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PE Mouse Anti-Human Siglec-1 (CD169)
PE Mouse Anti-Human Siglec-1 (CD169)
Flow cytometric analysis of Siglec-1 (CD169) expression on untreated or Interferon-α (IFN-α)-treated human peripheral blood mononuclear cells. Human PBMC (2 x 10^6 cells/mL in complete tissue culture medium) isolated from a heparinized peripheral whole blood sample were either cultured without (top panels) or with (bottom panels) recombinant human IFN-α2a protein (20 ng/mL) for 48 hours. The cells were washed, preincubated with BD Pharmingen™ Human BD Fc Block™ (Cat. No. 564219/564220), and stained with FITC Mouse Anti-Human CD14 antibody (Cat. No. 555397/561712) and either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Panels) or PE Mouse Anti-Human Siglec-1 (CD169) antibody (Cat. No. 565248; Right Panels). The overlaid contour plots were derived from gated events with forward and side light scatter characteristics of viable CD14-  lymphocytes or CD14+ monocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Flow cytometric analysis of Siglec-1 (CD169) expression on untreated or Interferon-α (IFN-α)-treated human peripheral blood mononuclear cells. Human PBMC (2 x 10^6 cells/mL in complete tissue culture medium) isolated from a heparinized peripheral whole blood sample were either cultured without (top panels) or with (bottom panels) recombinant human IFN-α2a protein (20 ng/mL) for 48 hours. The cells were washed, preincubated with BD Pharmingen™ Human BD Fc Block™ (Cat. No. 564219/564220), and stained with FITC Mouse Anti-Human CD14 antibody (Cat. No. 555397/561712) and either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Panels) or PE Mouse Anti-Human Siglec-1 (CD169) antibody (Cat. No. 565248; Right Panels). The overlaid contour plots were derived from gated events with forward and side light scatter characteristics of viable CD14-  lymphocytes or CD14+ monocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
製品詳細
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BD Pharmingen™
Sialoadhesin; SN; SIGLEC1; Siglec-1; Sialic acid-binding Ig-like lectin 1
Human (QC Testing)
Mouse BALB/c IgG1, κ
Human Rhinovirus-infected Dendritic Cells
Flow cytometry (Routinely Tested)
5 µl/test
6614
AB_2732051
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
565248 Rev. 2
抗体の詳細
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7-239

The 7-239 monoclonal antibody specifically binds to Sialic acid-binding Ig-like lectin 1 (Siglec-1), which is also known as Sialoadhesin (SN), or CD169.  Siglec-1 is a type I transmembrane glycoprotein that belongs to the Siglec family within the Ig superfamily. This adhesion molecule especially binds to glycolipids and glycoproteins with terminal α-2 sialyl residues. Siglec-1 is expressed by macrophages and dendritic cells and serves as a cellular interaction molecule. Its expression can be upregulated by cells in response to type II collagen, or to cytokines including interferons, and tumor necrosis factor. Siglec-1 plays roles in endocytosis, hematopoiesis, and leucocyte migration. It mediates macrophage binding to various cell types including developing and mature leucocytes. Siglec-1 that is expressed by dendritic cells can also bind HIV-1 and may mediate viral transfer to bystander CD4+ T cells. Several Siglec-1 counter-receptors have been described including CD43, CD206, and CD227 which are expressed by T cells, macrophages, or breast cancer cells, respectively. The 7-239 antibody reportedly blocks Siglec-1 functions in some cellular assay systems.

565248 Rev. 2
フォーマットの詳細
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
565248 Rev.2
引用&参考文献
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Development References (6)

  1. Hartnell A, Steel J, Turley H, Jones M, Jackson DG, Crocker PR. Characterization of human sialoadhesin, a sialic acid binding receptor expressed by resident and inflammatory macrophage populations. Blood. 2001; 97(1):288-296. (Biology). View Reference
  2. Izquierdo-Useros N, Lorizate M, Puertas MC, et al. Siglec-1 is a novel dendritic cell receptor that mediates HIV-1 trans-infection through recognition of viral membrane gangliosides. PLoS ONE. 10(12)(Biology). View Reference
  3. Kirchberger S, Majdic O, Steinberger P, et al. Human rhinoviruses inhibit the accessory function of dendritic cells by inducing sialoadhesin and B7-H1 expression. J Immunol. 2005; 175(2):1145-1152. (Immunogen: Blocking, Cell separation, Flow cytometry, Functional assay, Inhibition, Western blot). View Reference
  4. Rose T, Grützkau A, Hirseland H, et al. IFNα and its response proteins, IP-10 and SIGLEC-1, are biomarkers of disease activity in systemic lupus rythematosus. Arthritis Rheum. 2013; 72(10):1639-1645. (Biology). View Reference
  5. Xiong YS, Cheng Y, Lin QS, et al. Increased expression of Siglec-1 on peripheral blood monocytes and its role in mononuclear cell reactivity to autoantigen in rheumatoid arthritis. Rheumatology (Oxford). (Biology). View Reference
  6. van den Berg TK, Nath D, Ziltener HJ, et al. Cutting edge: CD43 functions as a T cell counterreceptor for the macrophage adhesion eceptor sialoadhesin Siglec-1) . J Immunol. 2001; 166(6):3637-3640. (Biology). View Reference
すべて表示する (6) 表示項目を減らす
565248 Rev. 2

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.