BV421 Mouse Anti-Human CD104 (Integrin β4)

BD Horizon™ BV421 Mouse Anti-Human CD104 (Integrin β4)

Name: BV421 Mouse Anti-Human CD104 (Integrin β4)
Brand: BV421 Mouse Anti-Human CD104 (Integrin β4)
SKU: 569352

クローン ASC-8 (RUO)

Flow cytometric analysis of CD104 (Integrin β4) expression on Human A-431 cells. Cells from the Human A-431 (Human epidermoid carcinoma, ATCC® CRL-1555™) cell line were stained with either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438; dashed line histogram) or BD Horizon™ BV421 Mouse Anti-Human CD104 (Integrin β4) antibody (Cat. No. 569351/569352; solid line histogram). BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The fluorescence histogram showing CD104 (Integrin β4) expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD-negative) cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.

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カタログ番号:569352

希望小売価格:¥41,580

単位:EA (1 Each)

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製品詳細

Brand: BD Horizon™

Alternative Name: ITGB4; CD104; integrin beta-4

Reactivity: Human (QC Testing)

Isotype: Mouse IgG1, κ

Immunogen: SCC9 Human Squamous Carcinoma Cells

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 5 µl/test

Workshop Number: VI A008

Entrez Gene ID: 3691

RRID: AB_3684998

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.

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BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant™ dyes are used in a multicolor flow cytometry panel. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant™ Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant™ Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant™ Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant™ Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant™ Stain Buffer Plus (Cat. No. 566385).

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
For U.S. patents that may apply, see bd.com/patents.

データシート

Technical Data Sheet (TDS)

関連製品

Stain Buffer (FBS) RUO

サイズ 500 ML カタログ番号 554656

Stain Buffer (BSA) RUO

サイズ 500 ML カタログ番号 554657

Brilliant Stain Buffer RUO

サイズ 1000 Tests カタログ番号 566349

Brilliant Stain Buffer Plus RUO

サイズ 1000 Tests カタログ番号 566385

BV421 Mouse IgG1, k Isotype Control RUO

サイズ 50 µg カタログ番号 562438

Cell Viability Solution RUO

サイズ 500 Tests カタログ番号 555815

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569352 Rev. 1

抗体の詳細

ASC-8

The ASC-8 monoclonal antibody specifically recognizes CD104 which is also known as Integrin beta 4 (Integrin β4 or β4 Integrin). Integrins mediate important intercellular and cell-extracellular matrix interactions which are essential for cellular adhesion and migration as well as for regulating cellular growth and survival. CD104 (Integrin β4) is an ~205 kDa single-pass type I transmembrane glycoprotein that is encoded by ITGB4 (Integrin subunit beta 4). CD104 (Integrin β4) noncovalently associates with CD49f (Integrin α6 chain) to form the Integrin α6/β4 (CD49f/CD104) complex. CD104 (Integrin β4) is expressed on basal epithelial cells, Schwann cells, and some endothelial cells and tumor cells. The CD49f/CD104 complex serves as an adhesion receptor that binds to laminins and keratin filaments. It is a component of epidermal hemidesmosomes that facilitate the stable adhesion of basal epithelial cells to the underlying basement membrane.

569352 Rev. 1

フォーマットの詳細

BV421

The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

フォーマット: BV421

最大励起波長: 407 nm

最大蛍光波長: 423 nm

569352 Rev.1

引用＆参考文献

View Development References

Development References (10)

Egles C, Huet HA, Dogan F, et al. Integrin-blocking antibodies delay keratinocyte re-epithelialization in a human three-dimensional wound healing model.. PLoS One. 2010; 5(5):e10528. (Clone-specific: Flow cytometry, Functional assay). View Reference
Hemler ME, Crouse C, Sonnenberg A. Association of the VLA alpha 6 subunit with a novel protein. A possible alternative to the common VLA beta 1 subunit on certain cell lines. J Biol Chem. 1989; 264(11):6529-6535. (Biology). View Reference
Jones JC, Kurpakus MA, Cooper HM, Quaranta V. A function for the integrin alpha 6 beta 4 in the hemidesmosome. Cell Regul. 1991; 2(6):427-438. (Biology). View Reference
Kennel SJ, Epler RG, Lankford TK, et al. Second generation monoclonal antibodies to the human integrin alpha 6 beta 4. Hybridoma. 1990; 9(3):243-255. (Biology). View Reference
Mohri T, Adachi Y, Ikehara S, Hioki K, Tokunaga R, Taketani S. Activated Rac1 selectively up-regulates the expression of integrin alpha6beta4 and induces cell adhesion and membrane ruffles of nonadherent colon cancer Colo201 cells.. Exp Cell Res. 1999; 253(2):533-40. (Clone-specific: Flow cytometry, Functional assay). View Reference
Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
Skubitz AP, Bast RC, Wayner EA, Letourneau PC, Wilke MS. Expression of alpha 6 and beta 4 integrins in serous ovarian carcinoma correlates with expression of the basement membrane protein laminin.. Am J Pathol. 1996; 148(5):1445-61. (Immunogen: Flow cytometry). View Reference
Sonnenberg A, Calafat J, Janssen H, et al. Integrin alpha 6/beta 4 complex is located in hemidesmosomes, suggesting a major role in epidermal cell-basement membrane adhesion.. J Cell Biol. 1991; 113(4):907-17. (Biology). View Reference
Tamura RN, Rozzo C, Starr L, et al. Epithelial integrin alpha 6 beta 4: complete primary structure of alpha 6 and variant forms of beta 4.. J Cell Biol. 1990; 111(4):1593-604. (Biology). View Reference
Tanaka Y, Adachi T, Gianocotti F. CD104 Workshop Panel report. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:432-434.

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569352 Rev. 1

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