PE Mouse Anti-Human PSMA

BD Pharmingen™ PE Mouse Anti-Human PSMA

Name: PE Mouse Anti-Human PSMA
Brand: PE Mouse Anti-Human PSMA
SKU: 569997

クローン 107-1A4 (RUO)

Flow cytometric analysis of PSMA expression on Human LNCaP cells. Cells from the Human LNCaP (Prostate Carcinoma, ATCC CRL-1740™) cell line were preincubated with Human BD Fc Block™ (Cat. No. 564219/564220) and stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histogram) or PE Mouse Anti-Human PSMA antibody (Cat. No. 569996/569997; solid line histogram) at 0.5 µg/test. DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histograms showing PSMA expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) LNCaP cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

詳細を表示

Spectrum Viewer

Protocol Library

Scientific Resources

カタログ番号:659453

希望小売価格:¥103,000

単位:EA (1 Each)

容量:

数量:

見積に追加

見積リストに保存バルク試薬見積の依頼

製品詳細

Brand: BD Pharmingen™

Alternative Name: FGCP; FOLH1; GCP2; GCPII; NAALADase I; PSM; mGCP

Reactivity: Human (QC Testing)

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Normal Human Prostate Homogenate and LNCaP Cell Line

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

Entrez Gene ID: 2346

RRID: AB_3685453

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.

推奨アッセイ手順

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
An isotype control should be used at the same concentration as the antibody of interest.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
For U.S. patents that may apply, see bd.com/patents.

データシート

Technical Data Sheet (TDS)

関連製品

Stain Buffer (FBS) RUO

サイズ 500 mL カタログ番号 554656

Stain Buffer (BSA) RUO

サイズ 500 mL カタログ番号 554657

Human BD Fc Block™ RUO

サイズ 0.25 mg カタログ番号 564220

PE Mouse IgG1, κ Isotype Control RUO

サイズ 0.1 mg カタログ番号 554680

DAPI Solution RUO

サイズ 1 mg カタログ番号 564907

569997 Rev. 1

抗体の詳細

107-1A4

The 107-1A4 monoclonal antibody specifically recognizes human Prostate-Specific Membrane Antigen (PSMA) which is also known as Glutamate carboxypeptidase II (GCPII), and N-acetyl-L-aspartyl-L-glutamate peptidase I (NAALADase I). PSMA is an ~100 kDa type II transmembrane glycoprotein. It is comprised of a large extracellular region with protease, apical and C-terminal domains that function in substrate binding and enzymatic activity, a transmembrane region, and a short intracellular N-terminal domain. PSMA is encoded by FOLH1 (Folate hydrolase 1) which belongs to the M28 peptidase family. PSMA is expressed in the brain and by cells in various tissues including the prostate, intestines, testis, bladder, liver, kidney, breast, and ovary. In the intestines, this enzyme functions as a folate hydrolase that cleaves glutamate from dietary folates to facilitate the cellular uptake of folic acid. Through its NAALADase activity, this enzyme also acts in the nervous system to hydrolyze the N-aceylaspartylglutamate (NAAG) neuropeptide into NAA and glutamate. This enzyme can thereby modulate excitatory neurotransmission with the release of glutamate which serves as an excitatory neurotransmitter. Overexpression of PSMA by cancer cells, such as prostate cancer cells, may allow for the increased uptake of folates required for rapid cellular division leading to tumor progression. PSMA expression has also been found by some urothelial adenocarcinoma cells and associated with the tumor neovasculature of some tumors as well. The 107-1A4 antibody reportedly recognizes a distinct conformational epitope in the PSMA extracellular domain.

569997 Rev. 1

フォーマットの詳細

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

フォーマット: PE

励起源: Yellow-Green 561 nm

最大励起波長: 496 nm, 566 nm

最大蛍光波長: 576 nm

569997 Rev.1

引用＆参考文献

Development References (5)

Brown LG, Wegner SK, Wang H, et al. A novel monoclonal antibody 107-1A4 with high prostate specificity: generation, characterization of antigen expression, and targeting of human prostate cancer xenografts.. Prostate Cancer Prostatic Dis. 1998; 1(4):208-215. (Immunogen: ELISA, Immunofluorescence, Immunoprecipitation). View Reference
Chauchereau A, Al Nakouzi N, Gaudin C, et al. Stemness markers characterize IGR-CaP1, a new cell line derived from primary epithelial prostate cancer.. Exp Cell Res. 2011; 317(3):262-75. (Clone-specific: Flow cytometry). View Reference
Mhawech-Fauceglia P, Zhang S, Terracciano L, et al. Prostate-specific membrane antigen (PSMA) protein expression in normal and neoplastic tissues and its sensitivity and specificity in prostate adenocarcinoma: an immunohistochemical study using mutiple tumour tissue microarray technique.. Histopathology. 2007; 50(4):472-83. (Biology: Immunohistochemistry). View Reference
Tykvart J, Navrátil V, Sedlák F, et al. Comparative analysis of monoclonal antibodies against prostate-specific membrane antigen (PSMA).. Prostate. 2014; 74(16):1674-90. (Clone-specific: Flow cytometry, Immunohistochemistry). View Reference
Wang S, Diamond DL, Hass GM, Sokoloff R, Vessella RL. Identification of prostate specific membrane antigen (PSMA) as the target of monoclonal antibody 107-1A4 by proteinchip; array, surface-enhanced laser desorption/ionization (SELDI) technology.. Int J Cancer. 2001; 92(6):871-6. (Clone-specific: Array). View Reference

すべて表示する (5)

569997 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.