Alexa Fluor™ 647 Rabbit Anti-Human PRDX2

BD Pharmingen™ Alexa Fluor™ 647 Rabbit Anti-Human PRDX2

Name: Alexa Fluor™ 647 Rabbit Anti-Human PRDX2
Brand: Alexa Fluor™ 647 Rabbit Anti-Human PRDX2
SKU: 569341

クローン EPR5154 (RUO)

Flow cytometric analysis of PRDX2 expression in HEK-293T cells. Cells from the Human HEK293T (Embryonic Kidney, ATCC® CRL-1573™) cell line were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050). The cells were then stained with either Alexa Fluor™ 647 Rabbit IgG Isotype Control (Cat. No. 569345; dashed line histogram) or Alexa Fluor™ 647 Rabbit Anti-Human PRDX2 antibody (Cat. No. 569341; solid line histogram) at 0.125 µg/test. The fluorescence histogram showing the expression of PRDX2 (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

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カタログ番号:569341

希望小売価格:¥135,300

単位:EA (1 Each)

容量:

50 µg

数量:

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製品詳細

Brand: BD Pharmingen™

Alternative Name: NKEF-B; PRDX2; PRP; PRX2; natural killer cell-enhancing factor B

Reactivity: Human (QC Testing), Mouse,Rat (Reported)

Isotype: Rabbit IgG

Immunogen: Human Peroxiredoxin-2

Application: Intracellular staining (flow cytometry) (Routinely Tested)

Concentration: 0.2 mg/ml

RRID: AB_3684990

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed.

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BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
This rabbit monoclonal (RabMabTM) product is, in part, provided under an intellectual property license from Abcam. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product for buyer’s internal research use only (whether the buyer is an academic or for-profit entity) and buyer shall not be permitted to reverse engineer or otherwise perform any compositional, structural, functional or other analyses directed to learning the methodology, formulae, sequences, process, make-up or production of this product (or any portion thereof). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. If you, as the buyer, do not agree to these conditions, please leave the product unopened and return the contents of this package to BD Biosciences, 10975 Torreyana Road, San Diego, CA 92121. For information on purchasing a license to this product for purposes other than research, contact Abcam at partnerships@abcam.com.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Alexa Fluor™ is a trademark of Life Technologies Corporation.
For U.S. patents that may apply, see bd.com/patents.

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データシート

Technical Data Sheet (TDS)

569341 Rev. 2

抗体の詳細

EPR5154

The EPR5154 recombinant monoclonal antibody specifically recognizes Peroxiredoxin-2 (PRDX2), which is also known as PRX2, PRP, NKEF-B or Natural killer cell-enhancing factor B. PRDX2 is a ~ 22 kDa cytosolic protein that is encoded by PRDX2, which belongs to the peroxiredoxin family within the AhpC/Prx1 subfamily of antioxidant defense enzymes. Peroxiredoxins are highly active and important cysteine-dependent peroxidases with redox sensor activity that regulate peroxide and peroxynitrite levels within most tissues and cells. In humans, six isoforms exist (PRDX1-6) and are widely distributed in the cell, PRDX1 (nucleus and cytoplasm), PRDX2 and PRDX6 (cytoplasm), PRDX3 (mitochondria), PRDX4 (endoplasmic reticulum) and PRDX5 (peroxisomes, cytoplasm and mitochondria). PRDX2 is ubiquitously and variably expressed in cells and is the third most abundant protein and one of the leading cytoprotective agents against oxidative damage from reactive oxygen species (ROS). This intracellular thiol-specific peroxidase acts as a sensor of hydrogen peroxide-mediated events by regulating intracellular hydrogen peroxide concentrations and plays a critical role in the modulation of cell survival. Recent reports have also shown that PRDX2 from macrophages can alter the redox status of cell surface receptors and favor the induction of the inflammatory response by the release of TNF, including the activation of NK cells. This protein may have a proliferative effect and play a role in cancer development as a protumor regulator involved in tumorigenesis and tumor progression.

569341 Rev. 2

フォーマットの詳細

Alexa Fluor™ 647

Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor™ 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

フォーマット: Alexa Fluor™ 647

励起源: Red 627-640 nm

最大励起波長: 653 nm

最大蛍光波長: 669 nm

569341 Rev.2

引用＆参考文献

Development References (12)

Ahl PJ, Hopkins RA, Xiang WW, et al. Met-Flow, a strategy for single-cell metabolic analysis highlights dynamic changes in immune subpopulations.. Communications Biology. 2020; 3(1):1-15. (Clone-specific: Flow cytometry). View Reference
Chen Y, Yang S, Zhou H, Su D. PRDX2 Promotes the Proliferation and Metastasis of Non-Small Cell Lung Cancer In Vitro and In Vivo.. Biomed Res Int. 2020; 2020:8359860. (Biology). View Reference
Gamradt S, Hasselmann H, Taenzer A, et al. Reduced mitochondrial respiration in T cells of patients with major depressive disorder.. iScience. 2021; 24(11):103312. (Clone-specific: Flow cytometry). View Reference
Hussain T, Tan B, Yin Y, Blachier F, Tossou MC, Rahu N. Oxidative Stress and Inflammation: What Polyphenols Can Do for Us?. Oxid Med Cell Longev. 2016; 2016:7432797. (Biology). View Reference
Ishii T, Warabi E, Yanagawa T. Novel roles of peroxiredoxins in inflammation, cancer and innate immunity.. J Clin Biochem Nutr. 2012; 50(2):91-105. (Biology). View Reference
Kang SW, Chae HZ, Seo MS, Kim K, Baines IC, Rhee SG. Mammalian peroxiredoxin isoforms can reduce hydrogen peroxide generated in response to growth factors and tumor necrosis factor-alpha.. J Biol Chem. 1998; 273(11):6297-302. (Biology). View Reference
Knoops B, Argyropoulou V, Becker S, Ferté L, Kuznetsova O. Multiple Roles of Peroxiredoxins in Inflammation.. Mol Cells. 2016; 39(1):60-4. (Biology). View Reference
Krycer JR, Elkington SD, Diaz-Vegas A, et al. Mitochondrial oxidants, but not respiration, are sensitive to glucose in adipocytes.. J Biol Chem. 2020; 295(1):99-110. (Clone-specific: Western blot). View Reference
Lennicke C, Rahn J, Lichtenfels R, Wessjohann LA, Seliger B. Hydrogen peroxide - production, fate and role in redox signaling of tumor cells.. Cell Commun Signal. 2015; 13:39. (Biology). View Reference
Liu J, Su G, Gao J, Tian Y, Liu X, Zhang Z. Effects of Peroxiredoxin 2 in Neurological Disorders: A Review of its Molecular Mechanisms.. Neurochem Res. 2020; 45(4):720-730. (Biology: Flow cytometry). View Reference
Stresing V, Baltziskueta E, Rubio N, et al. Peroxiredoxin 2 specifically regulates the oxidative and metabolic stress response of human metastatic breast cancer cells in lungs.. Oncogene. 2013; 32(6):724-35. (Biology). View Reference
Xirouchaki CE, Jia Y, McGrath MJ, et al. Skeletal muscle NOX4 is required for adaptive responses that prevent insulin resistance.. Sci Adv. 2021; 7(51):eabl4988. (Clone-specific: Western blot). View Reference

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569341 Rev. 2

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.