APC Mouse Anti-Human IL-13RA2 (CD213a2)

BD Pharmingen™ APC Mouse Anti-Human IL-13RA2 (CD213a2)

Name: APC Mouse Anti-Human IL-13RA2 (CD213a2)
Brand: APC Mouse Anti-Human IL-13RA2 (CD213a2)
SKU: 569498

クローン 47 (also known as Clone 47; mAb47; 47 mAb) (RUO)

Flow cytometric analysis of IL-13RA2 (CD213a2) expression on A-375 cells. Cells from the Human A-375 (Malignant melanoma, ATCC® CRL-1619™) cell line were stained with either APC Mouse IgG1, κ Isotype Control (Cat. No. 554681; dashed line histogram) or APC Mouse Anti-Human IL-13RA2 (CD213a2) antibody (Cat. No. 569498/569499; solid line histogram). DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histogram showing IL-13RA2 (CD213a2) expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) A-375 cells. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa™ Cell Analyzer System and FlowJo™ software.

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カタログ番号:569498

希望小売価格:¥57,750

単位:EA (1 Each)

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製品詳細

Brand: BD Pharmingen™

Alternative Name: CD213A2; IL-13 receptor subunit alpha-2; IL-13R; IL-13Rα2; cancer/testis antigen 19

Reactivity: Human (QC Testing)

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Human rhIL13Ra2hFc

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 5 µl/test

Entrez Gene ID: 3598

RRID: AB_3685108

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.

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BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
For U.S. patents that may apply, see bd.com/patents.

データシート

Technical Data Sheet (TDS)

関連製品

Stain Buffer (FBS) RUO

サイズ 500 mL カタログ番号 554656

Stain Buffer (BSA) RUO

サイズ 500 mL カタログ番号 554657

APC Mouse IgG1 κ Isotype Control RUO

サイズ 0.1 mg カタログ番号 554681

DAPI Solution RUO

サイズ 1 mg カタログ番号 564907

569498 Rev. 1

抗体の詳細

Clone 47 is a monoclonal antibody that specifically recognizes the Human Interleukin-13 Receptor Subunit Alpha-2 (IL-13RA2), also known as IL-13Rα2 or CD213a2. IL-13RA2 (CD213a2) is a ~42 kDa type I transmembrane glycoprotein that is encoded by IL13RA2 which belongs to the type I cytokine receptor family. The extracellular domain is comprised of three extracellular fibronectin type III domains and a WSXWS motif that is followed by a transmembrane segment and a short cytoplasmic tail. Although this receptor primarily exists in a membrane-bound form, a soluble form may be generated upon enzymatic cleavage. IL-13RA2 (CD213a2) binds IL-13 with higher affinity than the moderate affinity IL-13RA1 (CD213a) subunit of the Type II IL-4R complex. This heterodimeric receptor complex is comprised of IL-4RA (CD124) and IL-13RA1 (CD213a) and transduces signals in response to either bound IL-4 or IL-13 ligands. IL-13RA2 (CD213a2) thus appears to act as a decoy receptor due to its short cytoplasmatic tail and lack of signaling motifs and thus controls the bioavailability and activity of IL-13. It is expressed by a subset of peripheral blood lymphocytes and immature dendritic cells. It is also known as cancer/testis antigen 19 (CT19) and may be highly expressed by a variety of malignant cells including high-grade gliomas.

569498 Rev. 1

フォーマットの詳細

APC

Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

フォーマット: APC

励起源: Red 627-640 nm

最大励起波長: 651 nm

最大蛍光波長: 660 nm

569498 Rev.1

引用＆参考文献

Development References (8)

Balyasnikova IV, Wainwright DA, Solomaha E, et al. Characterization and immunotherapeutic implications for a novel antibody targeting interleukin (IL)-13 receptor α2.. J Biol Chem. 2012; 287(36):30215-27. (Immunogen). View Reference
Hershey GK. IL-13 receptors and signaling pathways: an evolving web.. J Allergy Clin Immunol. 2003; 111(4):677-90; quiz 691. (Biology). View Reference
Lupardus PJ, Birnbaum ME, Garcia KC. Molecular basis for shared cytokine recognition revealed in the structure of an unusually high affinity complex between IL-13 and IL-13Ralpha2.. Structure. 2010; 18(3):332-42. (Biology). View Reference
Penke LR, Ouchi H, Speth JM, et al. Transcriptional regulation of the IL-13Rα2 gene in human lung fibroblasts.. Sci Rep. 2020; 10(1):1083. (Biology). View Reference
Tabata Y, Khurana Hershey GK. IL-13 receptor isoforms: breaking through the complexity.. Curr Allergy Asthma Rep. 2007; 7(5):338-45. (Biology). View Reference
Takenouchi M, Hirai S, Sakuragi N, Yagita H, Hamada H, Kato K. Epigenetic modulation enhances the therapeutic effect of anti-IL-13R(alpha)2 antibody in human mesothelioma xenografts.. Clin Cancer Res. 2011; 17(9):2819-29. (Biology). View Reference
Thaci B, Brown CE, Binello E, Werbaneth K, Sampath P, Sengupta S. Significance of interleukin-13 receptor alpha 2-targeted glioblastoma therapy.. Neuro Oncol. 2014; 16(10):1304-12. (Biology). View Reference
Zola H, Swart B, Nicholson I, Voss E. CD213α1 and 2. In: Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007:359-360.

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569498 Rev. 1

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