Skip to main content Skip to navigation
Biotin Mouse Anti-Human CD122
Biotin Mouse Anti-Human CD122
Flow cytometric analysis of CD122 expression on human peripheral blood lymphocytes. Whole blood was stained with either Biotin Mouse Anti-Human CD122 (Cat. No. 554524; solid line histogram) or Biotin Mouse IgG1 κ Isotype Control (Cat. No. 550615; dashed line histogram), then PE Streptavidin (Cat. No. 554061). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Fluorescent histograms were derived from gated events with the forward and light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACScan™ system.
Flow cytometric analysis of CD122 expression on human peripheral blood lymphocytes. Whole blood was stained with either Biotin Mouse Anti-Human CD122 (Cat. No. 554524; solid line histogram) or Biotin Mouse IgG1 κ Isotype Control (Cat. No. 550615; dashed line histogram), then PE Streptavidin (Cat. No. 554061). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Fluorescent histograms were derived from gated events with the forward and light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACScan™ system.
製品詳細
Down Arrow Up Arrow


BD Pharmingen™
IL2RB; IL-2Rβ; IL-2R subunit beta; IL-2RB; IL15RB; IL-2/15Rb; IL-2R p75
Human (QC Testing)
Mouse BALB/c IgG1, κ
Human YTS Cell Line
Flow cytometry (Routinely Tested)
0.5 mg/ml
V C046
3560
AB_395452
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

推奨アッセイ手順

This antibody has also been reported to precipitate from YTS cell lysates a 90,000 kDa complex (IL-2 + p75). This antibody has been reported not to block IL-2 binding to the β chain of the IL-2 receptor. Please note that these applications are not routinely tested at BD Biosciences.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
554524 Rev. 2
抗体の詳細
Down Arrow Up Arrow
Mik-β3

The Mik-β3 monoclonal antibody specifically binds to CD122. CD122 is also known as IL-2/15Rβ because it is the shared p75 subunit (IL-12Rβ/IL-15Rβ) of heteromeric receptors for IL-2 and IL-15. CD122 is a 70-75 kD type I transmembrane glycoprotein expressed on  thymocytes, T cells, B cells, NK cells, monocytes, hematopoietic progenitor cells, fetal liver cells and stromal cells. The CD122 plays roles in the activation, differentiation and proliferation of various cell types including T cells, B cells and NK cells.

554524 Rev. 2
フォーマットの詳細
Down Arrow Up Arrow
Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
554524 Rev.2
引用&参考文献
Down Arrow Up Arrow

Development References (4)

  1. Guesdon JL, Ternynck T, Avrameas S. The use of avidin-biotin interaction in immunoenzymatic techniques. J Histochem Cytochem. 1979; 27(8):1131-1139. (Biology). View Reference
  2. Ishikawa T, Uchiyama T, Kamio M, Onishi R, Kodaka T, Okuma M. IL-4 down-regulates IL-2 receptor p75 by accelerating its endocytosis. Int Immunol. 1991; 3(6):517-525. (Biology). View Reference
  3. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
  4. Tsudo M, Kitamura F, Miyasaka M. Characterization of the interleukin 2 receptor beta chain using three distinct monoclonal antibodies. Proc Natl Acad Sci U S A. 1989; 86(6):1982-1986. (Biology). View Reference
すべて表示する (4) 表示項目を減らす
554524 Rev. 2

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.