PE Mouse Anti-Human TCR Vα24

BD Pharmingen™ PE Mouse Anti-Human TCR Vα24

Name: PE Mouse Anti-Human TCR Vα24
Brand: PE Mouse Anti-Human TCR Vα24
SKU: 570527

クローン C15 (RUO)

Two-color flow cytometric analysis of TCR Vα24 expression on Human peripheral blood T lymphocytes. Human peripheral blood cells were stained with APC Mouse Anti-Human CD3 antibody (Cat. No. 561811) and with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Plot) or BD Pharmingen™ PE Mouse Anti-Human TCR Vα24 antibody (Cat. No. 570526/570527; Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The bivariate pseudocolor density plot showing the correlated expression of TCR Vα24 (or Ig Isotype control staining) versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD FACSCelesta™ Cell Analyzer System and FlowJo™ software.

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カタログ番号:555423

希望小売価格:¥41,360

単位:EA (1 Each)

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製品詳細

Brand: BD Pharmingen™

Alternative Name: TCR Va24; TCR Valpha24; TCR Vα24; TCRAV24S1; TCRV alpha 24; TRAV24

Reactivity: Human (QC Testing)

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Human T-cell clone

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 5 µl/test

Entrez Gene ID: 28659

RRID: AB_3685815

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.

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BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
An isotype control should be used at the same concentration as the antibody of interest.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
For U.S. patents that may apply, see bd.com/patents.

データシート

Technical Data Sheet (TDS)

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BD Horizon™ BV421 Rat Anti-Mouse GM-CSF(MP1-22E9) RUO

カタログ番号 555423

570527 Rev. 1

抗体の詳細

C15

The C15 monoclonal antibody specifically recognizes the variable alpha 24 region of the α subunit of the human αβ T cell receptor for antigen (TCR Vα24). TCR Vα24 combines with joining region 18 (Jα18) to form Vα24-Jα18 (Arden nomenclature, or TRAV10-TRAJ18 in the IMGT nomenclature). TCR Vα24 can pair with different TCR Vβ region containing TCR β chains. The paired expression of TCR Vα24 with semivariant TCR Vβ11 identifies CD3+TCR αβ+ positive thymocytes and from ~ 0.01-0.92% of peripheral blood CD3+ TCR αβ+ T cells known as invariant NKT (iNKT) cells or Type I NKT cells. iNKT cells can be divided into three subpopulations which are either CD4+, CD8+, or CD4-CD8- double negative. Due to their innate-like responsiveness, tissue resident iNKT cells are capable of rapid effector responses to microbial or self-lipid antigens presented by the cell surface MHC class Ib molecule, CD1d. iNKT cells recognize and respond to α-galactosylceramide (α-GalCer) and various self-lipids such as sphingolipids and diacylglycerols and microbial lipids presented by CD1d. iNKT cells can produce high levels of cytokines, including IL-4 and IFN-γ, and act as cytotoxic T cells against CD1d+ target cells following stimulation. The C15 antibody is useful for multiparameter analyses designed to study the nature of TCR Vα24-positive T cells and T cell clones. When combined with other TCR V region-specific antibodies, C15 is helpful for characterizing TCR Vα repertoires expressed by T cell populations collected from blood, tissues or other sources in health and disease models including inflammation, infectious diseases, autoimmunity, and tumors. The C15 antibody reportedly stimulates the proliferation of TCR Vα24-positive T cells.

570527 Rev. 1

フォーマットの詳細

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

フォーマット: PE

励起源: Yellow-Green 561 nm

最大励起波長: 496 nm, 566 nm

最大蛍光波長: 576 nm

570527 Rev.1

引用＆参考文献

Development References (10)

Arden B, Clark SP, Kabelitz D, Mak TW. Human T-cell receptor variable gene segment families.. Immunogenetics. 1995; 42(6):455-500. (Biology). View Reference
Dellabona P, Padovan E, Casorati G, Brockhaus M, Lanzavecchia A. An invariant V alpha 24-J alpha Q/V beta 11 T cell receptor is expressed in all individuals by clonally expanded CD4-8- T cells.. J Exp Med. 1994; 180(3):1171-6. (Immunogen: Flow cytometry, Fluorescence activated cell sorting). View Reference
Elliott JI. Selection of dual Valpha T cells.. Eur J Immunol. 1998; 28(7):2115-23. (Clone-specific: Flow cytometry). View Reference
Godfrey DI, Uldrich AP, McCluskey J, Rossjohn J, Moody DB. The burgeoning family of unconventional T cells.. Nat Immunol. 2015; 16(11):1114-23. (Biology). View Reference
Kent SC, Hafler DA, Strominger JL, Wilson SB. Noncanonical Valpha24JalphaQ T cells with conservative alpha chain CDR3 region amino acid substitutions are restricted by CD1d.. Hum Immunol. 1999; 60(11):1080-9. (Clone-specific: Flow cytometry). View Reference
Montoya CJ, Pollard D, Martinson J, et al. Characterization of human invariant natural killer T subsets in health and disease using a novel invariant natural killer T cell-clonotypic monoclonal antibody, 6B11.. Immunology. 2007; 122(1):1-14. (Clone-specific: Flow cytometry). View Reference
Padovan E, Casorati G, Dellabona P, Meyer S, Brockhaus M, Lanzavecchia A. Expression of two T cell receptor alpha chains: dual receptor T cells.. Science. 1993; 262(5132):422-4. (Immunogen: Flow cytometry, Fluorescence activated cell sorting). View Reference
Prell C, Konstantopoulos N, Heinzelmann B, et al. Frequency of Valpha24+CD161+ natural killer T cells and invariant TCRAV24-AJ18 transcripts in atopic and non-atopic individuals.. Immunobiology. 2003; 208(4):367-80. (Clone-specific: Flow cytometry). View Reference
Scaviner D, Lefranc MP. The human T cell receptor alpha variable (TRAV) genes.. Exp Clin Immunogenet. 2000; 17(2):83-96. (Biology). View Reference
Vyth-Dreese FA, Sein J, van de Kasteele W, et al. Lack of anti-tumour reactivity despite enhanced numbers of circulating natural killer T cells in two patients with metastatic renal cell carcinoma.. Clin Exp Immunol. 2010; 162(3):447-59. (Clone-specific: Flow cytometry). View Reference

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570527 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

BD Pharmingen™ PE Mouse Anti-Human TCR Vα24

クローン C15 (RUO)

Preparation and Storage

推奨アッセイ手順

Product Notices

関連製品

最近閲覧済み

Development References (10)