Multiparameter flow cytometric analysis of SLAMF6 (CD352) expression on human peripheral blood leucocyte populations. Whole blood cells were stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Plot) or PE Mouse Anti-Human SLAMF6 (CD352) antibody (Cat. No. 566378; Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-parameter flow cytometric contour plots showing the correlated expression of SLAMF6 (CD352) [or Ig Isotype control staining] versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD™ Canto II Flow Cytometer System.
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Multiparameter flow cytometric analysis of SLAMF6 (CD352) expression on human peripheral blood leucocyte populations. Whole blood cells were stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Plot) or PE Mouse Anti-Human SLAMF6 (CD352) antibody (Cat. No. 566378; Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-parameter flow cytometric contour plots showing the correlated expression of SLAMF6 (CD352) [or Ig Isotype control staining] versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometric analysis was performed using a BD™ Canto II Flow Cytometer System.
製品詳細
BD Pharmingen™
SLAMF6; NTBA; NTB-A; NKTB-antigen; SLAM family memb. 6; KALI; SF2000; Ly108
Human (QC Testing)
Mouse IgG1, κ
Human SLAMF6 Transfected Cell Line
Flow cytometry (Routinely Tested)
5 µl/test
AB_2739718
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
関連製品
Stain Buffer (FBS) RUO
サイズ
500 mL
カタログ番号
554656
Stain Buffer (BSA) RUO
サイズ
500 mL
カタログ番号
554657
Lysing Buffer RUO
サイズ
100 mL
カタログ番号
555899
Lysing Solution 10X Concentrate RUO (GMP)
サイズ
100 mL
カタログ番号
349202
PE Mouse IgG1, κ Isotype Control RUO
サイズ
0.1 mg
カタログ番号
554680
566378 Rev. 1
抗体の詳細
hSF6.4.20
The hSF6.4.20 monoclonal antibody specifically binds to SLAM family member 6 (SLAMF6) which is also known as CD352, NK-T-B-antigen (NTB-A), or Activating NK receptor. CD352 is a ~60 kDa type I transmembrane glycoprotein that contains two ITSMs (immunoreceptor tyrosine-based switch motifs) in its cytoplasmic domain and belongs to the CD2 subfamily of the immunoglobulin superfamily. CD352 is expressed by thymocytes, NK cells, B cells, and T cells. CD352 functions through homophilic interactions as a coreceptor for the activation of NK cells and lymphocytes. It plays roles in regulating various cellular effector functions including cytolytic activity, production of cytokines such as IFN-γ and IL-17, and immunoglobulin class switching.
566378 Rev. 1
フォーマットの詳細
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
566378 Rev.1
引用＆参考文献
Development References (6)
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Chatterjee M, Hedrich CM, Rauen T, Ioannidis C, Terhorst C, Tsokos GC. CD3-T cell receptor co-stimulation through SLAMF3 and SLAMF6 receptors enhances RORγt recruitment to the IL17A promoter in human T lymphocytes.. J Biol Chem. 2012; 287(45):38168-77. (Biology). View Reference
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Eissmann P, Watzl C.. Molecular analysis of NTB-A signaling: a role for EAT-2 in NTB-A-mediated activation of human NK cells.. J Immunol. 2006; 177(5):3170-3177. (Biology). View Reference
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Flaig RM, Stark S, Watzl C.. Cutting edge: NTB-A activates NK cells via homophilic interaction. J Immunol. 2004; 172(11):6424-6427. (Biology). View Reference
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Kumar KR, Li L, Yan M, et al. Regulation of B cell tolerance by the lupus susceptibility gene Ly108. Science. 2006; 312(5780):1606-1608. (Biology). View Reference
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Ramirez PW, DePaula-Silva AB, Szaniawski M, Barker E, Bosque A, Planelles V. HIV-1 Vpu utilizes both cullin-RING ligase (CRL) dependent and independent mechanisms to downmodulate host proteins.. Retrovirology. 2015; 12:65. (Biology). View Reference
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Valdez PA, Wang H, Seshasayee D, et al. NTB-A, a new activating receptor in T cells that regulates autoimmune disease.. J Biol Chem. 2004; 279(18):18662-18669. (Biology). View Reference
566378 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
