BUV395 Mouse Anti-Human CD125

BD OptiBuild™ BUV395 Mouse Anti-Human CD125

Name: BUV395 Mouse Anti-Human CD125
Brand: BUV395 Mouse Anti-Human CD125
SKU: 743933

クローン A14 (RUO)

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カタログ番号:743933

希望小売価格:¥78,650

単位:EA (1 Each)

容量:

50 µg

数量:

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見積リストに保存バルク試薬見積の依頼

製品詳細

Brand: BD OptiBuild™

Alternative Name: CD125; IL-5Rα; IL5RA; IL5R

Reactivity: Human (Tested in Development)

Isotype: Mouse IgG1, κ

Immunogen: IL-5Rα-transfected cells

Application: Flow cytometry (Qualified)

Concentration: 0.2 mg/ml

RRID: AB_2871554

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BUV395 under optimal conditions that minimize unconjugated dye and antibody.

推奨アッセイ手順

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

This antibody was developed for use in flow cytometry.
The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
Researchers should determine the optimal concentration of this reagent for their individual applications.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
BD Horizon Brilliant Ultraviolet 395 is covered by one or more of the following US patents: 8,158,444; 8,575,303; 8,354,239.

データシート

Technical Data Sheet (TDS)

関連製品

Human BD Fc Block™ RUO

サイズ 50 µg カタログ番号 564219

Stain Buffer (FBS) RUO

サイズ 500 mL カタログ番号 554656

Stain Buffer (BSA) RUO

サイズ 500 mL カタログ番号 554657

Brilliant Stain Buffer RUO

サイズ 100 Tests カタログ番号 563794

Lysing Buffer RUO

サイズ 100 mL カタログ番号 555899

Lysing Solution 10X Concentrate RUO (GMP)

サイズ 100 mL カタログ番号 349202

詳細を表示

743933 Rev. 1

抗体の詳細

A14

The A14 monoclonal antibody specifically recognizes the α chain of the Interleukin-5 Receptor (IL-5Rα), which is also known as CD125. IL-5Rα is a 55-60 kDa type I transmembrane glycoprotein that is expressed on B1 B cells, eosinophils, basophils and mast cells. The IL-5Rα chain associates with CD131 (Common β-chain) which serves as the signaling common β-chain subunit of the IL-5R complex as well as for the Interleukin-3 (IL-3R) and GM-CSF receptor (GM-CSFR) complexes. IL-5 promotes the growth and differentiation of eosinophil precursors and activates mature eosinophils. Clone A14 was developed using IL-5Rα-transfected cells as immunogen. This antibody binding can be blocked by IL-5.

The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry. With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter.

743933 Rev. 1

フォーマットの詳細

BUV395

The BD Horizon Brilliant™ Ultraviolet 395 (BUV395) Dye is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This base dye is a polymer fluorochrome with an excitation maximum (Ex Max) of 348-nm and an emission maximum (Em Max) at 395-nm. Driven by BD innovation, BUV395 is designed to be excited by the ultraviolet laser (355-nm) and detected using an optical filter centered near 380-nm (e.g., 379/28-nm bandpass filter). BUV395 is the ideal dye when using only one detector on the ultraviolet laser as it spills into no other detectors and no other fluors spill into it. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

フォーマット: BUV395

励起源: Ultraviolet 355 nm

最大励起波長: 348 nm

最大蛍光波長: 395 nm

743933 Rev.1

引用＆参考文献

View Development References

Development References (4)

Blom M, Tool AT, Kok PT, Koenderman L, Roos D, Verhoeven AJ. Granulocyte-macrophage colony-stimulating factor, interleukin-3 (IL-3), and IL-5 greatly enhance the interaction of human eosinophils with opsonized particles by changing the affinity of complement receptor type 3. Blood. 1994; 83(10):2978-2984. (Biology). View Reference
Hellman C, Lönnkvist K, Hedlin G, Halldén G, Lundahl J. Down-regulated IL-5 receptor expression on peripheral blood eosinophils from budesonide-treated children with asthma.. Allergy. 2002; 57(4):323-8. (Clone-specific: Flow cytometry). View Reference
Ingley E, Young IG. Characterization of a receptor for interleukin-5 on human eosinophils and the myeloid leukemia line HL-60. Blood. 1991; 78(2):339-344. (Biology). View Reference
Stomski FC, Woodcock JM, Zacharakis B, Bagley CJ, Sun Q, Lopez AF. Identification of a Cys motif in the common beta chain of the interleukin 3, granulocyte-macrophage colony-stimulating factor, and interleukin 5 receptors essential for disulfide-linked receptor heterodimerization and activation of all three receptors.. J Biol Chem. 1998; 273(2):1192-9. (Immunogen: Immunoprecipitation). View Reference

すべて表示する (4)

743933 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.