Skip to main content Skip to navigation
APC Mouse anti-Human CD43
APC Mouse anti-Human CD43
Flow cytometric analysis of APC anti-human CD43 on human lymphocytes. Whole blood was stained with APC anti-human CD43 (clone 1G10 , Cat. No. 560198) and compared to whole blood stained with a APC mouse IgG1 isotype control (clone MOPC-21, Cat. No. 555751).   The isotype control is represented by a dashed line and the APC anti-human CD43 by the solid line. Flow cytometry was performed on a BD FACSCalibur™ System and the histograms were derived from the gated events based on light scattering characteristics of viable cells.
Flow cytometric analysis of APC anti-human CD43 on human lymphocytes. Whole blood was stained with APC anti-human CD43 (clone 1G10 , Cat. No. 560198) and compared to whole blood stained with a APC mouse IgG1 isotype control (clone MOPC-21, Cat. No. 555751).   The isotype control is represented by a dashed line and the APC anti-human CD43 by the solid line. Flow cytometry was performed on a BD FACSCalibur™ System and the histograms were derived from the gated events based on light scattering characteristics of viable cells.
製品詳細
Down Arrow Up Arrow


BD Pharmingen™
Human (QC Testing)
Mouse IgG1, κ
Flow cytometry (Routinely Tested)
20 µl
VI N-L166
6693
AB_1645460
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
560198 Rev. 1
抗体の詳細
Down Arrow Up Arrow
1G10

The 1G10 monoclonal antibody specifically binds to CD43, a 95-135 kDa sialoglycoprotein that is highly expressed on most human leucocytes. CD43 is encoded by the SPN gene and is also known as Sialophorin, Galactoglycoprotein (GALGP), Leukosialin, and Leukocyte sialoglycoprotein. The CD43 antigen is expressed on T cells, pre-B cells and activated B cells, NK cells and granulocytes, but is not present on resting peripheral blood B cells, red blood cells, platelets and non-hematopoietic cells. CD43 is enzymatically shed from leucocyte  surfaces following activation by various stimuli. CD43 appears to be involved in intercellular interactions that regulate T, B, and NK cell functions. This antibody is suitable for staining formalin-fixed, paraffin-embedded tissue sections with TUF pretreatment.

560198 Rev. 1
フォーマットの詳細
Down Arrow Up Arrow
APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
APC
Red 627-640 nm
651 nm
660 nm
560198 Rev.1
引用&参考文献
Down Arrow Up Arrow

Development References (4)

  1. Borche L, Lozano F, Vilella R, Vives J. CD43 monoclonal antibodies recognize the large sialoglycoprotein of human leukocytes. Eur J Immunol. 1987; 17(10):1523-1526. (Biology). View Reference
  2. Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
  3. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
  4. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
すべて表示する (4) 表示項目を減らす
560198 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.