Purified Mouse Anti-Human CD56 (NCAM-1)

BD Pharmingen™ Purified Mouse Anti-Human CD56 (NCAM-1)

Name: Purified Mouse Anti-Human CD56 (NCAM-1)
Brand: Purified Mouse Anti-Human CD56 (NCAM-1)
SKU: 555514

クローン B159 (RUO)

Flow cytometric analysis of CD56 (NCAM-1) expression on human peripheral blood lymphocytes. Whole blood was stained with either Purified Mouse Anti-Human CD56 (Cat. No. 555514; solid line histogram) or Purified Mouse IgG1 κ Isotype Control (Cat. No. 555746; dashed line histogram), followed by FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988). Erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No. 349202). Fluorescence histograms depicting CD56 (NCAM-1) (or Ig isotype expression) were derived from gated events with the side and forward light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACScan™ system.

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カタログ番号:555514

希望小売価格:¥41,360

単位:EA (1 Each)

容量:

0.1 mg

数量:

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製品詳細

Brand: BD Pharmingen™

Alternative Name: NCAM1; NCAM-1; Neural cell adhesion molecule 1; NCAM; NKH1; MSK39

Reactivity: Human (QC Testing)

Isotype: Mouse IgG1, κ

Immunogen: Human NK Cells

Application: Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development)

Concentration: 0.5 mg/ml

Workshop Number: V NK75

RRID: AB_395904

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

データシート

Technical Data Sheet (TDS)

関連製品

Purified Mouse IgG1, κ Isotype Control RUO

サイズ 0.1 mg カタログ番号 555746

FITC Goat Anti-Mouse IgG/IgM RUO

サイズ 0.5 mg カタログ番号 555988

555514 Rev. 10

抗体の詳細

B159

The B159 monoclonal antibody specifically binds to CD56. CD56 is a heavily glycosylated adhesion protein that is present on a subpopulation of peripheral blood large granular lymphocytes that demonstrate natural killer activity. CD56 is also expressed on a subset of T cells but is not expressed on myeloid cells, erythrocytes or B cells. This antigen is a pan-NK-cell marker. CD56 is virtually identical to an isoform of the neural cell adhesion molecule (NCAM), a structure mediating homotypic and heterotypic cell-cell interactions.

555514 Rev. 10

フォーマットの詳細

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

フォーマット: Purified

555514 Rev.10

引用＆参考文献

Development References (7)

Bennett IM, Zatsepina O, Zamai L, Azzoni L, Mikheeva T, Perussia B. Definition of a natural killer NKR-P1A+/CD56-/CD16- functionally immature human NK cell subset that differentiates in vitro in the presence of interleukin 12. J Exp Med. 1996; 184(5):1845-1856. (Biology: Flow cytometry). View Reference
Crotta S, Stilla A, Wack A, et al. Inhibition of natural killer cells through engagement of CD81 by the major hepatitis C virus envelope protein. J Exp Med. 2002; 195(1):35-41. (Clone-specific: Flow cytometry). View Reference
Maraiani E, Cattini L, Piacentini A, Sgobbi S, Facchini A. Distribution of Workshop NK-cell and CD56 mAb in human peripheral blood lymphocytes during aging. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:1394-1397.
Ritz J, Trinchieri G, Lanier LL. NK-cell Antigens: Section Report. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:1367-1372.
Robertson MJ, Cochran KJ, Ritz J. Characterization of surface antigens expressed by normal and neoplastic human NK cells. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:1374-1377.
Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.

すべて表示する (7)

555514 Rev. 10

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.