RY610 Mouse Anti-Human HLA-DR4

BD OptiBuild™ RY610 Mouse Anti-Human HLA-DR4

Name: RY610 Mouse Anti-Human HLA-DR4
Brand: RY610 Mouse Anti-Human HLA-DR4
SKU: 758986

クローン NFLD.D1 (RUO)

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カタログ番号:555511

希望小売価格:¥45,760

単位:EA (1 Each)

容量:

50 µg

数量:

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製品詳細

Brand: BD OptiBuild™

Alternative Name: HLA-DR4; HLA-DRB1*04

Reactivity: Human (Tested in Development)

Isotype: Mouse IgG1, λ

Immunogen: Human HLA-DRB1*0403 Transfected Cells

Application: Flow cytometry (Qualified)

Concentration: 0.2 mg/ml

Entrez Gene ID: 3123

RRID: AB_3691088

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.

推奨アッセイ手順

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
For U.S. patents that may apply, see bd.com/patents.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
Please observe the following precautions: We recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to protect exposure of conjugated reagents, including cells stained with those reagents, to any room illumination. Absorption of visible light can significantly affect the emission spectra and quantum yield of tandem fluorochrome conjugates.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
An isotype control should be used at the same concentration as the antibody of interest.
CF™ is a trademark of Biotium, Inc.
Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.

詳細を表示

データシート

Technical Data Sheet (TDS)

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758986 Rev. 1

抗体の詳細

NFLD.D1

The NFLD.D1 monoclonal antibody is specific for the HLA-DR4 serogroup. This panspecific HLA-DR4 antibody binds to an epitope expressed in the extracellular beta-2 (β2) domain of all HLA-DRB1 beta chain variants encoded by HLA-DRB1*04 alleles. The epitope recognized by the NFLD.D1 antibody depends on both a leucine present at sequence position 180 and a threonine at position 181. HLA-DR antigens are heterodimers comprised of type I transmembrane glycoprotein alpha and beta subunits that contain two extracellular domains, transmembrane regions, and cytoplasmic tails. The ~34 kDa HLA-DR alpha (HLA-DRα) chain is encoded by HLA-DRA whereas the alternative ~28 kDa HLA-DR beta (HLA-DRβ) chains are encoded by one of the four different HLA-DRB loci (HLA-DRB1,3,4, and 5) that are located within the Human Leukocyte Antigen (HLA) Complex of chromosome 6. HLA-DR is variably expressed on antigen-presenting cells (APCs), such as dendritic cells, B lymphocytes, monocytes, macrophages, and thymic epithelial cells as well as by activated T cells, epithelial cells, inflammatory fibroblasts, and tumor cells. Polymorphisms in the HLA-DRβ chains allow for variability in peptide binding and the presentation of self and foreign peptide antigens to CD4+ T lymphocytes that generate and regulate adaptive immune responses. The NFLD.D1 antibody can be especially useful along with other HLA-DRβ allotype-specific antibodies in applications such as immunofluorescence and flow cytometry for analyzing allelic HLA-DRB expression patterns by individual cells within heterogeneous cell populations. Some HLA-DRB1*04 alleles or their aberrant expression patterns have been associated with susceptibility to diseases including rheumatoid arthritis.

758986 Rev. 1

フォーマットの詳細

RY610

The BD Horizon RealYellow™ 610 (RY610) Dye is part of the BD® family of yellow-green dyes. It is a tandem fluorochrome with an excitation maximum (Ex Max) at 557-nm and an emission maximum (Em Max) at 610-nm as measured using an antibody-dye conjugate. Driven by BD® innovation, RY610 can be used on both spectral and conventional cytometers and is designed to be excited by the Yellow-Green laser (561-nm) with minimal excitation by the 488-nm blue laser. For conventional instruments equipped with a Yellow-Green laser (561-nm), RY610 can be used as an alternative to PE-CF594 and we recommend using an optical filter centered near 610-nm (eg, a 610/20-nm bandpass filter). For spectral instruments equipped with a Yellow-Green laser (561-nm), it can be used in conjunction with PE-CF594.

フォーマット: RY610

励起源: Yellow-Green 561 nm

最大励起波長: 557 nm

最大蛍光波長: 610 nm

758986 Rev.1

引用＆参考文献

Development References (3)

Drover S, Karr RW, Fu XT, Marshall WH. Analysis of monoclonal antibodies specific for unique and shared determinants on HLA-DR4 molecules.. Hum Immunol. 1994; 40(1):51-60. (Biology). View Reference
Marshall, W. H., S. Drover, et al. Assessing prognosis in rheumatoid arthritis using monoclonal antibodies and flow cytometry. In: S. Drover. Madrigal A.J., Bencová M., Middleton D., Charron D., Nánási T., ed. Immunogenetics: Advances and Education. Dordrecht: Springer; 1997:87-98.
Patil NS, Pashine A, Belmares MP, et al. Rheumatoid arthritis (RA)-associated HLA-DR alleles form less stable complexes with class II-associated invariant chain peptide than non-RA-associated HLA-DR alleles.. J Immunol. 2001; 167(12):7157-68. (Biology). View Reference

758986 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.