PE-CF594 Rat Anti-Mouse CD205 (DEC-205)

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BD Horizon™ PE-CF594 Rat Anti-Mouse CD205 (DEC-205)

Name: PE-CF594 Rat Anti-Mouse CD205 (DEC-205)
Brand: PE-CF594 Rat Anti-Mouse CD205 (DEC-205)
SKU: 567652

クローン NLDC-145 (RUO)

Two-color flow cytometric analysis of CD205 (DEC-205) expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with BD Horizon™ BV421 Rat Anti-Mouse Ly-6G and Ly-6C antibody (Cat. No. 562709) and with either BD Horizon™ PE-CF594 Rat IgG2a, κ Isotype Control (Cat. No. 562302; Left Plot) or BD Horizon™ PE-CF594 Rat Anti-Mouse CD205 (DEC-205) antibody (Cat. No. 567652; Right Plot) at 0.25 μg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD205 (DEC-205) [or Ig Isotype control staining] versus Ly-6G and Ly-6C was derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD-negative) cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

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カタログ番号:742692

希望小売価格:¥78,650

単位:EA (1 Each)

容量:

50 µg

数量:

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見積リストに保存バルク試薬見積の依頼

製品詳細

Brand: BD Horizon™

Alternative Name: CD205; Cd205; DEC-205; DEC205; Ly-75; Ly75

Reactivity: Mouse (QC Testing)

Isotype: Rat WI, also known as Wistar (outbred) IgG2a, κ

Immunogen: Mouse lymph node stromal cells

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

Vol. Per Test: NA

RRID: AB_2916684

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.

推奨アッセイ手順

BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and CompBead to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Texas Red is a registered trademark of Molecular Probes, Inc., Eugene, OR.
CF™ is a trademark of Biotium, Inc.
When excited by the yellow-green (561-nm) laser, the fluorescence may be brighter than when excited by the blue (488-nm) laser.
This product is provided under an Agreement between BIOTIUM and BD Biosciences. The manufacture, use, sale, offer for sale, or import of this product is subject to one or more patents or pending applications owned or licensed by Biotium, Inc. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using multi-laser cytometers, which may directly excite both PE and CF™594.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.

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データシート

Technical Data Sheet (TDS)

関連製品

Stain Buffer (FBS) RUO

サイズ 500 mL カタログ番号 554656

Stain Buffer (BSA) RUO

サイズ 500 mL カタログ番号 554657

Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO

サイズ 0.5 mg カタログ番号 553142

BV421 Rat Anti-Mouse Ly-6G and Ly-6C RUO

サイズ 50 µg カタログ番号 562709

PE-CF594 Rat IgG2a, κ Isotype Control RUO

サイズ 0.1 mg カタログ番号 562302

Cell Viability Solution RUO

サイズ 500 Tests カタログ番号 555815

詳細を表示

567652 Rev. 1

抗体の詳細

NLDC-145

567652 Rev. 1

フォーマットの詳細

PE-CF594

BD Horizon™ PE-CF594 dye is a part of the BD PE family of dyes. This tandem fluorochrome is comprised of a R-Phycoerythrin (PE) donor that has excitation maxima (Ex Max) of 496-nm and 566-nm and an acceptor dye with an emission maximum (Em Max) at 615-nm. PE-CF594, driven by BD innovation, is designed to be excited by the blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and detected using an optical filter centered near 615 nm (e.g., a 610/20-nm bandpass filter). The donor dye can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and the acceptor dye can be excited by the green (532-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

フォーマット: PE-CF594

励起源: Yellow-Green 561 nm

最大励起波長: 496 nm, 566 nm

最大蛍光波長: 615 nm

567652 Rev.1

引用＆参考文献

Development References (12)

East L, Isacke CM. The mannose receptor family.. Biochim Biophys Acta. 2002; 1572(2-3):364-86. (Clone-specific). View Reference
Hossain MK, Wall KA. Use of Dendritic Cell Receptors as Targets for Enhancing Anti-Cancer Immune Responses.. Cancers (Basel). 2019; 11(3):E418. (Biology). View Reference
Inaba K, Swiggard WJ, Inaba M, et al. Tissue distribution of the DEC-205 protein that is detected by the monoclonal antibody NLDC-145. I. Expression on dendritic cells and other subsets of mouse leukocytes.. Cell Immunol. 1995; 163(1):148-56. (Clone-specific: Flow cytometry). View Reference
Jiang W, Swiggard WJ, Heufler C. The receptor DEC-205 expressed by dendritic cells and thymic epithelial cells is involved in antigen processing. Nature. 1995; 375(6527):151-155. (Clone-specific). View Reference
Kraal G, Breel M, Janse M, Bruin G. Langerhans' cells, veiled cells, and interdigitating cells in the mouse recognized by a monoclonal antibody. J Exp Med. 1986; 163(4):981-997. (Immunogen). View Reference
Merad M, Sathe P, Helft J, Miller J, Mortha A. The dendritic cell lineage: ontogeny and function of dendritic cells and their subsets in the steady state and the inflamed setting.. Annu Rev Immunol. 2013; 31:563-604. (Biology). View Reference
Steinman RM. Decisions about dendritic cells: past, present, and future.. Annu Rev Immunol. 2012; 30:1-22. (Clone-specific). View Reference
Steinman RM. Dendritic cells: understanding immunogenicity.. Eur J Immunol. 2007; 37 Suppl 1:S53-60. (Clone-specific). View Reference
Swiggard WJ, Mirza A, Nussenzweig MC, Steinman RM. DEC-205, a 205-kDa protein abundant on mouse dendritic cells and thymic epithelium that is detected by the monoclonal antibody NLDC-145: purification, characterization, and N-terminal amino acid sequence.. Cell Immunol. 1995; 165(2):302-11. (Clone-specific: Immunoaffinity chromatography, Immunoprecipitation, Western blot). View Reference
Vremec D, Zorbas M, Scollay R, et al. The surface phenotype of dendritic cells purified from mouse thymus and spleen: investigation of the CD8 expression by a subpopulation of dendritic cells. J Exp Med. 1992; 176(1):47-58. (Clone-specific: Flow cytometry, Fluorescence microscopy, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation). View Reference
Witmer-Pack MD, Swiggard WJ, Mirza A, Inaba K, Steinman RM. Tissue distribution of the DEC-205 protein that is detected by the monoclonal antibody NLDC-145. II. Expression in situ in lymphoid and nonlymphoid tissues.. Cell Immunol. 1995; 163(1):157-62. (Clone-specific: Immunohistochemistry, Western blot). View Reference
Wu L, Vremec D, Ardavin C, et al. Mouse thymus dendritic cells: kinetics of development and changes in surface markers during maturation. Eur J Immunol. 1995; 25(2):418-425. (Clone-specific: Flow cytometry). View Reference

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567652 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.