 18-21 kDa may also be visualized. The 27-31 kDa upper band may represent a larger isoform, whereas the 18-21 kDa lower band may be an internal translation or proteolytic product, therefore, a synthetic peptide corresponding to amino acids 41-54 (GAAPAPGIFSSQPG) of human Bcl-2 was used as immunogen. This peptide sequence is not conserved between human and mouse. Bcl-2/100 does not cross-react with mouse Bcl-2. For detection of mouse Bcl-2 refer to clone 3F11 (Cat. No. 554218), polyclonal rabbit anti-rat/mouse Bcl-2 antiserum (Cat. No. 554087), and polyclonal rabbit anti-mouse Bcl-2 antiserum (Cat. No. 554279).

調製と保管

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

推奨アッセイ手順

Western Blot: Jurkat control lysate [50 µg (1 µg/µl)] is provided as a western blot positive control (Comp. No. 51-16526N; Store lysate at -20°C). Additional control lysate (Cat. No. 611451) is sold separately.

IHC: For paraffin-embedded sections, tissues should be fixed in either Bouin's fixative or formalin.

For immunofluorescent staining and flow cytometry, our directly conjugated formats are recommended, Cat. No. 556535 and 556357.

製品通知

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.

551107 Rev. 5

構成品

説明	数量/容量	Part Number	クローン	アイソタイプ
Purified Mouse Anti-Human Bcl-2	50 µg (1 ea)	51-6511GR	Bcl-2/100	IgG1, κ
Jurkat Cell Lysate	50 µg (1 ea)	51-16526N	N/A	N/A

551107 Rev. 5

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.