BV421 Mouse Anti-Human Pre-BCR

BD Horizon™ BV421 Mouse Anti-Human Pre-BCR

Name: BV421 Mouse Anti-Human Pre-BCR
Brand: BV421 Mouse Anti-Human Pre-BCR
SKU: 570135

クローン HSL2 (RUO)

Flow cytometric analysis of total Pre-BCR expression by 697 cells. Cells from the Human 697 Pre-B leukemia cell line cells were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Perm/Wash™ Buffer (Cat. No. 554723). The cells were then stained in BD Perm/Wash™ Buffer with either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438; dashed line histogram) or with BD Horizon™ BV421 Mouse Anti-Human Pre-BCR antibody (Cat. No. 570054/570135; solid line histogram) at 0.25 µg/test. The fluorescence histogram showing cell surface and intracellular Pre-BCR expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact 697 cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

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Flow cytometric analysis of Pre-BCR expression on viable Human 697 cells. Cells from the Human 697 Pre-B leukemia cell line were stained with either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438; dashed line histogram) or BD Horizon™ BV421 Mouse Anti-Human Pre-BCR antibody (Cat. No. 570054/570135; solid line histogram) at 0.25 µg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The fluorescence histogram showing the expression of Pre-BCR (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD-negative) 697 cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

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カタログ番号:568022

希望小売価格:¥29,040

単位:EA (1 Each)

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製品詳細

Brand: BD Horizon™

Alternative Name: Pre-B Cell Receptor

Reactivity: Human (QC Testing)

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Human Pre-BCR Complexes Purified from Ltk-Pre-BCR Transfectants

Application: Flow cytometry (Routinely Tested), Intracellular staining (flow cytometry) (Tested During Development)

Concentration: 0.2 mg/ml

Entrez Gene ID: 613

RRID: AB_3685487

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

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BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime BD Horizon Brilliant dyes are used in a multicolor flow cytometry panel. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
For U.S. patents that may apply, see bd.com/patents.

データシート

Technical Data Sheet (TDS)

関連製品

Stain Buffer (FBS) RUO

サイズ 500 ML カタログ番号 554656

Stain Buffer (BSA) RUO

サイズ 500 ML カタログ番号 554657

Brilliant Stain Buffer RUO

サイズ 1000 Tests カタログ番号 566349

Brilliant Stain Buffer Plus RUO

サイズ 1000 Tests カタログ番号 566385

Fixation Buffer RUO

サイズ 100 ML カタログ番号 554655

Perm/Wash Buffer RUO

サイズ 100 ML カタログ番号 554723

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570135 Rev. 1

抗体の詳細

HSL2

The HSL2 monoclonal antibody specifically recognizes the human Pre-B cell Receptor (Pre-BCR) that is comprised of the µ heavy chain (µH chain or µHC) and a surrogate light chain (SL chain or SLC) formed by the noncovalent association of CD179a (VpreB) and CD179b (λ5), also known as either CD179a/CD179b or VpreB/λ5). The HSL2 antibody binds to a unique conformational epitope of the Pre-BCR. The Pre-BCR associates with the signal transducing heterodimer, CD79a (Igα):CD79b (Igβ), to form the Pre-BCR Complex. The Pre-BCR is expressed on Pro-B and early Pre-B cells and plays a critical role in B cell development.

570135 Rev. 1

フォーマットの詳細

BV421

The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

フォーマット: BV421

最大励起波長: 407 nm

最大蛍光波長: 423 nm

570135 Rev.1

引用＆参考文献

View Development References

Development References (2)

Tsuganezawa K, Kiyokawa N, Matsuo Y, et al. Expression profile of pre B-cell receptor components in acute lymphoblastic leukemia and its application to the diagnosis and classification of the disease. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:115-117.
Tsuganezawa K, Kiyokawa N, Matsuo Y, et al. Flow cytometric diagnosis of the cell lineage and developmental stage of acute lymphoblastic leukemia by novel monoclonal antibodies specific to human pre-B-cell receptor.. Blood. 1998; 92(11):4317-24. (Immunogen). View Reference

570135 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.