Biotin Mouse Anti-Rat IgG1

BD Pharmingen™ Biotin Mouse Anti-Rat IgG1

Name: Biotin Mouse Anti-Rat IgG1
Brand: BD Pharmingen™
SKU: 553890

クローン RG11/39.4

(RUO)

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製品詳細

Brand: BD Pharmingen™

Reactivity: Rat (QC Testing)

Isotype: Mouse SJL IgG2a, κ

Immunogen: Pooled rat IgG1

Application: ELISA (Routinely Tested), Flow cytometry (Tested During Development)

Concentration: 0.5 mg/ml

RRID: AB_395119

Storage Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

推奨アッセイ手順

For the sandwich rat IgG1 ELISA, biotinylated mAb RG11/39.4 is optimal for detection with purified anti-rat IgG1 mAb B46-2 (available by special order) for capture. Biotin-conjugated RG11/39.4 mAb may be used as a secondary reagent in immunofluorescent staining RG11/39.4 antibody is effective for detection of cell-surface or intracellular Ig by immunofluorescent staining with flow cytometric analysis. For flow cytometric detection of intracytoplasmic IgG1, we recommend FITC-conjugated RG11/39.4 mAb (Cat. No. 553892). For immunohistochemical staining, we recommend the use of biotinylated anti-rat IgG1/2a mAb G28-5 in our special formulation for immunohistochemistry, Cat. No. 550325.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

関連製品

FITC Mouse Anti-Rat IgG1 RUO

サイズ 0.5 mg カタログ番号 553892

553890 Rev. 2

抗体の詳細

RG11/39.4

The RG11/39.4 antibody reacts specifically with the Fc region of rat IgG1. It does not react with other Ig isotypes.

553890 Rev. 2

フォーマットの詳細

Biotin

Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.

フォーマット: Biotin

553890 Rev.2

引用＆参考文献

Development References (1)

Springer TA, Bhattacharya A, Cardoza JT, Sanchez-Madrid F. Monoclonal antibodies specific for rat IgG1, IgG2a, and IgG2b subclasses, and kappa chain monotypic and allotypic determinants: reagents for use with rat monoclonal antibodies. Hybridoma. 1982; 1(3):257-273. (Immunogen: ELISA). View Reference

553890 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.