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Biotin Rat Anti-Mouse CD138
Biotin Rat Anti-Mouse CD138
Flow cytometric analysis of CD138 expression on mouse bone marrow B lymphocytes. C57BL/6 bone marrow leukocytes were stained with Biotin Rat Anti-Mouse CD138 (Cat. No. 553713, Right Panel) and FITC Rat Anti-Mouse CD45R/B220 (Cat. No. 553087/553088, Both Panels), followed by PE Streptavidin (Cat. No. 554061, Both Panels). Two-color contour plots are derived from gated events with the side and forward light-scattering characteristics of viable leukocytes. Flow cytometry was performed on a BD FACScan™ system.
Flow cytometric analysis of CD138 expression on mouse bone marrow B lymphocytes. C57BL/6 bone marrow leukocytes were stained with Biotin Rat Anti-Mouse CD138 (Cat. No. 553713, Right Panel) and FITC Rat Anti-Mouse CD45R/B220 (Cat. No. 553087/553088, Both Panels), followed by PE Streptavidin (Cat. No. 554061, Both Panels). Two-color contour plots are derived from gated events with the side and forward light-scattering characteristics of viable leukocytes. Flow cytometry was performed on a BD FACScan™ system.
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BD Pharmingen™
SYND1; Syndecan-1; syn-1; Sdc1; Sstn; synstatin
Mouse (QC Testing)
Rat F344, also known as Fischer, CDF IgG2a, κ
NAMRU mouse mammary gland epithelial cell line NMuMG
Flow cytometry (Routinely Tested), Immunofluorescence, Immunohistochemistry-frozen (Reported)
0.5 mg/ml
20969
AB_394999
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation and Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

推奨アッセイ手順

CD138 is expressed at low density on cells in the normal bone marrow; therefore, we recommend the use of "bright" second-step reagent, such as Streptavidin-PE (Cat. No. 554061). Other reported applications include immunofluorescence microscopy and immunohistochemical staining of frozen sections.  

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. An isotype control should be used at the same concentration as the antibody of interest.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
553713 Rev. 12
抗体の詳細
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281-2

The 281-2 monoclonal antibody specifically binds to the core protein of CD138 (Syndecan-1), a cell-surface, integral membrane heparan sulfate- and chondroitin sulfate-containing proteoglycan that binds to interstitial extracellular matrix molecules.  Syndecan-1 is predominantly expressed on epithelial cells, where its expression correlates with normal epithelial organization.  It is also expressed on B lymphocytes at specific stages during their differentiation: precursor B cells in the bone marrow, and antibody-secreting cells including plasma cells (but not mature peripheral B cells).  It is thus implicated in mediating B cell-matrix interactions.  CD138 expression is also regulated during embryonic development, and the molecule shows a tissue- specific structural polymorphism resulting from different post-translational  modifications.  The 281-2 antibody may be used to detect the differently glycosylated forms, because it reacts with the core protein.  Furthermore, the mAb detects the Syndecan-1 ectodomain which is cleaved from cell surfaces by a metalloproteinase.

553713 Rev. 12
フォーマットの詳細
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Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
553713 Rev.12
引用&参考文献
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Development References (8)

  1. Driver DJ, McHeyzer-Williams LJ, Cool M, Stetson DB, McHeyzer-Williams MG. Development and maintenance of a B220- memory B cell compartment. J Immunol. 2001; 167(3):1393-1405. (Biology: Immunofluorescence). View Reference
  2. Fitzgerald ML, Wang Z, Park PW, Murphy G, Bernfield M. Shedding of syndecan-1 and -4 ectodomains is regulated by multiple signaling pathways and mediated by a TIMP-3-sensitive metalloproteinase. J Cell Biol. 2000; 148(4):811-824. (Biology: Immunofluorescence). View Reference
  3. Jalkanen M, Nguyen H, Rapraeger A, Kurn N, Bernfield M. Heparan sulfate proteoglycans from mouse mammary epithelial cells: localization on the cell surface with a monoclonal antibody. J Cell Biol. 1985; 101(3):976-984. (Immunogen). View Reference
  4. Lalor PA, Nossal GJ, Sanderson RD, McHeyzer-Williams MG. Functional and molecular characterization of single, (4-hydroxy-3-nitrophenyl)acetyl (NP)-specific, IgG1+ B cells from antibody-secreting and memory B cell pathways in the C57BL/6 immune response to NP. Eur J Immunol. 1992; 22(11):3001-3011. (Biology). View Reference
  5. Sanderson RD, Lalor P, Bernfield M. B lymphocytes express and lose syndecan at specific stages of differentiation. Cell Regul. 1989; 1(1):27-35. (Biology). View Reference
  6. Sanderson RD, Sneed TB, Young LA, Sullivan GL, Lander AD. Adhesion of B lymphoid (MPC-11) cells to type I collagen is mediated by integral membrane proteoglycan, syndecan. J Immunol. 1992; 148(12):3902-3911. (Biology). View Reference
  7. Saunders S, Jalkanen M, O'Farrell S, Bernfield M. Molecular cloning of syndecan, an integral membrane proteoglycan. J Cell Biol. 1989; 108(4):1547-1556. (Biology). View Reference
  8. Wehrli N, Legler DF, Finke D. Changing responsiveness to chemokines allows medullary plasmablasts to leave lymph nodes. Eur J Immunol. 2001; 31(2):609-616. (Biology: Immunohistochemistry). View Reference
すべて表示する (8) 表示項目を減らす
553713 Rev. 12

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