Preparation and Storage
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BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime BD Horizon Brilliant dyes are used in a multicolor flow cytometry panel. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
関連製品
The HMN4-14 monoclonal antibody specifically recognizes an extracellular domain of mouse Neurogenic locus notch homolog protein 4 (Notch4 or Notch 4). Notch4 is a type 1 transmembrane glycoprotein receptor that is encoded by Notch4 (notch 4) which belongs to the Notch family containing four members, Notch1-Notch4. The Notch4 precursor is cleaved in the Golgi and presents as a cell surface heterodimeric receptor. The Notch4 receptor can bind to several membrane-bound ligands from the Delta-like or Jagged family. Upon ligand binding, Notch4 undergoes proteolytic cleavage that results in the release of the Notch intracellular domain, NICD. NICD translocates to the nucleus where it can form a transcriptional activator complex with various transcription factors. These multimeric complexes either positively or negatively regulate the expression of multiple genes including those that orchestrate many facets of embryonic development and the subsequent functioning of organ systems such as the hematopoietic, immune, nervous, and cardiovascular systems. Notch4 is reportedly expressed on CD8+ splenic dendritic cells, endothelial cells, and macrophages. It is weakly expressed on some CD4+CD8+ thymocytes but is not expressed on mature peripheral T cells.
Development References (9)
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Gordon WR, Arnett KL, Blacklow SC. The molecular logic of Notch signaling--a structural and biochemical perspective.. J Cell Sci. 2008; 121(Pt 19):3109-19. (Biology). View Reference
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Koyanagi A, Sekine C, Yagita H. Expression of Notch receptors and ligands on immature and mature T cells.. Biochem Biophys Res Commun. 2012; 418(4):799-805. (Clone-specific: Flow cytometry). View Reference
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Moriyama Y, Sekine C, Koyanagi A, et al. Delta-like 1 is essential for the maintenance of marginal zone B cells in normal mice but not in autoimmune mice.. Int Immunol. 2008; 20(6):763-73. (Immunogen: Flow cytometry). View Reference
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Murata A, Yoshino M, Hikosaka M, et al. An evolutionary-conserved function of mammalian notch family members as cell adhesion molecules.. PLoS ONE. 2014; 9(9):e108535. (Clone-specific: Flow cytometry). View Reference
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Sauma D, Ramirez A, Alvarez K, Rosemblatt M, Bono MR. Notch signalling regulates cytokine production by CD8+ and CD4+ T cells.. Scand J Immunol. 2012; 75(4):389-400. (Clone-specific: Flow cytometry). View Reference
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Sekine C, Koyanagi A, Koyama N, Hozumi K, Chiba S, Yagita H. Differential regulation of osteoclastogenesis by Notch2/Delta-like 1 and Notch1/Jagged1 axes. Arthritis Res Ther. 2012; 14(2):R45-R58. (Clone-specific: Flow cytometry). View Reference
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Sekine C, Moriyama Y, Koyanagi A, et al. Differential regulation of splenic CD8- dendritic cells and marginal zone B cells by Notch ligands.. Int Immunol. 2009; 21(3):295-301. (Immunogen: Flow cytometry). View Reference
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Sekine C, Nanki T, Yagita H. Macrophage-derived delta-like protein 1 enhances interleukin-6 and matrix metalloproteinase 3 production by fibroblast-like synoviocytes in mice with collagen-induced arthritis.. Arthritis Rheumatol. 2014; 66(10):2751-61. (Clone-specific: Flow cytometry). View Reference
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Uyttendaele H, Marazzi G, Wu G, Yan Q, Sassoon D, Kitajewski J. Notch4/int-3, a mammary proto-oncogene, is an endothelial cell-specific mammalian Notch gene.. Development. 1996; 122(7):2251-9. (Biology). View Reference
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