R718 Mouse Anti-Mouse CD369 (CLEC7A)

BD Horizon™ R718 Mouse Anti-Mouse CD369 (CLEC7A)

Name: R718 Mouse Anti-Mouse CD369 (CLEC7A)
Brand: R718 Mouse Anti-Mouse CD369 (CLEC7A)
SKU: 569951

クローン 2F3 (RUO)

Multicolor flow cytometric analysis of CD369 (CLEC7A) expression on viable Mouse peritoneal exudate cells (PEC). BALB/c Mouse thioglycolate-elicited PEC cells were harvested and pre-incubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553141/553142]. The cells were then stained with PE Rat Anti-Mouse F4/80 antibody (Cat. No. 565410) and with either BD Horizon™ R718 Mouse IgG1, κ Isotype Control (Cat. No. 567470; Left Plot) or BD Horizon™ R718 Mouse Anti-Mouse CD369 (CLEC7A) antibody (Cat. No. 569951/569956; Right Plot) at 1 µg/test. BD Via-Probe™ Cell Viability 7-AAD Solution was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD369 (CLEC7A) [or Ig Isotype control staining] versus F4/80 was derived from 7-AAD-negative gated events with the forward and side light-scatter characteristics of viable PEC cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

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Multicolor flow cytometric analysis of CD369 (CLEC7A) expression on Mouse peripheral blood leukocytes (PBL). BALB/c Mouse peripheral blood leukocytes (PBL) were stained with PE Rat Anti-Mouse Ly-6G antibody (Cat. No. 551461) and with either BD Horizon™ R718 Mouse IgG1, κ Isotype Control (Cat. No. 567470; Left Plot) or BD Horizon™ R718 Mouse Anti-Mouse CD369 (CLEC7A) antibody (Cat. No. 569951/569956; Right Plot) at 1 µg/test. Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The bivariate pseudocolor density plot showing the correlated expression of CD369 (CLEC7A) [or Ig Isotype control staining] versus Ly-6G was derived from gated events with the forward and side light-scatter characteristics of viable leukocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

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カタログ番号:569951

希望小売価格:¥64,400

単位:EA (1 Each)

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数量:

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製品詳細

Brand: BD Horizon™

Alternative Name: Clec7a; CD369; BGR; Beta-glucan receptor; Bgr; Dectin-1; Dectin1; dectin-1

Reactivity: Mouse (QC Testing)

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Mouse Dectin-1 Recombinant Protein

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

Entrez Gene ID: 56644

RRID: AB_3685429

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

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BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
This product is provided under an Agreement between BIOTIUM and BD Biosciences. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Alexa Fluor™ is a trademark of Life Technologies Corporation.
For U.S. patents that may apply, see bd.com/patents.

データシート

Technical Data Sheet (TDS)

関連製品

Stain Buffer (FBS) RUO

サイズ 500 mL カタログ番号 554656

Stain Buffer (BSA) RUO

サイズ 500 mL カタログ番号 554657

Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO

サイズ 0.5 mg カタログ番号 553142

R718 Mouse IgG1, κ Isotype Control RUO

サイズ 50 µg カタログ番号 567470

Lysing Buffer RUO

サイズ 100 mL カタログ番号 555899

PE Rat Anti-Mouse Ly-6G RUO

サイズ 0.2 mg カタログ番号 551461

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569951 Rev. 1

抗体の詳細

2F3

The 2F3 monoclonal antibody specifically recognizes CD369 which is also known as Dectin-1 (Dendritic cell-associated C-type lectin 1) or BGR (Beta-glucan receptor). CD369 is a ~ 43 kDa type II transmembrane C-type lectin that is encoded by Clec7a (C-type lectin domain family 7, member a). The extracellular portion of CD369 (Clec7a) contains a C-terminal stalk with a carbohydrate recognition domain (CRD) that is followed by a transmembrane segment, and an ITAM-containing cytoplasmic tail. This protein is predominantly expressed on monocytes/macrophages, neutrophils and some dendritic cell populations and at a lower level on a sub-population of T cells. CD369 (Clec7a) binds to beta-glucan polymers and functions as a pattern recognition receptor (PRR) in innate immune response to fungal and bacterial pathogens. CD369 mediated signaling may play a role in leucocyte responses, including phagocytosis or enhanced cytokine production. In mice, two functionally different full-length and stalkless isoforms have been characterized. Both isoforms vary in their ability to recognize zymosan and mediate cellular responses upon zymosan recognition.

569951 Rev. 1

フォーマットの詳細

R718

The BD Horizon™ Red 718 (R718) Dye is part of the BD red family of dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 695-nm and an emission maximum (Em Max) at 718-nm. Driven by BD innovation, R718 is designed to be excited by the red laser (627–640-nm) and detected using an optical filter centered near 720-nm (e.g., a 720/40-nm bandpass filter). R718 is a brighter alternative to Alexa Fluor™ 700. R718 is also a bright small molecule alternative to APC-R700 with lower spread into the APC detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

フォーマット: R718

励起源: Red 627-640 nm

最大励起波長: 695 nm

最大蛍光波長: 718 nm

569951 Rev.1

引用＆参考文献

View Development References

Development References (4)

Brown GD, Taylor PR, Reid DM, et al. Dectin-1 is a major beta-glucan receptor on macrophages.. J Exp Med. 2002; 196(3):407-12. (Biology). View Reference
Herre J, Gordon S, Brown GD. Dectin-1 and its role in the recognition of beta-glucans by macrophages.. Mol Immunol. 2004; 40(12):869-76. (Biology). View Reference
Taylor PR, Brown GD, Reid DM, et al. The beta-glucan receptor, dectin-1, is predominantly expressed on the surface of cells of the monocyte/macrophage and neutrophil lineages.. J Immunol. 2002; 169(7):3876-82. (Biology). View Reference
Underhill DM, Rossnagle E, Lowell CA, Simmons RM. Dectin-1 activates Syk tyrosine kinase in a dynamic subset of macrophages for reactive oxygen production.. Blood. 2005; 106(7):2543-50. (Biology). View Reference

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569951 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.