BV421 Rat Anti-Mouse Granzyme C

BD Horizon™ BV421 Rat Anti-Mouse Granzyme C

Name: BV421 Rat Anti-Mouse Granzyme C
Brand: BV421 Rat Anti-Mouse Granzyme C
SKU: 569861

クローン SFC1D8.rMAb (RUO)

Multicolor flow cytometric analysis of Granzyme C expression in activated Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were activated by culture with Recombinant Mouse IL-15 protein (100 ng/ml) for 4 days (37°C). The cells were harvested, washed, preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553141/553142] and then stained with APC Mouse Anti-Mouse NK-1.1 antibody (Cat. No. 550627) and BD Horizon™ Fixable Viability Stain 780 (Cat. No. 565388). The cells were then fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), washed, permeabilized with and stained in BD Perm/Wash™ Buffer (Cat. No. 554723) with either BD Horizon™ BV421 Rat IgG1, κ Isotype Control (Cat. No. 562868; Left Plot) or BD Horizon™ BV421 Rat Anti-Mouse Granzyme C antibody (Cat. No. 569861/569937; Right Plot) at 0.5 µg/test. The bivariate pseudocolor density plot showing the correlated expression of Granzyme C (or Ig Isotype control staining) versus NK-1.1 was derived from gated events with the forward and side light-scatter characteristics of viable (Fixable Viability Stain 780-negative) splenocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

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カタログ番号:569861

希望小売価格:¥82,500

単位:EA (1 Each)

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製品詳細

Brand: BD Horizon™

Alternative Name: Gzmc; cytotoxic cell protease 2; CCP2; Ctla5; Ctla-5

Reactivity: Mouse (QC Testing)

Isotype: Rat IgG1, κ

Immunogen: Purified Recombinant Mouse Granzyme C Protein

Application: Intracellular staining (flow cytometry) (Routinely Tested)

Concentration: 0.2 mg/ml

Entrez Gene ID: 14940

RRID: AB_3685372

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

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BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime BD Horizon Brilliant dyes are used in a multicolor flow cytometry panel. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
For U.S. patents that may apply, see bd.com/patents.

データシート

Technical Data Sheet (TDS)

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BD Phosflow™ Alexa Fluor® 488 Mouse Anti-CREB (pS133) / ATF-1 (pS63)(J151-21) RUO

カタログ番号 558435

569861 Rev. 1

抗体の詳細

SFC1D8.rMAb

The SFC1D8.rMAb is a recombinant Rat IgG1 κ monoclonal antibody with VH and VL regions derived from SFC1D8 hybridoma cells that secrete Armenian Hamster IgG antibodies specific for Mouse Granzyme C. Granzyme C is also known as Cytotoxic cell protease 2 (CCP2) or Ctla5 (Ctla-5). Granzyme C is a serine protease that is encoded by Gzmc (granzyme C) which belongs to the peptidase S1 family. Granzyme C is found in the cytotoxic granules of cytotoxic T lymphocyte and natural killer (NK) effector cells. Upon recognition of target cells, these effector cells can exocytose Granzyme C which induces apoptosis of target cells leading to their externalization of phosphatidylserine, nuclear condensation, mitochondrial swelling, and the single-stranded DNA nicks. Granzyme C can reportedly support cytotoxic T lymphocyte-mediated killing of target cells in the absence of Granzyme A or B.

569861 Rev. 1

フォーマットの詳細

BV421

The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

フォーマット: BV421

励起源: Violet 405 nm

最大励起波長: 407 nm

最大蛍光波長: 423 nm

569861 Rev.1

引用＆参考文献

Development References (9)

Cai SF, Fehniger TA, Cao X, et al. Differential expression of granzyme B and C in murine cytotoxic lymphocytes.. J Immunol. 2009; 182(10):6287-97. (Immunogen: ELISA, Intracellular Staining/Flow Cytometry). View Reference
Cao X, Cai SF, Fehniger TA, et al. Granzyme B and perforin are important for regulatory T cell-mediated suppression of tumor clearance.. Immunity. 2007; 27(4):635-46. (Biology). View Reference
Garcia-Sanz JA, MacDonald HR, Jenne DE, Tschopp J, Nabholz M. Cell specificity of granzyme gene expression.. J Immunol. 1990; 145(9):3111-8. (Biology). View Reference
Getachew Y, Stout-Delgado H, Miller BC, Thiele DL. Granzyme C supports efficient CTL-mediated killing late in primary alloimmune responses.. J Immunol. 2008; 181(11):7810-7. (Biology). View Reference
Janas ML, Groves P, Kienzle N, Kelso A. IL-2 regulates perforin and granzyme gene expression in CD8+ T cells independently of its effects on survival and proliferation.. J Immunol. 2005; 175(12):8003-10. (Biology). View Reference
Jenne D, Rey C, Masson D, et al. cDNA cloning of granzyme C, a granule-associated serine protease of cytolytic T lymphocytes.. J Immunol. 1988; 140(1):318-23. (Biology). View Reference
Johnson H, Scorrano L, Korsmeyer SJ, Ley TJ. Cell death induced by granzyme C.. Blood. 2003; 101(8):3093-101. (Biology). View Reference
Kelso A, Costelloe EO, Johnson BJ, Groves P, Buttigieg K, Fitzpatrick DR. The genes for perforin, granzymes A-C and IFN-gamma are differentially expressed in single CD8(+) T cells during primary activation.. Int Immunol. 2002; 14(6):605-13. (Biology). View Reference
Revell PA, Grossman WJ, Thomas DA, et al. Granzyme B and the downstream granzymes C and/or F are important for cytotoxic lymphocyte functions.. J Immunol. 2005; 174(4):2124-31. (Biology). View Reference

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569861 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

BD Horizon™ BV421 Rat Anti-Mouse Granzyme C

クローン SFC1D8.rMAb (RUO)

Preparation and Storage

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Product Notices

関連製品

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Development References (9)