Skip to main content Skip to navigation
Hamburger Menu
Search icon
Mini Cart
Empty Cartサインインされていません
Country Selector Country Selector
Japan (Japanese)
Profile Icon Profile Icon
Sign-in/Register UserName
Mini Cart
Empty Cartサインインされていません
Products

Link Arrow
Solutions

Link Arrow
Discover & Learn

Link Arrow
Resources & Tools

Link Arrow
Support

Link Arrow
Search icon Search icon
Close Menu
    Mini Cart
    Empty Cartサインインされていません
    Expand menu Expand menu
      Loading...
      Alert Icon
      PE Mouse Anti-Human CD125
      PE Mouse Anti-Human CD125
      PE Mouse Anti-Human CD125

      Flow cytometric analysis of CD125 expression on human peripheral blood eosinophils. Human eosinophils were stained with either PE Mouse Anti-Human CD125 (Cat. No. 555902; solid line histogram) or PE Mouse IgG1, κ Isotype Control (Cat. No. 555749; dashed line histogram). Fluorescent histograms were derived from gated events with the forward and side light-scattering characteristics of viable cells. Flow cytometry was carried out on  BD FACScan™ system.

      747915
      ¥78,650
      EA (1 Each)
      0.2 mg
      Add Product To Quote見積に追加
      見積リストに保存バルク試薬見積の依頼
      製品詳細
      Down Arrow


      BD Pharmingen™
      CD125; IL-5Rα; IL5RA; IL5R
      Human (QC Testing)
      Mouse IgG1, κ
      IL-5Rα-transfected cells
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      AB_396213
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation and Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
      詳細を表示 blue down arrow 表示項目を減らす blue up arrow

      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      詳細を表示 blue down arrow 表示項目を減らす blue up arrow

      データシート

      DownloadTechnical Data Sheet (TDS)
      555902 Rev. 8
      抗体の詳細
      Down Arrow
      A14

      The A14 monoclonal antibody specifically recognizes the α chain of the Interleukin-5 Receptor (IL-5Rα), which is also known as CD125. IL-5Rα is a 55-60 kDa type I transmembrane glycoprotein that is expressed on B1 B cells, eosinophils, basophils and mast cells. The IL-5Rα chain associates with CD131 (Common β-chain) which serves as the signaling common β-chain subunit of the IL-5R complex as well as for the Interleukin-3 (IL-3R) and GM-CSF receptor (GM-CSFR) complexes. IL-5 promotes the growth and differentiation of eosinophil precursors and activates mature eosinophils. Clone A14 was developed using IL-5Rα-transfected cells as immunogen. This antibody binding can be blocked by IL-5.

      555902 Rev. 8
      フォーマットの詳細
      Down Arrow
      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 561 nm
      496 nm, 566 nm
      576 nm
      555902 Rev.8
      引用＆参考文献
      Down Arrow

      Development References (2)

      1. Blom M, Tool AT, Kok PT, Koenderman L, Roos D, Verhoeven AJ. Granulocyte-macrophage colony-stimulating factor, interleukin-3 (IL-3), and IL-5 greatly enhance the interaction of human eosinophils with opsonized particles by changing the affinity of complement receptor type 3. Blood. 1994; 83(10):2978-2984. (Biology). View Reference
      2. Ingley E, Young IG. Characterization of a receptor for interleukin-5 on human eosinophils and the myeloid leukemia line HL-60. Blood. 1991; 78(2):339-344. (Biology). View Reference
      555902 Rev. 8

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.