Multicolor flow cytometric analysis of CD203c expression on human peripheral blood cells. Human whole blood was stained with Mouse PerCP-Cy™5.5 Anti-Human CD123 antibody (Cat. No. 560904/558714) and either APC Mouse IgG1 κ Isotype Control (Cat. No. 554681; Left Panel) or APC Mouse Anti-Human CD203c (Cat. No. 562973; Right Panel). Whole blood samples were then treated with BD FACS™ Lysing Solution (Cat. No. 349202) to lyse erythrocytes and fix the remaining leucocytes. The two-color flow cytometric dot plots show the correlated expression patterns of CD123 versus CD203c (or Ig Isotype control staining) for gated events with the forward and side-light scattering characteristics of intact lymphoid cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System. A target acquisition of at least 500 events with low side-light scatter and bright CD123 expression were included.
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Multicolor flow cytometric analysis of CD203c expression on human peripheral blood cells. Human whole blood was stained with Mouse PerCP-Cy™5.5 Anti-Human CD123 antibody (Cat. No. 560904/558714) and either APC Mouse IgG1 κ Isotype Control (Cat. No. 554681; Left Panel) or APC Mouse Anti-Human CD203c (Cat. No. 562973; Right Panel). Whole blood samples were then treated with BD FACS™ Lysing Solution (Cat. No. 349202) to lyse erythrocytes and fix the remaining leucocytes. The two-color flow cytometric dot plots show the correlated expression patterns of CD123 versus CD203c (or Ig Isotype control staining) for gated events with the forward and side-light scattering characteristics of intact lymphoid cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System. A target acquisition of at least 500 events with low side-light scatter and bright CD123 expression were included.
製品詳細
BD Pharmingen™
ENPP3; E-NPP 3; NPP3; PDNP3; PD-Ibeta; PD-1β; B10; NPPase; gp130RB13-6
Human (QC Testing)
Mouse BALB/c IgG1, κ
Human CD203c Transfected Cell Line
Flow cytometry (Routinely Tested)
5 µl
AB_2737925
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation and Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
関連製品
Stain Buffer (FBS) RUO
サイズ
500 mL
カタログ番号
554656
Lysing Solution 10X Concentrate RUO (GMP)
サイズ
100 mL
カタログ番号
349202
APC Mouse IgG1 κ Isotype Control RUO
サイズ
0.1 mg
カタログ番号
554681
PerCP-Cy™5.5 Mouse anti-Human CD123 RUO
サイズ
25 Tests
カタログ番号
560904
PerCP-Cy™5.5 Mouse anti-Human CD123 RUO
サイズ
100 Tests
カタログ番号
558714
562973 Rev. 1
抗体の詳細
NP4D6
The NP4D6 monoclonal antibody specifically binds to CD203c. CD203c is a type II transmembrane glycoprotein that is a member of the E-NNP family of ectoenzymes. CD203c is also known as ectonucleotide pyrophosphatase/phosphodiesterase 3 (E-NPP3, ENPP3) due to its capacity to hydrolyze phosphodiester and phosphosulfate bonds in a variety of molecules including deoxynucleotides, nucleoside phosphates, and nicotinamide adenine dinucleotide. CD203c is otherwise known as Phosphodiesterase-1β (PD-1β, PD-I beta), B10, or gp130RB13-6. CD203c is expressed by basophils and mast cells. Basophils increase CD203c expression following activation with allergens or antibodies that crosslink cytophilic IgE. Thus, CD203c serves as a useful flow cytometric marker for basophil activation and the detection and analysis of type 1 allergic responses. IgE-receptor cross-linking results in CD203c upregulation and overexpression on neoplastic mast cells in cases of systemic mastocytosis.
562973 Rev. 1
フォーマットの詳細
APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
APC
Red 627-640 nm
651 nm
660 nm
562973 Rev.1
引用＆参考文献
Development References (8)
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Binder M, Fierlbeck G, King T, Valent P, Buhring HJ. Individual hymenoptera venom compounds induce upregulation of the basophil activation marker ectonucleotide pyrophosphatase/phosphodiesterase 3 (CD203c) in sensitized patients. Int Arch Allergy Immunol. 2002; 129(2):160-168. (Clone-specific: Flow cytometry). View Reference
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Buhring HJ, Streble A, Valent P. The basophil-specific ectoenzyme E-NPP3 (CD203c) as a marker for cell activation and allergy diagnosis. Int Arch Allergy Immunol. 2004; 133(4):317-329. (Clone-specific: Flow cytometry). View Reference
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Fureder W, Schernthaner GH, Ghannadan M, et al. Quantitative, phenotypic, and functional evaluation of basophils in myelodysplastic syndromes. Eur J Clin Invest. 2001; 31(10):894-901. (Biology). View Reference
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Ghannadan M, Hauswirth AW, Schernthaner GH, et al. Detection of novel CD antigens on the surface of human mast cells and basophils. Int Arch Allergy Immunol. 2002; 127(4):299-307. (Clone-specific: Immunofluorescence). View Reference
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Hauswirth AW, Natter S, Ghannadan M, et al. Recombinant allergens promote expression of CD203c on basophils in sensitized individuals. J Allergy Clin Immunol. 2002; 110(1):102-109. (Clone-specific: Flow cytometry). View Reference
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Hauswirth AW, Sonneck K, Florian S, et al. Interleukin-3 promotes the expression of E-NPP3/CD203C on human blood basophils in healthy subjects and in patients with birch pollen allergy. Int J Immunopathol Pharmacol. 2007; 20(2):267-278. (Biology). View Reference
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Hennersdorf F, Florian S, Jakob A, et al.. Identification of CD13, CD107a, and CD164 as novel basophil-activation markers and dissection of two response patterns in time kinetics of IgE-dependent upregulation. Cell Res. 2005; 15(5):325-335. (Biology). View Reference
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Iwamoto T, Yuta A, Tabata T, et al. Evaluation of basophil CD203c as a predictor of carboplatin-related hypersensitivity reaction in patients with gynecologic cancer. Biol Pharm Bull. 2012; 35(9):1487-1495. (Biology). View Reference
562973 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
