RY610 Mouse Anti-Human CD49c (Integrin α3)

BD OptiBuild™ RY610 Mouse Anti-Human CD49c (Integrin α3)

Name: RY610 Mouse Anti-Human CD49c (Integrin α3)
Brand: RY610 Mouse Anti-Human CD49c (Integrin α3)
SKU: 758553

クローン P1B5 (RUO)

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カタログ番号:758553

希望小売価格:¥78,650

単位:EA (1 Each)

容量:

50 µg

数量:

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製品詳細

Brand: BD OptiBuild™

Alternative Name: ITGA3; integrin alpha 3; alpha 3 subunit of VLA-3 receptor; VLA3a

Reactivity: Human (Tested in Development)

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Human WI-38 VA13 or HT-1080 Cells

Application: Flow cytometry (Qualified)

Concentration: 0.2 mg/ml

Workshop Number: VIII 80337

Entrez Gene ID: 3675

RRID: AB_3690683

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.

推奨アッセイ手順

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
For U.S. patents that may apply, see bd.com/patents.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
Please observe the following precautions: We recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to protect exposure of conjugated reagents, including cells stained with those reagents, to any room illumination. Absorption of visible light can significantly affect the emission spectra and quantum yield of tandem fluorochrome conjugates.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
An isotype control should be used at the same concentration as the antibody of interest.
CF™ is a trademark of Biotium, Inc.
Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.

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データシート

Technical Data Sheet (TDS)

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758553 Rev. 1

抗体の詳細

P1B5

The P1B5 monoclonal antibody specifically recognizes Integrin alpha 3 (Integrin α3) which is also known as CD49c (CD49 antigen-like family member C) or VLA-3 subunit alpha (VLA3a or VLA-3α). CD49c (Integrin α3) is a 150 kDa type I transmembrane glycoprotein that is encoded by ITGA3 (Integrin subunit alpha 3) which belongs to the Integrin alpha chain family. CD49c (Integrin α3) non-covalently associates with integrin β1 (CD29) to form heterodimeric Integrin α3β1 (CD49c/CD29 or VLA-3 complex). CD49c is primarily expressed on endothelial and epithelial cells (basal epidermal layers). It is weakly expressed on peripheral blood leucocytes, including T cells, B cells, and monocytes, but is not expressed on platelets. The CD49c/CD29 complex serves as an adhesive receptor for extracellular membrane components including fibronectin, collagen, laminin-1, laminin V, and entactin.

758553 Rev. 1

フォーマットの詳細

RY610

The BD Horizon RealYellow™ 610 (RY610) Dye is part of the BD® family of yellow-green dyes. It is a tandem fluorochrome with an excitation maximum (Ex Max) at 557-nm and an emission maximum (Em Max) at 610-nm as measured using an antibody-dye conjugate. Driven by BD® innovation, RY610 can be used on both spectral and conventional cytometers and is designed to be excited by the Yellow-Green laser (561-nm) with minimal excitation by the 488-nm blue laser. For conventional instruments equipped with a Yellow-Green laser (561-nm), RY610 can be used as an alternative to PE-CF594 and we recommend using an optical filter centered near 610-nm (eg, a 610/20-nm bandpass filter). For spectral instruments equipped with a Yellow-Green laser (561-nm), it can be used in conjunction with PE-CF594.

フォーマット: RY610

励起源: Yellow-Green 561 nm

最大励起波長: 557 nm

最大蛍光波長: 610 nm

758553 Rev.1

引用＆参考文献

Development References (4)

Halasz P, Fleming FE, Coulson BS. Evaluation of specificity and effects of monoclonal antibodies submitted to the Eighth Human Leucocyte Differentiation Antigen Workshop on rotavirus-cell attachment and entry. Cell Immunol. 2005; 236(1-2):179-187. (Clone-specific: Flow cytometry). View Reference
Melssen MM, Olson W, Wages NA, et al. Formation and phenotypic characterization of CD49a, CD49b and CD103 expressing CD8 T cell populations in human metastatic melanoma.. Oncoimmunology. 7(10):e1490855. (Immunogen: Blocking, Immunoprecipitation). View Reference
Wayner EA, Gil SG, Murphy GF, Wilke MS, Carter WG. Epiligrin, a component of epithelial basement membranes, is an adhesive ligand for alpha 3 beta 1 positive T lymphocytes.. J Cell Biol. 1993; 121(5):1141-52. (Clone-specific: Blocking, Flow cytometry, Immunoprecipitation). View Reference
Zola H. CD49c. In: Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007:124.

すべて表示する (4)

758553 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.