R718 Mouse Anti-HCV Core Protein

BD Horizon™ R718 Mouse Anti-HCV Core Protein

Name: R718 Mouse Anti-HCV Core Protein
Brand: BD Horizon™
SKU: 569375

クローン C7-50

(RUO)

Flow cytometric analysis of HCV Core Protein expression in HCV Genotype 1b-transfected cells. HCV Core Protein Genotype 1b-transfected cells were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with Perm/Wash Buffer (Cat. No. 554723). The cells were then stained with either BD Horizon™ R718 Mouse IgG1, κ Isotype Control (Cat. No. 567470; dashed line histogram) or BD Horizon™ R718 Mouse Anti-HCV Core Protein antibody (Cat. No. 569374/569375; solid line histogram) at 1 µg/test. The fluorescence histogram showing HCV Core Protein expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis was performed using a BD LSRFortessa™ X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.

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製品詳細

Brand: BD Horizon™

Alternative Name: HCV Core Protein

Reactivity: Viral (QC Testing)

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Purified HCV Core Recombinant Protein

Application: Intracellular staining (flow cytometry) (Routinely Tested)

Concentration: 0.2 mg/ml

Entrez Gene ID: 951475

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed.

推奨アッセイ手順

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
This product is provided under an Agreement between BIOTIUM and BD Biosciences. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
Alexa Fluor™ is a trademark of Life Technologies Corporation.
For U.S. patents that may apply, see bd.com/patents.

関連製品

Stain Buffer (FBS) RUO

サイズ 500 mL カタログ番号 554656

Stain Buffer (BSA) RUO

サイズ 500 mL カタログ番号 554657

R718 Mouse IgG1, κ Isotype Control RUO

サイズ 50 µg カタログ番号 567470

Fixation Buffer RUO

サイズ 100 mL カタログ番号 554655

Perm/Wash Buffer RUO

サイズ 100 mL カタログ番号 554723

抗体の詳細

C7-50

The C7-50 monoclonal antibody specifically recognizes the Hepatitis C Virus (HCV) Genotype 1b Core Protein. This antibody binds to a linear epitope within amino acids 21 to 40 that is highly conserved among all HCV genotypes. HCV is a positive single-stranded RNA virus with a genome of 9,600 nucleotides that encodes a single polyprotein. This polyprotein is post-translationally processed into several structural and non-structural proteins required for viral replication. The HCV core protein is a highly basic RNA-binding protein that surrounds the genome to form the viral nucleocapsid. C7-50 has been used for studying HCV in experimental model systems, including HCV gene-transfected cells.

フォーマットの詳細

R718

The BD Horizon™ Red 718 (R718) Dye is part of the BD red family of dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 695-nm and an emission maximum (Em Max) at 718-nm. Driven by BD innovation, R718 is designed to be excited by the red laser (627–640-nm) and detected using an optical filter centered near 720-nm (e.g., a 720/40-nm bandpass filter). R718 is a brighter alternative to Alexa Fluor™ 700. R718 is also a bright small molecule alternative to APC-R700 with lower spread into the APC detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

フォーマット: R718

励起源: Red 627-640 nm

最大励起波長: 695 nm

最大蛍光波長: 718 nm

引用＆参考文献

Development References (6)

Heintges T, zu Putlitz J, Wands JR. Characterization and binding of intracellular antibody fragments to the hepatitis C virus core protein.. Biochem Biophys Res Commun. 1999; 263(2):410-8. (Clone-specific: Immunoprecipitation, Northern blot, Western blot). View Reference
Kang W, Sung PS, Park SH, et al. Hepatitis C virus attenuates interferon-induced major histocompatibility complex class I expression and decreases CD8+ T cell effector functions.. Gastroenterology. 2014; 146(5):1351-60.e1-4. (Biology). View Reference
Kannan RP, Hensley LL, Evers LE, Lemon SM, McGivern DR. Hepatitis C virus infection causes cell cycle arrest at the level of initiation of mitosis.. J Virol. 2011; 85(16):7989-8001. (Clone-specific: Flow cytometry, Immunofluorescence, Western blot). View Reference
Kato N. Genome of human hepatitis C virus (HCV): gene organization, sequence diversity, and variation.. Microb Comp Genomics. 2000; 5(3):129-51. (Biology). View Reference
Moradpour D, Wakita T, Tokushige K, Carlson RI, Krawczynski K, Wands JR. Characterization of three novel monoclonal antibodies against hepatitis C virus core protein.. J Med Virol. 1996; 48(3):234-41. (Immunogen: ELISA, Immunofluorescence, Radioimmunoassay, Western blot). View Reference
Yasui K, Wakita T, Tsukiyama-Kohara K, et al. The native form and maturation process of hepatitis C virus core protein.. J Virol. 1998; 72(7):6048-55. (Clone-specific: Immunofluorescence). View Reference

すべて表示する (6)

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.