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      PE Mouse anti-Human BMI-1
      PE Mouse anti-Human BMI-1
      Flow cytometric analysis of BMI-1 expression in human osteosarcoma cell line. U-2 OS cells  (ATCC; HTB-96™) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050). The cells were stained with either PE Mouse IgG1, κ Isotype Control (dashed line histogram, Cat. No. 554680) or PE Mouse anti-Human BMI-1 antibody (solid line histogram, Cat. No. 562528) at matched concentrations. The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed on a BD FACSCanto™ II Flow Cytometry System.
      PE Mouse anti-Human BMI-1
      Flow cytometric analysis of BMI-1 expression in human osteosarcoma cell line. U-2 OS cells  (ATCC; HTB-96™) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050). The cells were stained with either PE Mouse IgG1, κ Isotype Control (dashed line histogram, Cat. No. 554680) or PE Mouse anti-Human BMI-1 antibody (solid line histogram, Cat. No. 562528) at matched concentrations. The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed on a BD FACSCanto™ II Flow Cytometry System.
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      BD Pharmingen™
      BMI1; PCGF4; polycomb group ring finger 4; RING finger protein 51; RNF51
      Human (QC Testing)
      Mouse BALB/c IgG1, κ
      Human BMI-1 Recombinant Protein
      Intracellular staining (flow cytometry) (Routinely Tested)
      5 µl
      648
      AB_11153300
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation and Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
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      Product Notices

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      6. All other brands are trademarks of their respective owners.
      7. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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      562528 Rev. 1
      抗体の詳細
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      P51-311

      The P51-311 monoclonal antibody binds to human BMI-1 (B lymphoma Mo-MLV insertion region 1 homolog). BMI1 is a c-myc cooperating oncogene that encodes an ~45 kDa protein that is a member of the Polycomb Group (PcG) of proteins. PcG proteins are essential for the maintenance, but not initiation, of the transcriptionally repressed state of certain developmental genes. PcG proteins are a structurally diverse group of proteins with conserved functions from fly to human cells. PcG proteins form complexes and regulate the expression of genes involved in cell cycle, DNA repair and differentiation that are crucial for maintaining the self renewal of normal and cancer stem cells. Specifically, BMI-1 is a core component of PRC1 (polycomb repressive complex 1). BMI-1, via the up-regulation of hTERT and independent of c-myc, can immortalize mammary epithelial cells. BMI-1 has also been shown to repress the INK4A locus that controls the tumor suppressors p16 and p19ARF (mouse homologue of p14ARF) in mouse models. BMI-1 plays a role in maintaining the self-renewal capacities of stem cells including hematopoietic, intestinal, retinal and neural stem cells.  During antibody development, the purified P51-311 monoclonal antibody was found to detect BMI-1 by Western blot analysis of cellular lysates and by indirect immunofluorescent staining and flow cytometric analysis of fixed and permeabilized cells.

      562528 Rev. 1
      フォーマットの詳細
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      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      562528 Rev.1
      引用＆参考文献
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      Development References (6)

      1. Dimri GP, Martinez JL, Jacobs JJ. The Bmi-1 oncogene induces telomerase activity and immortalizes human mammary epithelial cells. Cancer Res. 2002; 62(16):4736-4745. (Biology). View Reference
      2. Itahana K, Zou Y, Itahana Y. Control of the replicative life span of human fibroblasts by p16 and the polycomb protein Bmi-1. Mol Cell Biol. 2003; 23(1):389-401. (Biology). View Reference
      3. Molofsky AV, Pardal R, Iwashita T, Park IK, Clarke MF, Morrison SJ. Bmi-1 dependence distinguishes neural stem cell self-renewal from progenitor proliferation. Nature. 2003; 6961(962):967. (Biology). View Reference
      4. Park IK, Qian D, Kiel M, et al. Bmi-1 is required for maintenance of adult self-renewing haematopoietic stem cells. Nature. 2003; 423(6937):302-305. (Biology). View Reference
      5. Sangiorgi, E., Capecchi, M. R. Bmi1 is expressed in vivo in intestinal stem cells. Nat Genet. 2008; 40(7):915-920. (Biology). View Reference
      6. Tian H, Biehs B, Warming S, et al. A reserve stem cell population in small intestine renders Lgr5-positive cells dispensable. Nature. 2011; 478(7368):255-259. (Biology). View Reference
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      562528 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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