PE Rat Anti-Mouse Vβ4 T-Cell Receptor

Consider BD Horizon RealYellow™ 586 (RY586) Reagents, a bright and clean fluorochrome alternative to PE off the yellow-green laser. RY586 can be used alongside PE on spectral cytometers. More Info

BD Pharmingen™ PE Rat Anti-Mouse Vβ4 T-Cell Receptor

Name: PE Rat Anti-Mouse Vβ4 T-Cell Receptor
Brand: BD Pharmingen™
SKU: 553366

クローン KT4

(RUO)

Two-color analysis of the expression of Vβ4 TCR on peripheral T lymphocytes. SJL lymph node cells were incubated simultaneously with PE-conjugated KT4, FITC-conjugated RM4-5 (anti-mouse CD4, Cat. no. 553046/553047), and FITC-conjugated 53-6.7 (anti-mouse CD8a, Cat. no. 553030/553031) monoclonal antibodies. Flow cytometry was performed on a FACScan™ (BDIS, San Jose, CA).

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製品詳細

Brand: BD Pharmingen™

Reactivity: Mouse (QC Testing)

Isotype: Rat SD, also known as Sprague-Dawley (outbred) IgG2b, κ

Immunogen: KT4 B10.D2 mouse T-cell clone I3

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

RRID: AB_394812

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation and Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

推奨アッセイ手順

For flow cytometry of cell suspensions from peripheral lymphoid tissues, it is recommended that multicolor staining be performed to distinguish T lymphocytes from non-T cells.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

関連製品

PE Rat IgG2b, κ Isotype Control RUO

サイズ 0.1 mg カタログ番号 553989

FITC Rat Anti-Mouse CD4 RUO

サイズ 0.1 mg カタログ番号 553046

FITC Rat Anti-Mouse CD8a RUO

サイズ 0.1 mg カタログ番号 553030

553366 Rev. 11

抗体の詳細

KT4

The KT4 antibody specifically recognizes the Vβ4 T-cell Receptor (TCR) of mice having the a (e.g., C57BR, C57L, SJL, SWR), b (e.g., A, AKR, BALB/c, CBA, C3H/He, C57BL, C58, DBA/1, DBA/2), and c (e.g., RIII) haplotypes of the Tcrb gene complex. Vβ4 TCR-bearing cells are among the CD4+ autoreactive T cells which induce autoimmune thyroiditis after elimination of regulatory (CD5-bright) T-cell subsets. Plate-bound KT4 antibody activates Vβ4 TCR-bearing T cells.

553366 Rev. 11

フォーマットの詳細

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

フォーマット: PE

励起源: Yellow-Green 488 nm, 532 nm, 561 nm

最大励起波長: 496 nm, 566 nm

最大蛍光波長: 576 nm

553366 Rev.11

引用＆参考文献

Development References (3)

Katz JD, Benoist C, Mathis D. T helper cell subsets in insulin-dependent diabetes. Science. 1995; 268(5214):1185-1188. (Biology). View Reference
Sugihara S, Fujiwara H, Shearer GM. Autoimmune thyroiditis induced in mice depleted of particular T cell subsets. Characterization of thyroiditis-inducing T cell lines and clones derived from thyroid lesions. J Immunol. 1993; 150(2):683-694. (Biology). View Reference
Tomonari K, Lovering E, Spencer S. Correlation between the V beta 4+ CD8+ T-cell population and the H-2d haplotype. Immunogenetics. 1990; 31(5-6):333-339. (Immunogen). View Reference

553366 Rev. 11

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.