Biotin Rat Anti-Mouse Ig, κ Light Chain

BD Pharmingen™ Biotin Rat Anti-Mouse Ig, κ Light Chain

Name: Biotin Rat Anti-Mouse Ig, κ Light Chain
Brand: Biotin Rat Anti-Mouse Ig, κ Light Chain
SKU: 559750

クローン 187.1 (RUO)

カタログ番号:557653

希望小売価格:¥51,810

単位:EA (1 Each)

容量:

0.5 mg

数量:

見積に追加

見積リストに保存バルク試薬見積の依頼

製品詳細

Brand: BD Pharmingen™

Reactivity: Mouse (QC Testing)

Isotype: Rat SD, also known as Sprague-Dawley (outbred) IgG1, κ

Immunogen: Mouse IgG2b κ secreted by MPC-11 plasmacytoma

Application: ELISA (Routinely Tested), Flow cytometry (Tested During Development)

Concentration: 0.5 mg/ml

RRID: AB_397314

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C.

推奨アッセイ手順

ELISA: This antibody has been tested by ELISA (2 µg/ml). For the sandwich mouse IgG1, G2a, G2b, G3, IgM, IgA, and IgE ELISA, biotin-conjugated mAb 187.1 (cocktailed with biotin-conjugated anti-mouse Ig λ1, λ2, and λ3 light chain, R26-46 mAb, Cat. No. 553433) is optimal for detection with anti-mouse Ig isotype specific mAbs (A85-3, Cat. No. 553445; R19-15, Cat. No. 553387; R12-3, Cat. No. 553392; R2-38, Cat. No. 553404; II/41, Cat. No. 553435; C10-1, Cat. No. 556960; C10-3, Cat. No. 556969; R35-72, Cat. No. 553413) for capture, respectively.

Immunofluorescent staining and flow cytometry: Biotinylated 187.1 mAb may be used as a primary or secondary reagent in immunofluorescent staining. For flow cytometric detection of intracytoplasmic Ig, κ light chain we recommend FITC-conjugated mAb 187.1 (Cat. No. 550003).

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

データシート

Technical Data Sheet (TDS)

最近閲覧済み

BD Pharmingen™ PE Rat Anti-Mouse CD3 Molecular Complex(17A2) RUO

カタログ番号 557653

559750 Rev. 6

抗体の詳細

187.1

The 187.1 monoclonal antibody specifically binds to kappa light chains of mouse immunoglobulins. The 187.1 antibody does not react with mouse λ1 or λ2 immunoglobulin lights chains or mouse immunoglobulin heavy chains.

559750 Rev. 6

フォーマットの詳細

Biotin

Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.

フォーマット: Biotin

559750 Rev.6

引用＆参考文献

Development References (1)

Yelton DE, Desaymard C, Scharff MD. Use of monoclonal anti-mouse immunoglobulin to detect mouse antibodies. Hybridoma. 1981; 1(1):5-11. (Immunogen). View Reference

559750 Rev. 6

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.