RY586 Rabbit Anti-Human PKM

BD Horizon™ RY586 Rabbit Anti-Human PKM

Name: RY586 Rabbit Anti-Human PKM
Brand: RY586 Rabbit Anti-Human PKM
SKU: 571525

クローン EPR10138 (B) (RUO)

Flow cytometric analysis of PKM expression in HEK293T cells. Cells from the Human HEK293T (Embryonic Kidney, ATCC® CRL-3216 ™) cell line were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050). The cells were then stained with BD Horizon™ RY586 Rabbit IgG Isotype Control (Cat. No. 571528; dashed line histogram) or BD Horizon™ RY586 Rabbit Anti-Human PKM antibody (Cat. No. 571525; solid line histogram) at 0.25 µg/test. The fluorescence histogram showing PKM expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.

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カタログ番号:553331

希望小売価格:¥56,980

単位:EA (1 Each)

容量:

50 µg

数量:

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製品詳細

Brand: BD Horizon™

Alternative Name: CTHBP; OIP3; OPA-interacting protein 3; PK, muscle type; PKM; PKM2

Reactivity: Human (QC Testing)

Isotype: Rabbit IgG

Immunogen: Human PKM (aa 50-150)

Application: Intracellular staining (flow cytometry) (Routinely Tested)

Concentration: 0.2 mg/ml

Entrez Gene ID: 5315

RRID: AB_3686574

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

推奨アッセイ手順

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
An isotype control should be used at the same concentration as the antibody of interest.
CF™ is a trademark of Biotium, Inc.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
This rabbit monoclonal (RabMabTM) product is, in part, provided under an intellectual property license from Abcam. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product for buyer’s internal research use only (whether the buyer is an academic or for-profit entity) and buyer shall not be permitted to reverse engineer or otherwise perform any compositional, structural, functional or other analyses directed to learning the methodology, formulae, sequences, process, make-up or production of this product (or any portion thereof). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. If you, as the buyer, do not agree to these conditions, please leave the product unopened and return the contents of this package to BD Biosciences, 10975 Torreyana Road, San Diego, CA 92121. For information on purchasing a license to this product for purposes other than research, contact Abcam at partnerships@abcam.com.
For U.S. patents that may apply, see bd.com/patents.

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データシート

Technical Data Sheet (TDS)

関連製品

Stain Buffer (FBS) RUO

サイズ 500 mL カタログ番号 554656

Stain Buffer (BSA) RUO

サイズ 500 mL カタログ番号 554657

Fixation Buffer RUO

サイズ 100 mL カタログ番号 554655

Perm Buffer III RUO

サイズ 125 mL カタログ番号 558050

RY586 Rabbit IgG Isotype Control RUO

サイズ 25 µg カタログ番号 571528

571525 Rev. 1

抗体の詳細

EPR10138 (B)

The EPR10138 (B) recombinant monoclonal antibody specifically recognizes Pyruvate kinase (PKM), which is also known as Cytosolic thyroid hormone-binding protein (CTHBP) or OPA-interacting protein 3 (OIP3). PKM is a cytosolic enzyme that catalyzes the irreversible conversion of phosphoenolpyruvate and ADP to ATP and pyruvic acid in the last step of glycolysis. PKM has two isoforms, PKM1 and PKM2, which represent two splice variants of PKM. PKM1 is found in tissues with high catabolic demand, including non-proliferative tissues and muscle; the embryonic isoform, PKM2, is expressed in cancer, where it plays a central role in caspase-independent cell death of tumor cells.

571525 Rev. 1

フォーマットの詳細

RY586

The BD Horizon RealYellow™ 586 (RY586) Dye is part of the BD family of yellow-green dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 565-nm and an emission maximum (Em Max) at 586-nm. Driven by BD innovation, RY586 can be used on both spectral and conventional cytometers and is designed to be excited by the Yellow-Green laser (561-nm) with minimal excitation by the 488-nm Blue laser. For conventional instruments equipped with a Yellow-Green laser (561-nm), RY586 can be used as an alternative to PE and we recommend using an optical filter centered near 586-nm (eg, a 586/15-nm bandpass filter). For spectral instruments equipped with a Yellow-Green laser (561-nm), it can be used in conjunction with PE. Compared to PE, RY586 is similar in brightness, minimal spillover into Blue detectors, and increased spillover into the 610/20-nm (PE-CF594) detector. Please ensure that your instrument configuration (lasers and optical filters) is appropriate for this dye.

フォーマット: RY586

励起源: Yellow-Green 561 nm

最大励起波長: 564 nm

最大蛍光波長: 586 nm

571525 Rev.1

引用＆参考文献

Development References (4)

Fan XG, Pei SY, Zhou D, et al. Melittin ameliorates inflammation in mouse acute liver failure via inhibition of PKM2-mediated Warburg effect.. Acta Pharmacol Sin. 2021; 42(8):1256-1266. (Clone-specific: Immunofluorescence, Western blot). View Reference
Israelsen WJ, Vander Heiden MG. Pyruvate kinase: Function, regulation and role in cancer.. Semin Cell Dev Biol. 2015; 43:43-51. (Biology). View Reference
Steták A, Veress R, Ovádi J, Csermely P, Kéri G, Ullrich A. Nuclear translocation of the tumor marker pyruvate kinase M2 induces programmed cell death.. Cancer Res. 2007; 67(4):1602-8. (Biology). View Reference
Wang C, He C, Lu S, et al. Autophagy activated by silibinin contributes to glioma cell death via induction of oxidative stress-mediated BNIP3-dependent nuclear translocation of AIF.. Cell Death Dis. 2020; 11(8):630. (Clone-specific: Western blot). View Reference

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571525 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.