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Preparation and Storage
推奨アッセイ手順
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- For U.S. patents that may apply, see bd.com/patents.
関連製品
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The BB14-16 monoclonal antibody specifically recognizes the Mer tyrosine kinase (MerTK). MerTK is a single-pass type I transmembrane glycoprotein that is encoded by Mertk (c-mer proto-oncogene tyrosine kinase) which is also known as Mer. MerTK (Mer) is comprised of an extracellular region with two immunoglobulin (Ig)-like domains and two fibronectin type III (FNIII) domains, a transmembrane segment, and a conserved intracellular tyrosine kinase domain. MerTK (Mer) belongs to the TAM subfamily of receptor tyrosine kinases (RTK) which includes Axl and Tyro-3. MerTK (Mer) is variably expressed by multiple cell types including monocytes, macrophages, dendritic cells, NK cells, platelets, and epithelial cells such as retinal pigment epithelial cells. Ligand binding leads to receptor dimerization, and autophosphorylation of tyrosine residues within the cytoplasmic MerTK (Mer) domain. This activates downstream signaling pathways that control cellular adhesion, aggregation, phagocytosis/efferocytosis, proliferation, survival, and migration. MerTK (Mer) ligands include Gas6 and Protein S that in turn may be bound to phosphatidylserine expressed on the outer plasma membrane of cells undergoing apoptosis. This promotes the phagocytosis of apoptotic cells. Through these activities, MerTK (Mer) plays major roles in the development and regulation of hematopoiesis and immunity. Abnormal cellular expression of MerTK (Mer) has been associated with some autoimmune diseases and various cancers.
Development References (3)
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Dransfield I, Zagórska A, Lew ED, Michail K, Lemke G. Mer receptor tyrosine kinase mediates both tethering and phagocytosis of apoptotic cells.. Cell Death Dis. 2015; 6:e1646. (Biology). View Reference
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Gould WR, Baxi SM, Schroeder R, et al. Gas6 receptors Axl, Sky and Mer enhance platelet activation and regulate thrombotic responses.. J Thromb Haemost. 2005; 3(4):733-41. (Biology). View Reference
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Seitz HM, Camenisch TD, Lemke G, Earp HS, Matsushima GK. Macrophages and dendritic cells use different Axl/Mertk/Tyro3 receptors in clearance of apoptotic cells.. J Immunol. 2007; 178(9):5635-42. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
