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Purified Mouse Anti-MLC2v
565039
¥37,510
EA (1 Each)
0.1 mg
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製品詳細
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BD Pharmingen™
MLC-2; MLC-2v; MYL2; MLC2; CMH10
Human (QC Testing), Mouse (Predicted)
Mouse IgG1, κ
Human MLC2v
Intracellular staining (flow cytometry) (Routinely Tested), Bioimaging, Immunofluorescence, Immunohistochemistry-formalin (antigen retrieval required), Western blot (Tested During Development)
28 kDa
0.5 mg/ml
AB_2739266
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. An isotype control should be used at the same concentration as the antibody of interest.

データシート

565497 Rev. 1
抗体の詳細
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S53-5

Myosin is composed of 2 heavy chains, 2 regulatory light chains, and 2 alkali light chains. The S53-5 monoclonal antibody specifically binds to human Myosin regulatory light chain 2,ventrical isoform (MLC2v) that is selectively expressed in the cardiac ventrical, while the MLC2a isoform is selectively expressed in the cardiac atrium. The differential expression of the two MLC2 isoforms is first detected early in cardiogenesis, prior to the formation of distinct atrial and ventrical chambers. During the induction of cardiovascular lineage cells from a human embryonic stem cell line, the appearance of contracting cardiomyocytes is coincident with the up-regulation of the expression of several genes, including the genes encoding MLC2a and MLC2v. Cross-reactivity of the S53-5 monoclonal antibody to mouse MLC2v is predicted due to the ~96% conservation of the human and mouse protein sequences.

565497 Rev. 1
フォーマットの詳細
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
565497 Rev.1
引用&参考文献
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Development References (5)

  1. Dubois NC, Craft AM, Sharma P, et al. SIRPA is a specific cell-surface marker for isolating cardiomyocytes derived from human pluripotent stem cells. Nat Biotechnol. 2011; 29:1011-1018. (Biology). View Reference
  2. Franco D, Lamers WH, Moorman AF. Patterns of expression in the developing myocardium: towards a morphologically integrated transcriptional model. Cardiovasc Res. 1998; 38(1):25-53. (Biology). View Reference
  3. Laflamme MA, Murry CE. Heart regeneration. Nature. 2011; 473(7347):326-335. (Biology). View Reference
  4. Lee MY, Sun B, Schliffke S, et al. Derivation of functional ventricular cardiomyocytes using endogenous promoter sequence from murine embryonic stem cells. Stem Cell Res. 2012; 8(1):49-57. (Biology). View Reference
  5. O'Brien TX, Lee KJ, Chien KR. Positional specification of ventricular myosin light chain 2 expression in the primitive murine heart tube. Proc Natl Acad Sci U S A. 1993; 90(11):5157-5161. (Biology). View Reference
すべて表示する (5) 表示項目を減らす
565497 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.