R718 Rat Anti-Galectin-3

BD Horizon™ R718 Rat Anti-Galectin-3

Name: R718 Rat Anti-Galectin-3
Brand: R718 Rat Anti-Galectin-3
SKU: 568405

クローン M3/38 (RUO)

Galectin-3 Expression by Human and Mouse Leucocytes

1. Surface_Neutrophils Human blood was stained with BD Horizon™ R718 Rat IgG2a, κ Isotype Control (Cat. No. 566941; dashed histogram) or BD Horizon™ R718 Rat Anti-Galectin-3 (Cat. No. 568404/568405; solid line histogram) at 1 µg/test. Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). A histogram showing Surface Galectin-3 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable neutrophils.

2. Surface + Intracellular_PBMC Peripheral blood mononuclear cells (PBMC) were preincubated with Human BD Fc Block™ (Cat. No. 564219/564220), fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), and permeabilized with BD Perm/Wash™ Buffer (Cat. No. 554723). Cells were then stained with PE Mouse Anti-Human CD11b (Cat. No. 555388) and with either BD Horizon™ R718 Rat IgG2a, κ Isotype Control or BD Horizon™ R718 Rat Anti-Galectin-3. The bivariate pseudocolor density plot shows the correlated expression of total cellular Galectin-3 (or Ig Isotype control staining) versus CD11b by gated intact PBMC.

3. Surface_PEC Thioglycolate-elicited C57BL/6 Mouse peritoneal exudate cells (Thio-PEC) were preincubated with Purified Rat Anti-Mouse CD16/CD32 Ab (Mouse BD Fc Block™) and stained with either BD Horizon™ R718 Rat IgG2a, κ Isotype Control (dashed line histogram) or BD Horizon™ R718 Rat Anti-Galectin-3 (solid line histogram) at 1 µg/test. The histogram shows Surface Galectin-3 expression (or Ig Isotype control staining) on viable (DAPI-negative) [Cat. 564907] Thio-PEC.

4. Surface + Intracellular_PEC Thio-PEC were similarly fixed, permeabilized and stained (without DAPI). The histogram shows total cellular Galectin-3 expression (or Ig Isotype control staining) by intact Thio-PEC.

Flow cytometry and analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown are not lot specific.

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Galectin-3 Expression by Human and Mouse Leucocytes

Flow cytometry and analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown are not lot specific.

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カタログ番号:568405

希望小売価格:¥25,410

単位:EA (1 Each)

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製品詳細

Brand: BD Horizon™

Alternative Name: CBP 35; LGALS3; Lgals3; MAC2; Mac-2; gal-3; gal3

Reactivity: Human (QC Testing), Mouse (Tested in Development)

Isotype: Rat IgG2a, κ

Immunogen: Mouse Galectin-3

Application: Intracellular staining (flow cytometry) (Routinely Tested)

Concentration: 0.2 mg/ml

RRID: AB_3684246

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed.

推奨アッセイ手順

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
This product is provided under an Agreement between BIOTIUM and BD Biosciences. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
Alexa Fluor™ is a trademark of Life Technologies Corporation.
For U.S. patents that may apply, see bd.com/patents.

データシート

Technical Data Sheet (TDS)

関連製品

Stain Buffer (FBS) RUO

サイズ 500 mL カタログ番号 554656

Stain Buffer (BSA) RUO

サイズ 500 mL カタログ番号 554657

R718 Rat IgG2a, κ Isotype Control RUO

サイズ 50 µg カタログ番号 566941

Human BD Fc Block™ RUO

サイズ 0.25 mg カタログ番号 564220

Fixation Buffer RUO

サイズ 100 mL カタログ番号 554655

Perm/Wash Buffer RUO

サイズ 100 mL カタログ番号 554723

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568405 Rev. 1

抗体の詳細

M3/38

The M3/38 monoclonal antibody specifically recognizes Galectin-3 (Gal-3 or gal3) which is also known as Galactose-specific lectin 3, Mac-2, MAC2, and Carbohydrate-binding protein 35 (CBP 35). Galectin-3 is an ~30-35 kDa protein that includes an N-terminal proline-rich tandem repeat domain as well as a C-terminal region with one carbohydrate recognition domain (CRDs). Galectin-3 is encoded by Lgals3 (lectin, galactose binding, soluble 3) which belongs to the galectin family. It is expressed by a variety of cells including activated macrophages, dendritic cells (DCs), granulocytes, mast cells, and epithelial cells. Galectin-3 can be detected in monomeric or multimeric forms in cytoplasmic, nuclear, cell surface and extracellular locations. Galectin-3 is a β-galactoside-binding lectin that has multiple intracellular and extracellular functions implicated in biological processes including RNA splicing as well as cellular chemoattraction and adhesion, activation, growth, proliferation, differentiation, and apoptosis. Upregulated Galectin-3 expression is implicated in inflammation and disease states including cancer progression and metastasis.

568405 Rev. 1

フォーマットの詳細

R718

The BD Horizon™ Red 718 (R718) Dye is part of the BD red family of dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 695-nm and an emission maximum (Em Max) at 718-nm. Driven by BD innovation, R718 is designed to be excited by the red laser (627–640-nm) and detected using an optical filter centered near 720-nm (e.g., a 720/40-nm bandpass filter). R718 is a brighter alternative to Alexa Fluor™ 700. R718 is also a bright small molecule alternative to APC-R700 with lower spread into the APC detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

フォーマット: R718

励起源: Red 627-640 nm

最大励起波長: 695 nm

最大蛍光波長: 718 nm

568405 Rev.1

引用＆参考文献

View Development References

Development References (8)

Demotte N, Wieërs G, Van Der Smissen P, et al. A galectin-3 ligand corrects the impaired function of human CD4 and CD8 tumor-infiltrating lymphocytes and favors tumor rejection in mice.. Cancer Res. 2010; 70(19):7476-88. (Clone-specific: Flow cytometry). View Reference
Dong R, Zhang M, Hu Q, et al. Galectin-3 as a novel biomarker for disease diagnosis and a target for therapy (Review).. Int J Mol Med. 2018; 41(2):599-614. (Biology). View Reference
Flotte TJ, Springer TA, Thorbecke GJ. Dendritic cell and macrophage staining by monoclonal antibodies in tissue sections and epidermal sheets. Am J Pathol. 1983; 111(1):112-124. (Clone-specific: Immunohistochemistry). View Reference
Gray RM, Davis MJ, Ruby KM, Voss PG, Patterson RJ, Wang JL. Distinct effects on splicing of two monoclonal antibodies directed against the amino-terminal domain of galectin-3.. Arch Biochem Biophys. 2008; 475(2):100-8. (Clone-specific: Functional assay, Western blot). View Reference
Ho MK, Springer TA. Mac-2, a novel 32,000 Mr mouse macrophage subpopulation-specific antigen defined by monoclonal antibodies.. J Immunol. 1982; 128(3):1221-8. (Immunogen: Flow cytometry, Immunoprecipitation, Radioimmunoassay). View Reference
Johannes L, Jacob R, Leffler H. Galectins at a glance.. J Cell Sci. 2018; 131(9):jcs208884. (Biology). View Reference
Karlsson A, Follin P, Leffler H, Dahlgren C. Galectin-3 activates the NADPH-oxidase in exudated but not peripheral blood neutrophils.. Blood. 1998; 91(9):3430-8. (Clone-specific: Flow cytometry). View Reference
Newlaczyl AU, Yu LG. Galectin-3--a jack-of-all-trades in cancer.. Cancer Lett. 2011; 313(2):123-8. (Biology). View Reference

すべて表示する (8)

568405 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.