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      Purified Rat Anti-Mouse CD18
      558565
      ¥48,400
      EA (1 Each)
      0.1 mg
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      見積リストに保存バルク試薬見積の依頼
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      BD Pharmingen™
      Cd18; ITGB2; Integrin β2 chain
      Mouse (QC Testing)
      Rat SD, also known as Sprague-Dawley (outbred) IgG1, κ
      LFA-1 (CD11a/CD18) from mouse T-cell hybridoma TAM8C41
      Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Blocking, Immunoprecipitation (Reported)
      0.5 mg/ml
      AB_396703
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
      5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      データシート

      DownloadTechnical Data Sheet (TDS)
      557440 Rev. 8
      抗体の詳細
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      GAME-46

      The GAME-46 monoclonal antibody specifically binds to CD18. CD18 is the common β2 integrin chain of LFA-1 (CD11a/CD18, αLβ2 integrin), Mac-1 (CD11b/CD18, αMβ2 integrin), and gp150, 95 (CD11c/CD18, αXβ2 integrin).  Expression of CD18 is limited to leucocytes, where it is widely distributed in consort with each of the three integrin α chains (CD11a, CD11b, and CD11c).  Among splenocytes, NK cells have the highest density of CD18, and T lymphocytes express a higher density than the remaining cells.  The β2 integrins are important mediators of leukocyte-endothelium interactions.  It has been reported that GAME-46 antibody blocks in vitro adhesion of LFA-1-expressing cells to the ligands ICAM-1, ICAM-2 and ICAM-3 and of Mac-1-expressing cells to ICAM-1, C3bi, and fibrinogen.

      557440 Rev. 8
      フォーマットの詳細
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      557440 Rev.8
      引用＆参考文献
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      Development References (3)

      1. Driessens MH, van Hulten P, Zuurbier A, La Riviere G, Roos E. Inhibition and stimulation of LFA-1 and Mac-1 functions by antibodies against murine CD18. Evidence that the LFA-1 binding sites for ICAM-1, -2, and -3 are distinct. J Leukoc Biol. 1996; 60(6):758-765. (Immunogen: Blocking, Immunoprecipitation). View Reference
      2. Springer TA. Adhesion receptors of the immune system. Nature. 1990; 346(6283):425-434. (Biology). View Reference
      3. Springer TA. Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm. Cell. 1994; 76(2):301-314. (Biology). View Reference
      557440 Rev. 8

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.