PE-CF594 Rat Anti-Mouse MerTK (Mer)

BD Horizon™ PE-CF594 Rat Anti-Mouse MerTK (Mer)

Name: PE-CF594 Rat Anti-Mouse MerTK (Mer)
Brand: PE-CF594 Rat Anti-Mouse MerTK (Mer)
SKU: 570326

クローン BB14-16 (RUO)

Multiparameter flow cytometric analysis of MerTK (Mer) expression on viable normal Mouse peritoneal cavity cells (PEC). PEC from C57BL/6 Mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553142]. The cells were then stained with Alexa Fluor™ 647 Rat Anti-Mouse F4/80 antibody (Cat. No. 565853) and with either BD Horizon™ PE-CF594 Rat IgG1, κ Isotype Control (Cat. No. 562309; Left Plot) or BD Horizon™ PE-CF594 Rat Anti-Mouse MerTK (Mer) antibody (Cat. No. 570326/570402; Right Plot) at 0.25 µg/test. DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of MerTK (Mer) [or Ig Isotype control staining] versus F4/80 was derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) PEC. Flow cytometry and data analysis were performed using a LSR Fortessa X-20 Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.

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カタログ番号:570326

単位:EA (1 Each)

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製品詳細

Brand: BD Horizon™

Alternative Name: Mer; proto-oncogene c-Mer; Mertk; Eyk; Nyk

Reactivity: Mouse (QC Testing)

Isotype: Rat IgG1, κ

Immunogen: Recombinant Mouse MerTK Protein

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

Entrez Gene ID: 17289

RRID: AB_3685666

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

推奨アッセイ手順

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Please observe the following precautions: We recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to protect exposure of conjugated reagents, including cells stained with those reagents, to any room illumination. Absorption of visible light can significantly affect the emission spectra and quantum yield of tandem fluorochrome conjugates.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Texas Red is a registered trademark of Molecular Probes, Inc., Eugene, OR.
CF™ is a trademark of Biotium, Inc.
When excited by the yellow-green (561-nm) laser, the fluorescence may be brighter than when excited by the blue (488-nm) laser.
Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using multi-laser cytometers, which may directly excite both PE and CF™594.
This product is provided under an Agreement between BIOTIUM and BD Biosciences. The manufacture, use, sale, offer for sale, or import of this product is subject to one or more patents or pending applications owned or licensed by Biotium, Inc. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
For U.S. patents that may apply, see bd.com/patents.

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データシート

Technical Data Sheet (TDS)

関連製品

Stain Buffer (FBS) RUO

サイズ 500 mL カタログ番号 554656

Stain Buffer (BSA) RUO

サイズ 500 mL カタログ番号 554657

MonoBlock™ Leukocyte Staining Buffer RUO

サイズ 500 Tests カタログ番号 570002

PE-CF594 Rat IgG1, κ Isotype Control RUO

サイズ 0.1 mg カタログ番号 562309

Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO

サイズ 0.5 mg カタログ番号 553142

DAPI Solution RUO

サイズ 1 mg カタログ番号 564907

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570326 Rev. 1

抗体の詳細

BB14-16

The BB14-16 monoclonal antibody specifically recognizes the Mer tyrosine kinase (MerTK). MerTK is a single-pass type I transmembrane glycoprotein that is encoded by Mertk (c-mer proto-oncogene tyrosine kinase) which is also known as Mer. MerTK (Mer) is comprised of an extracellular region with two immunoglobulin (Ig)-like domains and two fibronectin type III (FNIII) domains, a transmembrane segment, and a conserved intracellular tyrosine kinase domain. MerTK (Mer) belongs to the TAM subfamily of receptor tyrosine kinases (RTK) which includes Axl and Tyro-3. MerTK (Mer) is variably expressed by multiple cell types including monocytes, macrophages, dendritic cells, NK cells, platelets, and epithelial cells such as retinal pigment epithelial cells. Ligand binding leads to receptor dimerization, and autophosphorylation of tyrosine residues within the cytoplasmic MerTK (Mer) domain. This activates downstream signaling pathways that control cellular adhesion, aggregation, phagocytosis/efferocytosis, proliferation, survival, and migration. MerTK (Mer) ligands include Gas6 and Protein S that in turn may be bound to phosphatidylserine expressed on the outer plasma membrane of cells undergoing apoptosis. This promotes the phagocytosis of apoptotic cells. Through these activities, MerTK (Mer) plays major roles in the development and regulation of hematopoiesis and immunity. Abnormal cellular expression of MerTK (Mer) has been associated with some autoimmune diseases and various cancers.

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フォーマットの詳細

PE-CF594

BD Horizon™ PE-CF594 dye is a part of the BD PE family of dyes. This tandem fluorochrome is comprised of a R-Phycoerythrin (PE) donor that has excitation maxima (Ex Max) of 496-nm and 566-nm and an acceptor dye with an emission maximum (Em Max) at 615-nm. PE-CF594, driven by BD innovation, is designed to be excited by the blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and detected using an optical filter centered near 615 nm (e.g., a 610/20-nm bandpass filter). The donor dye can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and the acceptor dye can be excited by the green (532-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

フォーマット: PE-CF594

励起源: Yellow-Green 561 nm

最大励起波長: 496 nm, 566 nm

最大蛍光波長: 615 nm

570326 Rev.1

引用＆参考文献

Development References (3)

Dransfield I, Zagórska A, Lew ED, Michail K, Lemke G. Mer receptor tyrosine kinase mediates both tethering and phagocytosis of apoptotic cells.. Cell Death Dis. 2015; 6:e1646. (Biology). View Reference
Gould WR, Baxi SM, Schroeder R, et al. Gas6 receptors Axl, Sky and Mer enhance platelet activation and regulate thrombotic responses.. J Thromb Haemost. 2005; 3(4):733-41. (Biology). View Reference
Seitz HM, Camenisch TD, Lemke G, Earp HS, Matsushima GK. Macrophages and dendritic cells use different Axl/Mertk/Tyro3 receptors in clearance of apoptotic cells.. J Immunol. 2007; 178(9):5635-42. (Biology). View Reference

570326 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.