BV711 Mouse Anti-Human CD150

BD OptiBuild™ BV711 Mouse Anti-Human CD150

Name: BV711 Mouse Anti-Human CD150
Brand: BV711 Mouse Anti-Human CD150
SKU: 744347

クローン A12 (RUO)

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カタログ番号:744347

希望小売価格:¥66,550

単位:EA (1 Each)

容量:

50 µg

数量:

見積に追加

見積リストに保存バルク試薬見積の依頼

製品詳細

Brand: BD OptiBuild™

Alternative Name: SLAMF1; Signaling lymphocytic activation molecule; SLAM; SLAF1; IPO-3

Reactivity: Human (Tested in Development)

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Activated Human Peripheral Blood Mononuclear Cells

Application: Flow cytometry (Qualified)

Concentration: 0.2 mg/ml

RRID: AB_2871579

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV711 under optimal conditions that minimize unconjugated dye and antibody.

推奨アッセイ手順

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

データシート

Technical Data Sheet (TDS)

744347 Rev. 1

抗体の詳細

A12

The A12 monoclonal antibody specifically binds to CD150, a single chain type I transmembrane glycoprotein of approximately 75-95 kDa. CD150 is encoded by the SLAMF1 gene and is also known as a signaling lymphocyte activation molecule (SLAM), or IPO-3. It is expressed on T cells, B cells, thymocytes, germinal center and dendritic cells, but not on monocytes. Expression of CD150 is upregulated on T, B and dendritic cells after activation via T-cell and B-cell costimulatory molecules. It has been reported that CD150 plays a role in signal transduction.

The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm. BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor® 700-like dyes (eg, 712/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor® 700 and PerCP-Cy5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination.

744347 Rev. 1

フォーマットの詳細

BV711

The BD Horizon Brilliant Violet™ 711 (BV711) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This tandem fluorochrome is comprised of a BV421 donor with an excitation maximum (Ex Max) of 407-nm and an acceptor dye with an emission maximum (Em Max) at 713-nm. BV711, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 710-nm (e.g., a 712/20-nm bandpass filter). The acceptor dye can be excited by the Red (628–640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

フォーマット: BV711

最大励起波長: 407 nm

最大蛍光波長: 713 nm

744347 Rev.1

引用＆参考文献

No Citations Are Available for this Product

744347 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.