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BD Rhapsody™ ATAC-Seq Tagmentation and Supplemental Reagents Kit






Regulatory Statusの凡例
Becton, Dickinson and Companyの書面による明示的な許諾を得た使用以外での製品の使用は固く禁じられています。
説明
(next page)
Description
The BD Rhapsody™ ATAC-Seq Tagmentation and Supplemental Reagents Kit provides the necessary reagents to support bulk in-situ tagmentation of single nuclei isolated from the sample(s) of interest for the preparation of single cell ATAC-Seq libraries with the BD Rhapsody™ Single-Cell Analysis system. Up to 8 samples can be prepared using this kit prior to single nuclei capture with the BD Rhapsody™ Single-Cell Analysis system. Tagmentase in the kit is a custom-made, hyperactive variant of the prokaryotic Tn5 transposase that enables simultaneous fragmentation of open chromatin regions in the isolated nuclei and insertion of sequencing adapters at the cleaved sites. The inserted adapters permit amplification of the transposed fragments, using either the BD Rhapsody™ ATAC-Seq Amplification Kit or the BD Rhapsody™ Multiomic ATAC-Seq Amplification Kit (sold separately), and sequencing of these regions according to the recommendations provided in the library preparation protocols. The data acquired from the sequenced libraries offer genome-wide maps of chromatin accessibility that delineate the location of active regulatory elements including promoters, enhancers, and transcription factor binding sites at the single cell level. Prior to using the BD Rhapsody™ ATAC-Seq Tagmentation and Supplemental Reagents Kit, the nuclei isolation and preparation steps should be performed using protocols recommended elsewhere for the specific cell type(s) of interest. Following the tagmentation step, users will proceed to the single nuclei capture step on the BD Rhapsody™ Single-Cell Analysis system or the BD Rhapsody™ HT Xpress system using one of the protocols listed in the Recommended Assay Procedure section. In addition to the reagents required for the tagmentation step, this kit also provides a splint oligo and accompanying buffers for the generation of a splint-bead duplex that would serve as the binding site for the transposed fragments upon capture from the loaded nuclei.
推奨アッセイ手順
Prior to adding the Tagmentation Buffer to the tagmentation mix, 100% Dimethylformamide (DMF) must be added to the thawed buffer according to the instructions in the library preparation protocols. For detailed instructions on tagmentation step and preparation of standalone ATAC-Seq libraries, refer to Single-Cell ATAC Library Preparation Protocol (Doc ID 23-24473).
For detailed instructions on tagmentation step and preparation of multiomic ATAC-Seq and whole transcriptome analysis libraries, refer to Single-Cell ATAC and mRNA Whole Transcriptome Analysis (WTA) Library Preparation Protocol (Doc ID 23-24474).
For detailed instructions on how to use the BD Rhapsody™ Single-Cell Analysis system, refer to the BD Rhapsody™ HT Xpress Single-Cell Analysis System Instrument User Guide (Doc ID 23-24257) or the BD Rhapsody™ HT Xpress System Instrument User Guide for Scanner-free Workflow (Doc ID 23-24256).
For sample preparation prior to the ATAC-Seq assay, refer to BD Rhapsody™ System Preparing Single Cell Suspensions Protocol (Doc ID 23-24126). For information on the bioinformatic pipeline for the ATAC-Seq assay, refer to BD® Single-Cell Multiomics Bioinformatics Handbook (Doc ID 54169).
For safety information, see the BD Rhapsody ™ Single-Cell Analysis System Instrument User Guide (Doc ID 23-24257).
Use standard laboratory safety protocols. Read and understand the safety data sheets (SDSs) before handling chemicals. To obtain SDSs, go to regdocs.bd.com or contact BD Biosciences technical support at scomix@bdscomix.bd.com.
Warning: All biological specimens and materials coming into contact with them are considered biohazardous. Handle as if capable of transmitting infection and dispose of with proper precautions in accordance with federal, state, and local regulations. Never pipette by mouth. Wear suitable protective clothing, eyewear, and gloves.
Warning: 571201 ATAC-Seq Tagmentation Kit, Kit component 51-9023085 Digitonin.
Digitonin contains 2 wt.% Digitonin, CAS number 11024-24-1, EC number 234-255-6, and 98 wt.% Dimethyl sulfoxide, CAS number 67-68-5, EC number 200-664-3. These reagents are classified as hazardous according to the Globally Harmonized System of Classification and Labelling of Chemicals (GHS).
Warning
Combustible liquid.
Harmful in contact with skin.
May cause damage to organs through prolonged or repeated exposure.
Prevention
Keep away from open flames/hot surfaces. - No smoking.
Do not breathe dust/fume/gas/mist/vapors/spray.
Wear protective gloves/protective clothing/eye protection/face protection.
Response
IF ON SKIN: Wash with plenty of soap and water.
Call a POISON CENTER/doctor if you feel unwell.
Get medical advice/attention if you feel unwell.
Take off contaminated clothing and wash it before reuse.
In case of fire: Use water spray to extinguish.
In case of fire: Use carbon dioxide to extinguish.
Storage
Store in a well-ventilated place. Keep cool.
Disposal
Dispose of contents/container to an approved facility in accordance with local, regional, national, and international regulations.
Warning: 571201 ATAC-Seq Tagmentation Kit, Kit component 51-9022689 Proteinase K
Danger: Proteinase K contains 2.53 wt.% Proteinase, Tritirachium album serine, CAS number 39450-01-6, EC number 254-457-8. These reagents are classified as hazardous according to the Globally Harmonized System of Classification and Labelling of Chemicals (GHS).
Danger
May cause allergy or asthma symptoms or breathing difficulties if inhaled.
Prevention
Avoid breathing dust/fume/gas/mist/vapors/spray.
[In case of inadequate ventilation] wear respiratory protection.
Response
IF INHALED: Remove person to fresh air and keep comfortable for breathing.
If experiencing respiratory symptoms: Call a POISON CENTER or doctor/ physician.
Disposal
Dispose of contents/container to an approved facility in accordance with local, regional, national, and international regulations.
製品通知
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- For U.S. patents that may apply, see bd.com/patents.
説明 | 数量/容量 | Part Number | EntrezGene ID |
---|---|---|---|
N/A | 1.0 | N/A | N/A |
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.