Biotin Rat Anti-Mouse IgM

BD Pharmingen™ Biotin Rat Anti-Mouse IgM

Name: Biotin Rat Anti-Mouse IgM
Brand: BD Pharmingen™
SKU: 553436

クローン II/41

(RUO)

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製品詳細

Brand: BD Pharmingen™

Reactivity: Mouse (QC Testing)

Isotype: Rat IgG2a, κ

Immunogen: Not reported

Application: ELISA Detection (Routinely Tested), Flow cytometry (Tested During Development)

Concentration: 0.5 mg/ml

Entrez Gene ID: 16019

RRID: AB_394856

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

推奨アッセイ手順

Flow cytometry: For flow cytometric detection of intracytoplasmic IgM, FITC anti-mouse IgM (clone II/41) (Cat. No. 553437) is recommended.

ELISA: This antibody, coupled with avidin-HRP (Cat.No. 554058), may be used at ~2 µg/ml as the detection antibody for the detection of mouse IgM by indirect ELISA. For the detection of mouse IgM by sandwich ELISA, purified rat anti-mouse IgM (clone II/41) (Cat. No. 553435) is recommended for capture coupled with biotin-conjugated rat anti-mouse IgM (clone R6-60.2) (Cat. No. 553406) and avidin-HRP (Cat.No. 554058) for detection. Purified mouse IgM (Cat.No. 553472) may be used as the ELISA standard for either the indirect or sandwich ELISA.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

関連製品

Biotin Rat IgG2a κ Isotype Control RUO

サイズ 0.25 mg カタログ番号 553928

FITC Rat Anti-Mouse IgM RUO

サイズ 0.5 mg カタログ番号 553437

Avidin FITC RUO

サイズ 0.5 mg カタログ番号 554057

553436 Rev. 11

抗体の詳細

II/41

The II/41 clone has been reported to react specifically with mouse IgM of Igh-C[a] and Igh-C[b] haplotypes. It has been reported not to react with other Ig isotypes. In addition, the II/41 clone has been reported not to stimulate B-cell proliferation.

553436 Rev. 11

フォーマットの詳細

Biotin

Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.

フォーマット: Biotin

553436 Rev.11

引用＆参考文献

Development References (1)

Laszlo G, Hathcock KS, Dickler HB, Hodes RJ. Characterization of a novel cell-surface molecule expressed on subpopulations of activated T and B cells. J Immunol. 1993; 150(12):5252-5262. (Biology). View Reference

553436 Rev. 11

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.