Alexa Fluor™ 647 Mouse Anti-Human TIGIT

BD Pharmingen™ Alexa Fluor™ 647 Mouse Anti-Human TIGIT

Name: Alexa Fluor™ 647 Mouse Anti-Human TIGIT
Brand: Alexa Fluor™ 647 Mouse Anti-Human TIGIT
SKU: 568945

クローン TgMab-2 (RUO)

Multicolor flow cytometric analysis of TIGIT expression on Human peripheral blood leucocytes. Human whole blood was stained with PE Mouse Anti-Human CD56 (NCAM-1) [Cat. No. 555516/561903; Middle Plots], BD Horizon™ BUV395 Mouse Anti-Human CD4 (Cat. No. 564724), and BD Horizon™ BV421 Mouse Anti-Human CD45RO (Cat. No. 562641/562649; Right Plots) antibodies, and with either Alexa Fluor™ 647 Mouse IgG1, κ Isotype Control (Cat. No. 565571; Top Plots) or Alexa Fluor™ 647 Mouse Anti-Human TIGIT antibody (Cat. No. 568944/568945; Bottom Plots) as indicated. Erythrocytes were lysed with BD FACS Lysing™ Solution (Cat. No. 349202). Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.

Left Plots: The pseudocolor density plot showing the correlated expression of TIGIT (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations.

Middle Plots: The pseudocolor density plot showing the correlated expression of TIGIT (or Ig Isotype control staining) versus CD56 (NCAM-1) was derived from gated events with the light scatter characteristics of intact lymphocytes.

Right Plots: The pseudocolor density plot showing the correlated expression of TIGIT (or Ig Isotype control staining) versus CD45RO was derived from CD4-positive gated events with the light scatter characteristics of intact lymphocytes.

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カタログ番号:744254

希望小売価格:¥78,650

単位:EA (1 Each)

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製品詳細

Brand: BD Pharmingen™

Alternative Name: T-cell immunoreceptor with Ig and ITIM domains; VSIG9; VSTM3; WUCAM

Reactivity: Human (QC Testing)

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Human TIGIT Transfected Cell Line

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 5 µl

Entrez Gene ID: 201633

RRID: AB_3684649

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed.

推奨アッセイ手順

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
An isotype control should be used at the same concentration as the antibody of interest.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Alexa Fluor™ is a trademark of Life Technologies Corporation.
For U.S. patents that may apply, see bd.com/patents.

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データシート

Technical Data Sheet (TDS)

関連製品

Stain Buffer (FBS) RUO

サイズ 500 mL カタログ番号 554656

Stain Buffer (BSA) RUO

サイズ 500 mL カタログ番号 554657

Alexa Fluor® 647 Mouse IgG1 κ Isotype Control RUO

サイズ 50 µg カタログ番号 565571

PE Mouse Anti-Human CD56 (NCAM-1) RUO

サイズ 100 Tests カタログ番号 555516

BUV395 Mouse Anti-Human CD4 RUO

サイズ 100 Tests カタログ番号 564724

BV421 Mouse Anti-Human CD45RO RUO

サイズ 100 Tests カタログ番号 562641

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568945 Rev. 2

抗体の詳細

TgMab-2

The TgMab-2 monoclonal antibody specifically recognizes TIGIT (T cell Immunoreceptor with Ig and ITIM domains) which is also known as Vstm3 (V-set and transmembrane domain-containing 3), Vsig9 (V-set and Ig domain-containing 9) and WUCAM (Washington University Cell Adhesion Molecule). TIGIT is a 30-34 kDa single pass type I transmembrane glycoprotein that belongs to the CD28 family within the Ig superfamily. TIGIT has an extracellular region with a V-type Ig-like domain, transmembrane sequence, and a cytoplasmic domain with an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed on NK cells and subsets of activated and memory T cells, regulatory T cells (Treg), and T follicular helper (Tfh) cells. TIGIT binds to CD112 (PVRL2/Nectin-2) and CD155 (PVR/Necl-5) that are expressed on dendritic cells (DC), endothelial cells, fibroblasts, and some tumor cells and can induce IL-10 release and inhibition of IL-12 production. Ligand-bound TIGIT downregulates TCR-mediated T cell activation and proliferation and can block NK cell-mediated cytotoxicity.

568945 Rev. 2

フォーマットの詳細

Alexa Fluor™ 647

Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor™ 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

フォーマット: Alexa Fluor™ 647

励起源: Red 627-640 nm

最大励起波長: 653 nm

最大蛍光波長: 669 nm

568945 Rev.2

引用＆参考文献

View Development References

Development References (3)

Downs-Canner S, Berkey S, Delgoffe GM, et al. Suppressive IL-17A(+)Foxp3(+) and ex-Th17 IL-17A(neg)Foxp3(+) Treg cells are a source of tumour-associated Treg cells.. Nat Commun. 2017; 8:14649. (Biology). View Reference
Yasuma K, Yasunaga J, Takemoto K, et al. HTLV-1 bZIP Factor Impairs Anti-viral Immunity by Inducing Co-inhibitory Molecule, T Cell Immunoglobulin and ITIM Domain (TIGIT).. PLoS Pathog. 2016; 12(1):e1005372. (Biology). View Reference
Yu X, Harden K, Gonzalez LC, Francesco M, et al. The surface protein TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. Nat Immunol. 2009; 10(1):48-57. (Biology). View Reference

568945 Rev. 2

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.