PE Rat Anti-Mouse MerTK (Mer)

BD Pharmingen™ PE Rat Anti-Mouse MerTK (Mer)

Name: PE Rat Anti-Mouse MerTK (Mer)
Brand: PE Rat Anti-Mouse MerTK (Mer)
SKU: 570197

クローン BB14-16 (RUO)

Two-color flow cytometric analysis of MerTK (Mer) expression on normal Mouse peritoneal cavity cells (PEC). PEC from C57BL/6 Mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553142]. The cells were then stained with Alexa Fluor™ 647 Rat Anti-Mouse F4/80 antibody (Cat. No. 565853) and with either PE Rat IgG1, κ Isotype Control (Cat. No. 553925; Left Plot) or PE Rat Anti-Mouse MerTK (Mer) antibody (Cat. No. 570196/570197; Right Plot) at 0.25 µg/test. DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The bivariate pseudocolor density plot showing the expression of MerTK (Mer) [or Ig Isotype control staining] versus F4/80 was derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) PEC. Flow cytometry and data analysis were performed using a BD FACSCelesta™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

詳細を表示

Spectrum Viewer

Protocol Library

Scientific Resources

カタログ番号:

単位:

容量:

バルク試薬見積の依頼

製品詳細

Brand: BD Pharmingen™

Alternative Name: Mer; proto-oncogene c-Mer; Mertk; Eyk; Nyk

Reactivity: Mouse (QC Testing)

Isotype: Rat IgG1, κ

Immunogen: Recombinant Mouse MerTK Protein

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

Entrez Gene ID: 17289

RRID: AB_3685583

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.

推奨アッセイ手順

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
An isotype control should be used at the same concentration as the antibody of interest.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
For U.S. patents that may apply, see bd.com/patents.

データシート

Technical Data Sheet (TDS)

関連製品

Stain Buffer (FBS) RUO

サイズ 500 mL カタログ番号 554656

Stain Buffer (BSA) RUO

サイズ 500 mL カタログ番号 554657

Alexa Fluor® 647 Rat Anti-Mouse F4/80 RUO

サイズ 0.1 mg カタログ番号 565853

DAPI Solution RUO

サイズ 1 mg カタログ番号 564907

570197 Rev. 1

抗体の詳細

BB14-16

The BB14-16 monoclonal antibody specifically recognizes the Mer tyrosine kinase (MerTK). MerTK is a single-pass type I transmembrane glycoprotein that is encoded by Mertk (c-mer proto-oncogene tyrosine kinase) which is also known as Mer. MerTK (Mer) is comprised of an extracellular region with two immunoglobulin (Ig)-like domains and two fibronectin type III (FNIII) domains, a transmembrane segment, and a conserved intracellular tyrosine kinase domain. MerTK (Mer) belongs to the TAM subfamily of receptor tyrosine kinases (RTK) which includes Axl and Tyro-3. MerTK (Mer) is variably expressed by multiple cell types including monocytes, macrophages, dendritic cells, NK cells, platelets, and epithelial cells such as retinal pigment epithelial cells. Ligand binding leads to receptor dimerization, and autophosphorylation of tyrosine residues within the cytoplasmic MerTK (Mer) domain. This activates downstream signaling pathways that control cellular adhesion, aggregation, phagocytosis/efferocytosis, proliferation, survival, and migration. MerTK (Mer) ligands include Gas6 and Protein S that in turn may be bound to phosphatidylserine expressed on the outer plasma membrane of cells undergoing apoptosis. This promotes the phagocytosis of apoptotic cells. Through these activities, MerTK (Mer) plays major roles in the development and regulation of hematopoiesis and immunity. Abnormal cellular expression of MerTK (Mer) has been associated with some autoimmune diseases and various cancers.

570197 Rev. 1

フォーマットの詳細

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

フォーマット: PE

励起源: Yellow-Green 561 nm

最大励起波長: 496 nm, 566 nm

最大蛍光波長: 576 nm

570197 Rev.1

引用＆参考文献

Development References (3)

Dransfield I, Zagórska A, Lew ED, Michail K, Lemke G. Mer receptor tyrosine kinase mediates both tethering and phagocytosis of apoptotic cells.. Cell Death Dis. 2015; 6:e1646. (Biology). View Reference
Gould WR, Baxi SM, Schroeder R, et al. Gas6 receptors Axl, Sky and Mer enhance platelet activation and regulate thrombotic responses.. J Thromb Haemost. 2005; 3(4):733-41. (Biology). View Reference
Seitz HM, Camenisch TD, Lemke G, Earp HS, Matsushima GK. Macrophages and dendritic cells use different Axl/Mertk/Tyro3 receptors in clearance of apoptotic cells.. J Immunol. 2007; 178(9):5635-42. (Biology). View Reference

570197 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.