Purified Mouse Anti-MLC2a

BD Pharmingen™ Purified Mouse Anti-MLC2a

Name: Purified Mouse Anti-MLC2a
Brand: Purified Mouse Anti-MLC2a
SKU: 565496

クローン S58-205 (RUO)

Flow cytometric analysis of MLC2a and MLC2v expression in MLC2a-transfected 293F cells. Untransfected (dashed line histogram) and human MLC2a-transfected (solid line histogram) 293F cells were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050). The cells were then washed and stained with Purified Mouse Anti-Human MLC2a (Cat. No. 565496, left panel) and Purified Mouse Anti-Human MLC2v (Cat. No. 565497, middle panel) followed by PE Goat Anti-Mouse Ig (Cat. No. 550589). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.

Western blot analysis of MLC2a expression (right panel). Lysate from MLC2a-transfected 293F cells was prepared for electrophoresis (SDS-PAGE) in a 2D Tris-Glycine polyacrylamide gel. The proteins were transferred to PVDF membranes and then probed with 0.063 (lane 1), 0.032 (lane 2), and 0.016 (lane 3) µg/mL of Purified Mouse Anti-MLC2a. Specific staining was detected with HRP Goat Anti-Mouse Ig (Cat No. 554002).

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Immunohistochemical staining of MLC2a in human left atrium and left ventricle Following antigen retrieval with BD Retrievagen A buffer (Cat. No. 550524), the formalin-fixed paraffin-embedded sections were stained with either Purified Mouse IgG1, κ Isotype Control (Cat. No. 550878; top left) or Purified Mouse Anti-MLC2a (top right) on left atrium. Additionally, Purified Mouse Anti-MLC2a (bottom left) and Purified Mouse Anti-MLC2v (Cat. No. 565497, bottom right) were used to stain left ventricle. A three-step staining procedure that employs Biotin Goat Anti-Mouse Ig (Cat. No. 550337), Streptavidin HRP (Cat. No.550946), and the DAB Substrate Kit (Cat. No. 550880) was used to develop the primary staining reagents. The tissues were counterstained with Hematoxylin. Original magnification: 40X (MLC2a) and 20X (MLC2v).

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カタログ番号:

単位:

容量:

0.1 mg

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製品詳細

Brand: BD Pharmingen™

Alternative Name: MLC-2a; myosin regulatory light chain 7; MYL7; MYL2A; MYLC2A

Reactivity: Human (QC Testing), Mouse (Predicted)

Isotype: Mouse IgG1, κ

Immunogen: Human MLC2a Recombinant Protein

Application: Intracellular staining (flow cytometry) (Routinely Tested), Bioimaging, Immunofluorescence, Immunohistochemistry-formalin (antigen retrieval required), Western blot (Tested During Development)

Target Molecular Weight: 28 kDa

Concentration: 0.5 mg/ml

RRID: AB_2739265

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
An isotype control should be used at the same concentration as the antibody of interest.
Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

データシート

Technical Data Sheet (TDS)

関連製品

Stain Buffer (BSA) RUO

サイズ 500 mL カタログ番号 554657

Stain Buffer (FBS) RUO

サイズ 500 mL カタログ番号 554656

Fixation Buffer RUO

サイズ 100 mL カタログ番号 554655

Perm Buffer III RUO

サイズ 125 mL カタログ番号 558050

Retrievagen A (pH 6.0) RUO

カタログ番号 550524

Purified Mouse IgG1 κ Isotype Control RUO

サイズ 1 mL カタログ番号 550878

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565496 Rev. 1

抗体の詳細

S58-205

Myosin is composed of 2 heavy chains, 2 regulatory light chains, and 2 alkali light chains. The S58-205 monoclonal antibody specifically binds to human Myosin regulatory light chain 2, atrial isoform (MLC2a) that is selectively expressed in the cardiac atrium, while the MLC2v isoform is selectively expressed in the cardiac ventrical. The differential expression of the two MLC2 isoforms is first detected early in cardiogenesis, prior to the formation of distinct atrial and ventrical chambers. During the induction of cardiovascular lineage cells from a human embryonic stem cell line, the appearance of contracting cardiomyocytes is coincident with the up-regulation of the expression of several genes, including the genes encoding MLC2a and MLC2v. Cross-reactivity of the S58-205 monoclonal antibody to mouse MLC2a is predicted due to the ~96% conservation of the human and mouse protein sequences.

565496 Rev. 1

フォーマットの詳細

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

フォーマット: Purified

565496 Rev.1

引用＆参考文献

View Development References

Development References (5)

Dubois NC, Craft AM, Sharma P, et al. SIRPA is a specific cell-surface marker for isolating cardiomyocytes derived from human pluripotent stem cells. Nat Biotechnol. 2011; 29:1011-1018. (Biology). View Reference
Franco D, Lamers WH, Moorman AF. Patterns of expression in the developing myocardium: towards a morphologically integrated transcriptional model. Cardiovasc Res. 1998; 38(1):25-53. (Biology). View Reference
Hailstones D, Barton P, Chan-Thomas P, et al. Differential regulation of the atrial isoforms of the myosin light chains during striated muscle development. J Biol Chem. 1992; 267(32):23295-23300. (Biology). View Reference
Kubalak SW, Miller-Hance WC, O'Brien TX, Dyson E, Chien KR. Chamber specification of atrial myosin light chain-2 expression precedes septation during murine cardiogenesis. J Biol Chem. 1994; 269(24):16961-16970. (Biology). View Reference
Laflamme MA, Murry CE. Heart regeneration. Nature. 2011; 473(7347):326-335. (Biology). View Reference

すべて表示する (5)

565496 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.