FITC Mouse Anti-Pig CD8a
Clone 76-2-11 (RUO)
- Brand BD Pharmingen™
- Concentration 0.5 mg/ml
- Isotype Mouse BALB/c IgG2a, κ
- Reactivity Pig (QC Testing)
Flow cytometry (Routinely Tested)
- Immunogen dd miniature swine thymocytes
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The 76-2-11 (also known as clone PT8) monoclonal antibody specifically binds to an epitope on the CD8α chain, a 35-kDa antigen expressed on thymocytes, peripheral T lymphocytes, and NK cells. The CD8 molecule can exist as a 70 kDa homodimer, composed of α chains, or heterodimer, composed of an α and β chain. Cells which express the CD8αα homodimer display dimmer staining with mAb 76-2-11 than CDαβ-expressing cells. The 76-2-11 mAb does not cross-react with human or bovine cells. Two peripheral CD8+ T-cell populations can be distinguished in the pig: CD8-bright CD4- CTL effectors/precursors and CD8-dull CD4+ T-helper lymphocytes. Pig NK cells express CD8 (dull staining), CD2, MHC class II, LFA-1, and asialo-GM1, but not CD3, CD4, CD5, or CD6. mAb 76-2-11 has been reported to partially inhibit in vitro cytotoxic activity of PBL to allogeneic leukocytes, but not NK-cell-mediated lysis, and to deplete CD8+ T cells in vivo. This clone was clustered as anti-CD8a at the First International Swine CD workshop.
FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.
Suggested Companion Products
|Resources & Tools|
|Spectrum Viewer||Panel Designer||Spectrum Viewer||Download TDS||Regulatory Document Website|
Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.